[104]

Mimosa albida

July 14 morning — 1877

Cleaned A & A whole upper surface with sponge & water at 90°— after the leaflets had dried I put on drops of distilled water, but as these remained globular [sketch]

I rubbed leaf with finger & water & then the drops adhered.

Cleaned B & B whole upper surface with pumice & water at 90° & when dry put on each drops of distilled water which seemed to adhere well.

C.C nothing done to.—

Leaflet pinned on board.

(July 18th 5° P.m. The leaflets B & B where water lies slightly browned, & some other prominent parts where the abrasion has been stronger. July 20th 8' a conspicuous brown mark on spot where water lay.)

(July 18th. AA, 8° A.m. – Certainly where water lay on leaves AA. several large spots— of yellowish colour.

(July 21' 8° A.m. gathered 1 of leaves A & F. has made section large spots almost coalescent yellowish white. F. has made sections, epidermic cells not injured, F. has looked again & feels sure that epidermis not injured. but some of hairs broken; pallisade pallisade cells more or less empty & colour of chlorophyll somewhat changed. No starch in any of the leaves.

(over)

[104v]

July 23d

2d leaf A gathered— a considerable space quite colourless, transparent, with all chlorophyll destroyed

[105]

Mimosa albida 1877

Sept 2d.

Syringed with intervals as squirt not large, for 3' nice young leaf with water at 54°: the 2 pinnæ (which stood at angle of 92° with one another) approached each other by 20°. The primary petioles fell (which stood at 117° with stem) fell 19°

The pinnæ became depressed but did not measure how much; before they formed a very large angle with primary & some almost horizontal — After interval of about 10'-15' before leaf had recovered this leaf was syringed with water at 90° for 3.', & now the pinnæ closed by 21° additional degrees.

The primary petiole fell by 10° additional degrees; & all the leaflets fell at right angles to primary primary petiole pinnæ — The basal leaflets clasped the distal pair, but these did not touch each other as in sleep.

The leaf assumed this position in 5'-8', & in from 30' to 45' had recovered normal position

[105v]

I might show position on same diagram by dotted lines after hot warm te water{

[105b]

Mimosa albida

Same time & manner as last.

A rather older but nice young leaf was syringed with water at 90°. The 2 pinnæ (which stood at 85°, approached each other by 16°

The primary petiole fell 10°: The sub-petioles or pinnæ, (which originally formed with primary petioles angle) of 149°, fell 30°, (so that they now stood with respect to primary petiole at angle of' 119; in the case in last page they stood at angle of only 90°)

In this specimen the leaflets did not approach each other nearly so much as in last case

[annotated sketch]

On 2d page of diagrams — the lines parallel to red show relative position of axis, primary petiole, & pinnæ before being syringed, & blue lines show position after being syringed.

I might give diagram of 1st leaf by assuming that pinnae formed angle of 149° with primary & after syringing an angle of 90°.

[106]

Sept 5.' 11° A.m. Mimosa albida 1877.

Sponged with water 88° 3 leaflets on 2 leaves, (ie 6) & put on with much difficulty spheres on the 6 further leaflets: I had generally just to touch the leaflets, for even this caused the spheres to adhere better.—

Sept 9th. 8° 30' on 2 or 3 of the sponged leaves the hairs brown giving a brown aspect to spots where water has lain occasionally (for the spots have sometimes been dry), but I suspect leaf laminæ themselves slightly browned.—

Investigate these browned Hairs compared with others on the sponged leaves.

Sept. 12th. one of the leaves not sponged, has a conspicuous yellowish brown – oval mark of considerable size, (so not due to concentration of light where water lay & I believe another yellowish.

I gathered cut off part of leaf which had been sponged, the unicellular hairs are brown & plainly different from those on other parts of the leaf on which water not rested, but which had been sponged. The veins also were brown, but this was due to the epidermis or immediately underling tissue being brown & decayed: some of the tissue in other parts was pulpy & more or less brown to some little depth. the brown marks are chiefly round the margin, as if where water & air met suffered most.

(over)

[106v]

Sept 15. unfortunately instead of leaving the 2 leaves attached I cut them off & examined them.

Leaf one. — not sponged side (1) leaflet with large yellowish mark & more transparent, 2d leaflet mentioned on the 12th with very defined yellow mark & more transparent & many brown specks (& 2d mark faint yellow— 3d leaflet with faint yellowish mark & transparent yet distinct — sponged side – (1) leaflet examined on the 12th certainly injured with spaces yellowish & pale brown specks — 3d leaflet less injured, yet spaces more transparent

Second leaf & rather older: not sponged side (1) leaflet space pale yellowish & more transparent 2d do in slighter degrees — 3d with only a trace & doubtful— (perhaps water did not always rest on same place): sponged side 1' leaflet faint yellowish spaces 2d of 3 leaflets do – 3d do with brownish specks This leaflet leaf not older, not so much injured as last, yet clearly injured.—

In the worst injured spots places, examined with high power; the brown speck are in epidermis— one whole space a very large proportion of chlorophyll has disappeared, which makes the spaces more transparent & what is left is generally of a yellower tint instead of brilliant green.— The browning of veins seems due to overlying tissue.

[107]

Sept. 9th 1877

Mimosa albida

What cd I have meant — A drop of S. Ether from a height, (leaf not touched) instantly removes silver, as does Turpentine & creosote.— leaf afterwards well wetted & water not easily removed.—