[50]

Sept 4th 1878

Cassia "Large beautiful unique tree".

Effects of Touching & Heliotropism

Cots. rather elongated. — Hot day. Observation in Hot-House.—

A. Cot. 17° above horizon, rubbed for 1' lamina & pulvinus with pointed stick: in 26' stood 48° above Horizon so had risen 31°

Used

Sensitiveness

A second Cot. stood 27° above horizon rubbed in same way lamina alone; not touching pulvinus— & after 26' stood 35° above Horizon so had risen only 8°

(True Leaves sleep in usual fashion)

→ Effects of light Light

Day moderately bright & hot— pot brought from Hot House & placed at 12° 26 on my middle study Table; at 1° 2' ie in 36', all the Cots, completely closed, except those of the seedling of which I had rubbed the pulvinus, & this was not much shut.— At 2° 15' (ie in 1° 13' all the cots were beginning to open except the same ones of which I had rubbed pulvinus & which did not close so quick as others, & this now (ie at 2°. 15') was completely closed— So that stimulus from touch retarded action of darkness. No

(over)

[in margin:] Worth trying again

[50v]

Sept. 5th I rubbed pulvinus of 3 cots. on 3 seedlings, & 2 of them were much raised after 25', & then placed them on Study Table— The day was dark in Hothouse, so I left the 2 pots in Box open at top on study Table, & after 50' many were completely closed, including 2 which had had their pulvinus rubbed, so this does not delay the action of darkness.—

[51]

Sept. 4' 1878

Cassia "sea-side'

S. Ether

Cot. with glass filament under skylight traced on vertical glass— fr 8° 50' to 12'. 24' circumnutating grandly, then placed under the large bell-glass 30 minim of Sulphuric ether in watch-glass— [sketch]

In 14' little risen; in 11' more time a little sinking; in 15' still sinking little; in 26' sinking— In the whole 1° 6' circumnutated very little compared with what it had done before.— — I then scratched pulvinus of this & other cot for 1' & put on bell-glass, with ether; & both at cots rose greatly, put on bell-glass with more ether, & then began to fall— took off bell-glass at 2° 21 & cot. went on circumnutating as well as ever— As far as this one experiment goes vapor of S. Ether has more effect on circumnutation than on sensitiveness.

[52]

Cassia. S. Brazil growing near coast F. Muller.—

Sept 15 9° 30' Cotyledons turn up & sleep at night so as to stand vertically.

shook one, tapping with very fine twig, cotyledons up & down for 3' in a few minutes the 2 stood at 90°, instead of 180° together. — in a very short time recovered horizontal position.— No bloom, but surface repellent. Used

A drop of water on cots. does not cause movement, but syringe with very fine syringing does.

Tapping one cot. causes this one to move.

Sept. 15th put 3 drops of water on 3 Cots no effect.

— 8°. 52' tapped for 1' 2 cots. which stood at right angles to stem: one rose only a little; the other rose in 9' to angle 117° & on 7' or 8' additional minutes to 127°, so had risen 37°— opposite cot, hardly at all affected. In 26' from tapping horizontal again.

Another pair of Cot. which was at ∟ to stem was tapped gently, rather more down than up, & rose in 11' 23°

[53]

Cassia— "large beautiful tree"

Sept 20th. Cotyledon have grown immensely & now stand vertically 7° P.m, asleep & was horizontal during day.— yet some of seedlings have 2 true leaves of small size.— — I suppose I have not when I observed them sleep formerly Sept 4th & sensitiveness to light, & this will show for how long they continue sleeping.—

The true leaves sleep in normal fashion

[in margin:] It may be in light Chapter

[54]

Oct 10 1878— Cassia — "sea-side".— (C. Tosa)

Having Having noticed in my experiments yesterday that some seedlings which had been exposed to Heliotropism, before feeble light before in N.E window in on the 7th — & kept dark at during all night, opened in the morning 8th, though still kept in dark, but began to close about 2° or 3° P.m.— (6° or 8°)

Therefore another pot of same seedlings of same plant was kept in my study on Oct 9th. with Cots open & at about rt angles to each other, & at 10° a.m put behind Book-Screen with only very obscure light, in 1° the Cots closed almost completely; then put before Study window, & 1° opened again.— At night 10° P.m quite shut & then placed in complete darkness.— But next morning at 7° a.m 10th were well open ie Cots at right angles.— They so continued till 11° 30' (ie for 4° 30' open) when they began slowly to close; by 1° 30' considerably shut: by 2° 30' almost shut, but not so completely as at during last night.

(over)

[54v]

This is good case of imperfect habit, & also shows the great power of previous light, & darkness; for in this case opened & kept opened for hours, having previously been in darkness— whereas if had opened in light & then put into only obscure light wd have closed in 1° or less time.—

Continued)

at 5° 49' P.m no change: at 10° 15'. P.m only a little more shut; not so completely as last night—

Oct 11th 7° a.m. (next morning) again moderately open — again inheritance.