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July 8th 1881 Desmodium gyrans

2 youngish leaves on young plant (9 inches high) washed with sponge 4 drops of water transversely with water about 96° F & distilled water to be placed opposite pins on one side of leaf—; the opposite side left as contrast— leaves with one fine pin to keep horizontal above cork tablet below. —

Under Bell-glass — air damp with towel

In Hot-house— drops placed at 11. 30' July 8th (a very dark cloudy day)

July 9th morning bright & hot sunshine— afternoon cloudy, but not very dark.

(N.B after sun had shone some time splendid case of apoheliotropism, as all leaves under the Bell-glass rose vertically up (except 2 pinned ones) giving bush a very odd appearance—

Sun became dull & wind descended, & subsequently rose again.)

July 10' Days alternately bright sun & cloudy —all afternoon very bright

— 11' all morning very bright 12th & 13th blazing hot days.

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July 14'

These 2 leaves marked white thread — The upper & younger one had drops on right side (viewed from apex) The lower & older leaf on left side with drops—

July 14' 8° a.m after 5 days mostly very bright & hot,— Upper & younger leaf a little yellowish where water lay — Older leaf not at all affected.—

(15th one of these leaves which had been pinned horizontally over cork, now stood vertically from paraheliotropism, showing not in the least injured.)

22d 11° a.m. These 2 leave show no signs of having been in the least affected by the drops.— 27th do.

July 16, 17 18, 19th blazing hot & bright days

20th Dull all morning & cool— brightish in afternoon

21. Bright all day, but not very hot.—

22d Cold & Cloudy.

23d Cloudy; not very cold—

24. Sometimes cloudy sometimes bright, not cold:

25. do do do, rather warm—

26 & 27 cloudy & very cold

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July 14th 8° am Cleaned leaf with S Ether — both sides near basal & apical ends & these instantly washed with water— put on 2 drops on left side viewed from apex.— marked Cork E

A younger leaf do— on right side drops— Places marked with pins: 15th 8°. a.m. One of these leaves was speckled with minute white marks., as sometimes occurs & yet was perfectly healthy & now these minute white speckles, where water lies have all turned brown & there was trace of this yesterday evening.

[in margin:] Young Plant

July 22d 11° a.m There can be no doubt that with the older & lower of the 2 leaves that the water going over the places injured by ether has increased the injury— I can see no such effect in upper leaf

July 27th The older leaf certainly more injured on water-side, but some injury on opposite side which was washed by ether & no drops on. The only remarkable fact is that the tissues surrounding the veins especially injured.

The younger leaf also certainly more injured where water lay & especially close to veins & midrib.

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1881 Desmodium

July 15' 8 30' a.m — in Hot Case

Old Plant — 2 leaves red wool. —right-hand leaf (looking from apex) with 2 spaces on right side syringed for several minutes with water at 96°— till water adhered— Left-hand leaf one space on right side similarly syringed

20th no trace of effect— leaf left dry & not under Bell glass.—

(July 22d no effect— now added water in drops at 10° 30' am

Aug 2d. noon— I can see no effect from the drops of water on these 2 leaves.

— 6th (with 2 days intermission) there is some injury, but not more on side with drops than on other side.—

Aug. 16th. Right-hand. leaf (over) (z)

Ether by itself

15th 8°. 30' a.m Old Plant 2 leaves Black wool— right 2 spots on right side (viewed from apex) cleaned with S. Ether & instantly washed with plain water & then left dry & not under Bell-glass.

19th 20th (& 27th) on upper leafl leaf 2 traces of injury on upper leaf on right side — & on upper lower leaf, perhaps one spot injured & perhaps a trace of 2d injured space.— So Ether by itself injures leaves— very doubtful whether anything to do with mere remnant of Bloom. —

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In all these experiments during July 1881 of plants not under Bell-Glass — drops were put on leaves at about 7° 15' a.m. about 10° — 12° 15' — 2° 15' 4° 15' & sometimes 7°— 15 —

Therefore 5 or 6 times daily. This was continued for stated number of days generally for a week, but as leaves of plant growing naturally often last for 2 or 3 months, they wd often be exposed, to many more alternations of wet & drying, than those in above my experiments)

(z) with 2 spaces where water lay a very little more injured than on opposite side & this best seen when under side examined.

Left-hand leaf where water lay on one space alone, this space not injured, whereas opposite side where no water lay injured. — All this tremendously opposed to water causing any injury.

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1881 Desmodium Old Plant

July 20' 11° 30' a.m. 2 P.m. 2 youngish leaves a little spotted with white laid not pinned on cork & syringed with water at about 100° transversely & equally across middle— Black Threads

Plant placed in hot-case not under Bell-glass— one drop placed on side of pin on one side of mid-rib— These 2 drops on the 2 leaves will be alternately dried & added to.— to see if this is cause of injury— will act like warm rain.—

July 26th. Trace of Some injury on one leaf (on left side) at where water has lain

30th — This latter leaf & rt. side leaf show some burning along mid-ribs— These 2 leaves now quite freed.

Aug 2d — Left leaf with large semi circles — with green almost discharged & midrib brownish. — Right leaf with mid-rib brown, but the side on which drops rested not more injured than opposite side— Drops discontinued on morning of Aug' 3d. till 5' (2 days intermission)— Aug. 6' Both leaves somewhat injured & browned near midrib, but not worse on water than on opposite side. (over X)

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X

Aug 16th one leaf is a little more injured on one than on the other side; but neither leaves show any conspicuous effect from the water lodging on them, in comparison with the opposite & cleaned side.─