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Hop. April 17th 1864.

I painted red line on long-shoot, which had not wound round stick & then let it wind, & then the red line became spirally wound, when the stem had wound round, & this would not happen with tape, (painted on one side), (which is best illustration,) if the end of tape had been free — The lower internodes were already twisted a little which shows that that such twisted stems can twine: Mikania scandens thus painted on convex side, also showed red line spirally twisted: so in these 2 cases result different from tendrils & there is torsion.—

[Dv]

I did the same ie red line to the Ceropegia, & the red line was first external & then internal, & then external when spin completed.— So probably in all cases torsion during act of winding —

(E

Ap. 18th I painted convex side of penultimate internode next day, as it rotated the convex side was straight & in afternoon became concave & sometime lateral; so flexure changes as shoot rotates.

Ap. 20. In the Convolvulus — Malvern the shoots were bent nearly horizontally, but this is not owing to weight as I proved. I painted black line on convex surfaces & during the revolutions of 2 shoots thus treated the black line became straight & lateral & or quite concave

The line on the pen-ultimate & ultimate inter-node did not always agree in flexure. Both certainly moved & to a certain extent independently

Hence whole shoot assumed such varied positions during revolution. This movement of whole length of shoot is the great fact.

[2bv]

Kidney Bean. April 29th 64.

I painted with red line on convex surface of 2 terminal shoots, & repeatedly I observed the red line on concave & then on convex side, & then on lateral side showing beyond doubt that whole curvature changed.

Red line painted on stem, became spiral when curled round stick.— — When arrested by stick, sometimes rose a little; exerted but little force & sprung forward, but very little, when stick removed. — Glycina arrested spring forward considerably