105

1878 Herbaceous Cotton

15th 8º 30' all unhealthy— thrown away except 1 (5º 30') with sqr & shell-lac. refixed in damp air on Table

July 13th 9º 15' attached 4 bits of covering glass to 4 radicles with 14' 9º refixed 2 lost sqr. & added 2 fresh seeds— sand-paper & asphalte' asphalt or Balsam of Condc. & filled jar with water & placed in cellar near ice-box— temp 58º F. 14th 8º had not grown— sqr dropped off 2— Placed on sand Bath

 

(July 13th 9º 15' fixed sq. of sand-paper with Asphalte or Caud. Balsam Radicles immersed in water filled up jar with water & stood on sand-Bath. temp 70º—75º 68º—77º Room 62º—63º "Second jar)

(1) 4º 30' perhaps little curved from sq no effect (14th 8º 30' no clear effect sq washed off; refixed card & Asphalte (14' 4º 45' refixed glass & asphalte.) (15th. 8º 10 sq. with shell-lac.. in a (16th inclined from square but is whole radicle inclined not trustworthy

(2) 4º 30' no effect (14th 8º 30 has not grown)

(3) 4º 30 curved from sq (14th 8º 30 do & from seed)

(4) 4º 30 no effect (14th 8º 30 curved laterally from sq.

(5) 4º 30 no effect (14th 8º 30' curved at 50º from sqr.

[sketch]

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Cotton

(6) 13' 4º 45' glass sqr card fixed (14th 8º 30 : 4º 45 completely Hooked sqr. dropped off

(7) like (6) glass fixed (14' 8º 30'  

(8) like (7) (14th 8º. 30 sqr dropped off; refixed card & asphalt.) (dropped) 4º 45' refix (15th 8º 20 sq. with shell-lac (16.th 8º no effect)

(9) like (7) (14' 8º 30 no effect. sq. not touching apex) (14' 4º 45' refixed glass & asphalte. dropped off (15th sqr with shell-lac.) (16th 8º no effect)

(10) like (7) 14' 8º 30' sq. dropped off refixed card & asphalte — refixed glass & asphalte 15' sq. with shell lac (16th 8º. no effect

15th 8º a.m. I fond the square dropped off almost all many refixd as above — threw away 3 — Kept on sand-bath in damp air— 4º. 45 look yellowish & miserable. (16th dying)

Summary— in 5 cases the sq. caused curvature under water & in 5 cases did not act, but some of the latter were poor miserable specimens.—

106

July 14th 8º 45' a.m— on sand-Bath sqr of sand Paper & Asphalte — Jar with wooden top Cotton

Radicle immersed in water (Jar make B)

(1) straight & long 4º 45 apex bent at rt angle (15. 8º 30 now going geotropically with basal part to S. 

(2) str. a little inclined to right sq in opposition— (4º 45 no effect ) now inclined greatly to S (16th 8º a.m tip of radicle now above seed, but sq. rather lateral 

(3) str. very slightly inclined to Rt & from S (4º 45 no effect) (15 8º 30' do, but now greatly inclined to S.

(4) str. rather short inclined to left sq. in opposition— (—4º 45 no effect) (15th 8º. 30' do

(5) short greatly inclined to left sq. so as to increase inclination (4º 45' I think has acted (15 8º 30' ??) (16th yet I think has acted

(6) short inclined to left sq. in opposition to mark (4º 45 dropped off put on glass so as to make to bend from gravity (15th. 8º 30' no effect)

(7) long & straight (4º 45 no effect) 15' 8º 30' do

(8) long & straight (4º 45' no effect) (15th 8º 30' dropped off —refixd with shell lac bit of glass 16th 8º a.m. no effect

(9) short str. inclined to left sqr so as to increase inclination (4º 45 no effect) — 15th 8º 30 do)

(15th 8º 30' placed jar on sand Bath damp air: 4º 45 look very poorly.

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Cotton In Water

Summary in 1 acted, in 2 doubtfully acted in 6 did not act,. 1

General Summary of 2 papers — 19 radicles tried under water with sq. of sand-paper & bits of thin glass cemented to roots — in only 6 acted plainly in 2 doubtfully & 11 not at all, but some of the latter were poor unhealthy radicle which did not grow & in one or 2 cases squ. objects cd be seen not to touch apex— But results not conclusive nevertheless— I think I may say sometime act— It is clear that roots will keep healthy for very short time treated unnaturally as done in all cases.

107

June 20' 1879    Gossypium herbaceum— Radicles— dry germinated in damp. peat. — on chimney-piece.— apex touched only once on one side with dry caustic— at 7º. 50' a.m. Suspended close over water & well splashed a few minutes after caustic. All with caustic laterally

(1) caustic (21st 8º has now grown upside down with no relation to black mark, which is hardly 1/2 mm in length O

(2) Caustic 1º P.m I think a little bent from black mark(?) 4º 30' do (21st 8º a.m curved from black mark 2/3. of mm.

(3) caustic. 1º P.m  distinctly bent from mark. 4º 30' curvature extends down for 9 m.m— after the 8th 25 / 9 mm 25º from vertical (21st 8º curved wildly down toward cork now with no apparent relation to mark

(4) Caustic 1º P.m distinctly bent from mark 4º 30' 25º from perpendicular. — curvature 6 mm for 6 mm (21st 8º still more curved from mark — 55º from perpendicular.— mark about 2/3 m.m.) X

(5) Caustic 1º P.m distinctly bent from mark; 4. 30' has become perpendicular (21st 8º am slightly curve from mark)

(6) Caustic 1º P.m distinctly bent from mark: 4º 30, 15º from perpendicular (21' 8º 45º from perpendicular — mark about 2/3 m.m) X

(7) Caustic 4º 30' straight —(21' 8º a m black mark very minute— Sachs curvature strong no relation to mark)   O

(8) caustic 1º P.m. 7 mm distinctly bent from Caustic 4º 30', 65º from perpendicular curvature 7 mm. —(21st 8º a.m— Horizontal, but no relation to mark X

21' 8º a.m The radicles have now grown wildly— they now form a great contrast with the 7 control specimens, only one of which has grown wildly, viz up towards & touching cork

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Control specimen at same time suspended

(1)   4º 30' P.m— all 7 straight & perpendicularly down, except no 10, which is very long radicle & this is straight but has grown inclined—

(2)

(3)

(4)

(5)

(6)

(7)

Conclusion. out of the 8 with caustic 4 distinctly bent, after 8º & even after 5º, & more so after 24º the movement case — 2 slightly & 2 not at all In the latter the black mark extremely minute great contrast with the 7 controls — Black mark in no case 1 m.m in length, curvature extend from 9 m to 6 mm.— After 24º the radicles had grown wildly in all sorts of directions.