[109]

Nov. 6th

sqr fixed 9º 30'

Spanish Chesnut

Jar B

Jar C Horse-Chesnuts Nov 8º 9º 30' a.m strips of Copper— also at 9º 15' on some Spanish-Chesnuts

(Nov. 9th In Big 7 Spanish C. tried repeatedly with sq. of sand-paper no effect.— Bit of lead [sketch] this caused of course deflection, & 8º 15' put square of card on deflected portion to see if this will increase curvature: also put same lead cap on another radicle.

(Nov. 10th I may say that I tried made about a dozen trials with sand-paper & shell-lac on radicle of Horse & Spanish chesnut, but none were affected. The radicles are extremely stiff & may well be that they require a much more powerful stimulus than a bit of card on tip.— I think so because I fixed curved bits of thin metal to base & obliquely across tips, & these became bent [illeg] curved towards their tips in nearly the same manner as in case of bean: it may of course been simply mechanical obstruction, but the appearance rather indicated curvature from unequal growth on the 2 sides)

[109v]

Square of Sand-paper (Spanish Chesnut)

shell-lacced to apex— Kept in dark — pinned to roof in Study.—

(1) Oct 27' 8º 45' (29th 8º dropped off refixed, no effect

(2) Oct. 27' 8º. 45'. (29th removed no effect & refixed) 30th 8º I think acted a little

(3) Oct 27' 8º 45— very short 1 young (29th. 8º slight curvature from sqr.) 30th 8º I think so.

(4) Oct 27' 8º 45, 2 secondary radicles, end of main radicle having been killed by some accident. Oct 29' 8º. 30 very short

(5) Oct 29th 8º 30' very short R.

(6) Oct 29' 8º 30' very short

(7) Oct 29' 8º. 30 very short

No curvature in any case to be trusted or with Horse-chesnuts

[110]

Nov. 15' 1878 Radicles of Horse-chesnut— Great Jar

8 Radicles some with bit of glass & shell-lac— others with ca sand-paper & gum.

(3 of radicles, marked internally with pin, had grown to considerable length in water) also some with sand-paper & shell

Nov. 15. 4 radicles of Horse-chesnut which had grown in water to between 1 1/2 & 2 3/4 in length, had were then kept in damp air (—Temp about 65º-F) with square of sand-paper fastened with shell lac to tips, but no curvature caused after 48º—

Jar B— all 4 germinated grown in water with sand-paper & gum. 8º 50' a.m. Nov. 15th

Nov 17' of the 12 radicles in this paper the card having been replaced, why they fell off none produced the least effect

[111]

Nov. 17th 1878

12º.

Radicle of Horse-chesnut.

Radicles old & not good ones Lunar Caustic

The 12 radicles which had been tried with glass & card with gum & shell lac, & which had not acted were washed & dried, & each touch 2 or 3 times on one side with stick, just damped of Lunar Caustic— Most of Roots rather old & long & not very healthy— after being touched their distal or lower portion immersed in water— Temp about 66º F— in dark.— Temp 66º F.

Nov. 18' 8º a.m In Big Jar the tips of the 8 radicles blackened all round— & no curvature

In the small jar the 4 very long radicles which had grown in water now all have their extreme tips turned towards blackened side.

(N.B I ought to have rubbed radicles with dry nitre of S stick & only once or twice.)

Nov. 19th 8º a.m— in Big jar tips all utterly killed & radicles, contorted.

à (A radicle 2.2 in length was actually .3 of inch in thickness — to show strength)