[27]

Feb 25 – 1862. Monochætum ensiferum.

Worsted means old flowers with pistil straight

Black means pollen of yellow anthers & nearest of their age

White means pollen of crimson anther & furthest off at their age

Silk means young flowers with pistil bent

Black means pollen of yellow anther & most distant

White means [pollen] of crimson anther nearest

Feb. 25th. I crossed 2} with Black worsted –

March 1. 1} 7 altogether (3)

= 5th 2}

6 2

28 – 2} with white worsted

28th 1} do do

March 5 2}

6 2} – 7 altogether

(over)

The principle I go on is to cross, in old & young, the pistil with pollen of remote & near anthers: in old flowers the crimson anthers are the most remote & ought to be most fertile: in young flowers the yellow anthers are the most remote& ought to be most fertile

[27v]

The early lot which were done with black & white Thread were, I believe all except one or two young flowers with rectangularly bent pistil

(8) some of them were fertilised twice; & some only once – but equal numbers so treated =

N.B. Crimson anthers very much more pollen then yellow anthers.

Black & wh silk 8

Worsted 14

Thread 16

[total] 38 [minus] 13 [=] 25

[28]

Feb 25 2 Black silk}

March 7 2 do}

4 total} young flowers

Feb 25th 2 white silk}

March 1. do}

- 7 1. white Bobbin instead of silk}

4 total} young flowers

March 7th picked off all buds & other flowers, except on bud.

March 16 I have now some several days ago noticed 5 black string dropped, including 1 worsted & I think only one black silk – not one white that I have seen has dropped.

March 17th 1 black silk – 2 black cotton = 3 Black

= 2 white thread & 1 white worsted = 3 white

March 23 1 white silk dropped & 2 bl thread dropped

5

3

1

[total] 9 bl 4 white

[28v]

Mr Turnbull's fine plant set not one pod.

April 16th I have compared as carefully as I could pollen of yellow & purple anthers & there does not seem any great difference in quantity, though the anthers so different in size – In flower just opened pollen of purple anther containing mature & was dropping on leaves hardly opened X petal; yet we see from Mr Turnbull's plant does fertilise itself –

Pollen of yellow anther apparently equally mature, possibly not quite so mature. Can the yellow anther shed first? for I formally thought that much more in crimson anther.

Ap 17 Pistil still XX completely rectangular & yellow anther shed pollen when touched so certainly mature at nearly same period.

"On the relation of the pollen of the two sets of the stamen in Monochætum ensiferum to the pistil, at its two different periods of growth & its two positions."