[247]

July 30th 11° a.m tied up to stick. 4 flower Heads of T. subterraneum to see if flower will bend down— one under Skylight did not, but then may not have been fertilised—

Also covered under Bell-glass 3 Heads of T. repens to see if not-fertilised flower-Heads will bend down.

Aug. 23d the flowers-Heads on these protected & therefore unfertilised or perhaps partially self-fertilised Heads are turned down normally.

(3

Trifolium subterraneum

July 30th 3 young flower-Heads were tied to sticks so close that they cd not bend down.—

Aug. 5' withered & examined.— (1) had 2 flowers bent down & surrounded top of peduncle— some of the central aborted flower had bent in same direction & 2 others horizontal, as the thread prevented them bending further down; if the flowers had been in natural position, they wd have bent into same position, so that it the bending certainly is not due to geotropism or apogeotropism, but to innate tendency. (Epinasty?)

(Epinasty)

— Two other tied flower Head cd not bend down their flower-heads on account of the threads & sticks, but they were extended horizontally so had moved as much as they could in proper direction i.e. towards the top of peduncle.— One flower-Head under skylight did not bend down, but the flower seem to do badly there & perhaps did not set.—

[3v]

The long tapering divisions of calyx of perfect flowers are very hairy.

There are similar short— multicellular hair on base of calyx of perfect flowers

(4

Augt 5' dug up the flower-Head which buried itself on July 22d. — The aborted flower Had become perfectly white & though bore were very few long simple Hairs, & except one young one (& this was affected by C. of Ammonia) had none of the multicellular Hairs. The outer one had become much developed into long— rigid cylinder, bearing 5 points & had grown beneath the soil, by which mean they grow had entangled the earth & formed a ball — from which the earth cd not easily be washed This must have been chiefly due to the points after growing have bending upwards & this would tend to drag flower-Head deeper in earth. — an admirable protection to the 3 included true flowers with seeds much enlarged.— Then looked again at the young aborted flowers on Heads not buried & on their summits, before the long 5-clawed projections had been developed, there were an abundance of the short, cylindrical, multicellular, hair containing much white protoplasm which aggregates under influence of C. of Ammonia 2 gr to 1 oz weakened by water under covering glass: the cells seem at first filled with

Aggregation of [illeg]

[4v]

irregular mass of protoplasm, with the lining somewhat shrunk from wall— then (in a particular cell) aggregated into 3 spheres, one pear-shaped— these united into an irregularly spherical ball, which afterwards broke up into rounded granules still partially united.

Aggregation

(5

Trifolium subterraneum

Aug. 24th. — examined some flower-Heads which had been buried for very nearly 1 month.— They do not sink deep— 1 was very nearly 1/4 of inch beneath surface, measured from base of upturned rudimentary flower, to surface of soil.— (I think seeds are larger now nearly ripe compare seeds in packet with those of some other species)— The axis of flower-head is more elongated than in the sub-arm flower Heads—

Look to Vaucher & Lecoq

The 5 arms or representative of calices, are very tough, rather rigid & elastic, & protect the seeds well, both underground & in air —(for they rot in air)— There are Hairs, roughened all over with points, on the subterranean, & sub-aerial aborted flowers— I think that these are affected by weak .sol. of C. of Ammonia — Probably absorb water anyhow.— The calices protect sees of sub-aerial flower-Heads