(1

1878) Cassia— grew near sea-side formerly observed with sensitive Cotyledons— New lot of seeds.

Sensitiveness

July 22d Rubbed 2 cots on different plant, resting on my finger & rubbed both cots on another seedling each for 30" with little twig — Sometimes same Cot. & sometimes opposite one rose, perhaps depends on pulvinus being rubbed, for this seems the most sensitive part, for I pinced pricked pulvinus of 1 seedlings & Cot become quite vertical.; as did another Cot. of another plant very warm cloudy day in Hot House.

I then pricked for 30" with pin, resting on finger a Cot, but did not touch pulvinus, & the opposite Cot. rose.

I [pinched] pulvinus & this Cot rose & stood quite vertical, I succeeded in no instance in making both Cots close.—

(2

In Study. temp only 20° C.

Pricked with needle blade & pulvinus, this Cot rose greatly, but not opposite one which remained horizontal.

Pricked whole leaf but not pulvinus— pricked Cot rose considerably, but not the opposite one very little.

Pricked whole leaf but not pulvinus, opposite one which was horizontal rose greatly & not the pricked one.— (Could I have touched opposite one on under side with end of finger?]

Pricked once pulvinus of 1 Cot. & both Cots rose!

(July 28. young leaves just appearing— rubbed 1 cot, not supporting it, & it rose a little, but not the opposite one.

(Rubbed pulvinus alone of one cot. I think both rose a little.)

Pricked only pulvinus of 1 Cot, in 3' pricked Cot. rose & in 6' additional minute stood vertical highly inclined — opposite Cot, not affected. Then pricked & rubbed the already raised cot, supporting it on my finger, but not touching near side of pulvinus on opposite Cot. & the opposite Cot, not affected.— Rubbed lower side of l same pulvinus & upper part of Hypocoty, opposite Cot. not affected. (over

[2v]

July 28' continued.

(Rubbed & pricked a cot, already raised by light, but not pulvinus, resting on finger, but not touching under side of pulvinus, &: in 6' the pricked cot was vertical, opposite one not affected.

(Repeated experiment on another seedling, with same results

Minute drops of nitric a. on pulvinus & it extended to opposite one— both cots rose so quickly that cd be seen to rise, & afterwards almost immediately began falling — surfaces brown & killed.—

(The experiments make it very doubtful whether effect transmitted to opposite Cot.)