(A

July 8th. 1877. Desmodium gyrans

12° cleaned left side (opposite side accidental brown spot) with camel-Hair & water at 90°— After syringed surface & placed on 7 drops (from dropper) of pure water.

At same time 7 drops of same water on glass Plate closely by— This latter in watch-glass the water spread out to thrice the area & so wd catch much more dust.

July 13th 12° removed drop & let leaf free— The leaf as a whole does not seem injured, but where water lay a few brown speckles which certainly were of not there before.— The places were almost continuously covered with water.

(There was only a drop of fluid left on leaf, & this did not contain more or so much matter as when dried, as the corresponding water in watch-glass— I do not believe my former statements, or if true due to action of ether.

July 14th. 8° A.m. whole surface where water lay yellowish with brownish speck increased, & line on each side of mid- ri -rib— now quite brown. — N.B. on night 15' leaf well asleep, showing not injured.)

(July 19th 8° A.m. cut off leaf & examined— places quite decayed through & transparent. F. cut slice places — epidermic cells rotted & brown.—

Chlorophyll. pulpy & yellowish green.— Vein brownish.— separate cells killed.

N. B as only 1 side of leaf sponged I may have thus injured epidermis.)

(B

Desmodium gyrans

July 14th 1877

11° 30' A.m.

Cleaned whole upper surface of 2 youngish leaves with sponge & water at 90° & then placed 2 drops of water on one leaf & one on the second on each leaf covering only a small space; & this will show whether the sponging has injured leaves.—

July 21. 8° A.m. Traces of yellowish brown, especially on veins of all 3 leaves.— (I think some yellow yesterday, but this required a week: yet it must be admitted that if this duration kills portion of a leaf, a shorter duration wd be in some degree injurious.)

July 24th. 9° A.m. Left off putting on water: the one spot on one leaf & the 2 spots on the 2d leaf where water lay have the veins browned & surface discoloured. no dark — Not very young leaves. White wool. —)

July 25. One of the leaves well asleep, showing not injured

— 26. Brown marks very plain. —

Both asleep

Aug. 2d examined one of with white wools; the brown part keep as distinct & I can see that some of the epidermic cells are filled with brown matter & in other the walls alone.—

(zzz) see F. description of slices.

[see CUL-DAR209.12.123]