[194 and 195]

Little Bottle Gourd circumnutation & sleep of Cotyledons.

July 11th ─ 14 7° 35' am to 9° 5' am 14th 1878

Fig. 20 A

Fig. ? Lagenaria vulgaris

Circumnutation of Cotyled, 1 1/2 inch in length

Tip of Cotyledon 4 3/4 from the vertical glass, on which movements traced.

Fig 20A Little Bottle Gourd

Lagenaria vulgaris

Lagenaria vulgaris

(Scale 1/3 with Lettering This will be difficult)

Circumnutation of a well developed Cot (1 1/2 inch in length), hypocoty being the same, traced on vertical glass apex of Cot. only 4 3/4 inch from the vertical glass on when [illeg] trace made from 7° 25 am July 11 to 9° 5' am 14th

[195v]

38

Introduction

the anthers and stigma are not simultaneously mature; for I did not always attend carefully enough to this point. But even in this case of dichogamous plants, as the flowers were protected from wind, rain and the access of insects, any pollen from a distinct plant placed by me on the stigmatic surface whilst immature would generally have remained there until it the stigma was mature, or might would afterwards reach have fallen on it; and the flowers would then have been crossed as was intended. Nevertheless it is highly probable that self-fertilised seedlings have sometimes thus from this cause got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.

Errors arising from the two causes just named, and from others, ─ such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error, ─ the sickness or unperceived injury of any of the plants, ─ will

[Cross and self fertilisation, pp. 23-4: "As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.
Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck."]