99

(p1

Gossypium herbaceum (from Thompson)

Jun 14 5° P.m 13 radicles, some long, some short pinned to cork lid & kept on chimney piece.— examined 15' 8° 30' a.m

(15 4° 40' P.m Cork dropped off & fell into water & washed off all the squares!!! but what then took place I know not)

(1) broken

(2) 15. 8. 30 not affected. ⨀

(3) bowed from card large piece, but to Sachs   ?

(4) not affected   O

(5) (2 pins) bowed laterally from card ✔

(6) bowed. a little laterally (4°. 45' bowed a good deal laterally, but card washed off ✔

(7) not affected O

(8) card dropped off (15 9° am put on big card)

99v

 (9) not affected (15 9° put on big card but not well  O

(10) (Big Pin) bowed to card   O

(11) slightly bowed from card (4° 45 considerably bowed) ✔

(12) straight (15' 9° put on big card but not well)   O

(13) bowed from card, but to Sachs— 4° 45 greatly bowed ? (15' 9° a.m. put on big square up to 23°.)

 6/13 appear to have been slightly affected after 15° 30' 

Result

100

(2

Another jar small Cotton

(11) (marked with pin) June 29th 9° a.m R straight fixed sq 4° 40' bent from squ. but still straight July 1'. 8° r. horizontal, whether due to sqr I cannot say   ?

(12) 29' 9° a.m r. straight R— — fixed sq — 4°. 40' no effect) (30. 8.20' do looks unhealthy.) 0 July 1. dying

(13) 29' Big Pin 9° a.m R strait fixed sqr 4° 40' no effect — (30th. 8° 30 quite straight. O

(14) 29' Biggish pin 9° a.m R straight fixed sqr 4° 40 no effect. (30th 8° 20 straight. but sq has slipped off O

(15) 29° Biggish pn 4° 45 fixed sqr straight radicle short —(30th 8° 20 tip curved to sq. a little.   O

(16) 29' small pin. 4° 45 fixed sqr. radicle straight not short & short —(30' 8° 20 no effect O

(17) 29' small pin 4° 45 fixed squ — radicle straight — (30. 8° 20'' a little bent from sq. &; 30'' 4° 30' distinctly bent from sqr. (July 1' 8° a m .at right angle ✔

 July 1  st 8° a the whole lot yellow & dreadfully unhealthy I cannot tell why

100v

(2A

Cotton Herbaceous

(1) (28th 8° 15' sq fixed 5° P.m curved from sqr but slipped off refixed (29th. 8° 45 again slipped off & radicle look brown throw away) ?

(2) 28' 8° 15 sq. fixed— 5° Pm little curved from sq. (29th 8°  45' am— apex at right angles. but sqr loose 4° 45 sq dropped off  ✔

(3) 28' 8° 15 sqr fixed 5° P.m straight — (29th 8° 45 little curved from sqr— put fresh spe. & refixed fixed new sqr 29th 4° 30 no effect) (30th 8° 10' inclined from sqr. but in direction of Sachs & terminal portion straight.

(July 1' 7° 30' a.m The radicle is now quite Horizontal which must be attributed to the sq. on the lower side) ✔

(4) 28' 8° 15 sqr fixed 5° Pm straight (29th 8° 45 no effect, but then there is shell-lac all round)

(29' 9° put fresh spec. radicle straight 4° 30' no effect) (30th 8° 10' sq. dropped off, refixed one O

(July 1st 7° 40' a.m — decidedly bent to one side & to S. in accordance with position of sq.)   ✔

(5) 28' 5° 10' fixed sqr quite straight — 29th 8° 45 greatly curved from sq but chiefly toward S &  a little to one side  ?

(6) 28' 5°. 10 fixed sq quite straight but very short (29' 8° 45 quite straight but sqr on summit nearly at right angles to radicle, 4° 30 quite straight O

(8) 29th  9° a.m. r. straight. fixed sq 4° 30' no effect (30th 8° 15 sq. dropped off, refixed sq.) July 1. 7° 40' no effect x) but all lower part of r. brown O

(9) 29' 9° a.m r. straight fixed sqr 4° 30' no effect (30th 8° 15 perhaps a little curved from sqr). July 1' 7° 40' terminal portion decidedly bent from sq.) ✔

over

101

(3

Herbaceous Cotton— Radicles

June 30th 8° 40' a.m. fixed squ. of sand-paper & glass on 13 radicles all straight, excepting 1 (with free oblique pin by base) & this has basal part curved— day cooler temp. Temp on study Table 20° to 20 1/2 C.

(These were looked at 4° 30' P.m & only no 4' affected at all)

(1) (1 Big Pin) July 1 8° a.m. no effect   O

(2) 30' 4° 40' fixed bit of glass   July 1' 8° am. no effect yellow & unhealthy O

(3) July 1. 8° am squ dropped off unhealthy

(4) cinder. 30th 4° 35' a little curved from do ✔ slightly July 1' 8° cinder dropped off, straight

(5) (2 Big Pins) July 1' 8° a m no effect   O

 (6) July 1' 8° a m no effect  O

(7) July 1' 8' a.m no effect   O

(8) July 1 8° a.m no effect   O

over

101v

3A

Herbaceous Cotton

(9.) July 1' 8° a.m unhealthy no effect O

(10) (3 Big Pins) July 1. 8° a m no effect ? but as much curved from S. may have curled ?

(11) July 1' 5° am yellow unhealthy— much curved from S. & card ✔

(12) July 1. 8° a m sq dropped off straight   O

(13) July 1' 8° a.m no effect. O (July 1' 5°. '30 P.m tip now curved to sq.

Five Six of these were left viz (1) 10 (5), (6) (7) (10) & ( n 13) at 5°. 30' P.m. on July 1'. & I am nearly sure hung down more or less perpendicularly & now 5 of them are extended almost horizontally, being bent close  to the seed itself & not at the tip— they are rigid but do not look healthy— This is an extraordinary fact.— Have they been attracted to the damp cork, but if so why has not tip bent? July 3d 8° 30' a.m the 6th radicle has now turned up so as to be horizontal— The other are limpid — I have little doubt that this movement is precursor of death, but why it occurs I know not.—

102

(4

A   Cotton— July 5' 5° P.m. sq. fixed— in Kitchen

(July 6th 11° a.m. Kept on Study Table— roots do not look well) 4° 50' no effect roots look very bad.

(1) short st geotropic (July 6th 11° no effect. on any)

July 7' 7° 45' a .m no effect, all look unhealthy) Thrown away

(2) short straight geotrop

In Kitchen on July 5th general temp was from 23° to 26° C. & this seemed m too hot, but then heat was not applied below, & so radicles may have become too dry.)

(3) like above

(4) like above

(On July 6th on my study Table Temp. from 18 1/2 to 19 1/2, but the radicles did not recover.)

(5) straight inclined over seed

6) straight geotropic

(7) inclined little over seed & to right.

(over

103

(p 5

Cotton

(9) 7th 8°— no effect sqr. slipped — refixed. (5° no effect)

(10) 2 Big Pins 7th 8° curved to S. fixed sq (5° no effect

(11) 7' 8° lower part crooked fixed sqr (5° completely Hooked but sq. dropped off

(12) 7' 8° fixed sq (5° no effect)

(13) 7' 8° fixed sq (5° no effect)

(14) (Central) 7th 8° fixed sq (5° no effect)

July 7' 5° P.m the bases of all radicles yellowish-brown.

All the above radicles straight & geotropic except 2 or 3 somewhat inclined to S. & one a little crooked in basal part above alluded to

(July 8th 8°. 30' I can see no evidence of sqr. having acted.—)

103v

(p5

Jar B      Cotton   July 6th 11° 30' sqr fixed on; Kept on Study Table —(Temp July 6th during day 18 1/2 to 19 1/2. C.)

(1) 5° P.m no effect except, perhaps in one case. ☞ July 7th 8° a.m put on Sand Bath

(1) 7th 8° no effect 8° fixed sqr 5° P.m curved to S ?

(2) 7th 8° a.m a little curved from sqr (this is one noticed at 5° P.m last night) 5° P.m. much deflected but sq. dropped off ✔

(3) 7th 8° fixed sq (5° much curved to S. but sq. dropped off ?

(4) 7th 8° a. fixed sq (5° no effect   O

(5) (Big Pin) 7th 8° no effect (5° curved to S.   O

(6) 7th 8° no effect (5° do)   O

(7) 7' 8° no effect sq. slipped refixed sq (5° no effect.) O

(8) 7th 8° no effect (5°. do   O

(9)

  I had better try another lot on sand-bath with evaporation & not very hot

104

(p 6

July 7th Cotton

5° P.m fixed squ on 6 radicles which had gone in water all day & were nearly all fairly straight— & placed on warmest part of sand-Bath

July 8th 8°. 15 all 6 look yellowish & unhealthy — sq rubbed off several.— I can see no evidence of sq. having acted— 4 of them strongly inclined towards S.