[10]

Aug 23d. Southampton — Examined 6 flowers of short-styled all had pistil bent, some at completely right-angle; some only a little. The lower anthers are often slightly graduated & the upper green ones; at other time quite equal in length, so that this point also variable.— Colour of upper green anthers certainly variable in darkness; quite pale greenish yellow in one specimen.— I may say that longer stamens sub-equal in length, slightly graduated.— Longest stigma roughest — & gradation with marked exception

The length of papillæ on stigma variable; I have seen great difference in state of that of mid-styled— I think generally that of long-styled, which seem always to have biggest stigma, has longest papillæ— but I compared one mid- & long-styled equally rough— Though 3 forms exist they are all in a far more fluctuating state than in Primula; so I believe is pollen.— (over)

[10v]

They are just in that state that natural selection could do much, if profitable —

Colour, size of pollen-grains — rough gradation of length of stamen — bending of pistil & roughness of stigma.—

I have been examining some 6 Hive-Bees caught sucking; 4 had been pollen-collectors, & 2 of them seemed to have collected only yellow & 2 only green pollen!!! The a yellow & green pollen seem dusted all over body & forehead; but I think on chin under proboscis only yellow pollen, no doubt from short stamens. The pollen so disturbed by catching Bees, hard to say; but I think it stands to reason, must adhere to different parts of body. —

pollen of sh. st. of long-styled longer than of sh st of mid-styled??

[11]

Aug. 26th— Southampton— Watched Lythrum saw many Hive-B — many Rhingya & Syrphidae & 1 Humble. It was clear that long stigma & corresponding anthers struck body between 2 hinder pairs of legs— Stigma of mid-styled on under side of thorax & front legs —

Stigma of short-styled must rub against proboscis itself & perhaps chin; The Bees drove front of head into calyx.—

Most of Bees were sucking.

[in margin:] Variability

I examined 5 more Bees; plenty of green pollen on inner side of 2 hinder pairs; not so much yellow, as I shd have expected between front legs, still some had much — I must leave case vague.—

[12]

These bees were all sucking, but 3 of them had also collected pollen; & apparently all green; but under microscope the grains were unequal— Probably greater length of green in the 2 forms would account for collecting that in preference — yet odd how completely yellow the pollen was on 2 of Bees collected yesterday.—

Aug 27th It is all nonsense about pollen-gatherers, for I find pollen from other plants — now that 2 Bees are dry, I can see plainly green pollen, betwen 2 pairs of hinder legs & on legs themselves, & plenty of yellow between two front legs, & yellow pollen on hairs on each side of depression where proboscis is lodged, which no doubt come from the short stamens

[12v]

Aug 28. William by drawing could not show that pollen of long st. of long-styled was larger than pollen of short-stamen — but on comparing dry & wet & dry again we all agreed that if there was any difference, (which seemed to me the case,) then that pollen of longer stamens was a shade larger, as I concluded at Down & as William found to be the case formerly —

Certainly pollen of corresponding heights of all 3 are same size — I have now compared, (without knowing which was which) pollen of shorter sta of short-styled with that of shorter stamen of mid-styled, & as ought to be, if there was any difference the former was largest. So say subject to some variability. I almost invariably concluded that middle stamen had larger largest pollen than that of 2 sets of shortest stamen —

[in margin:] I have looked at another long-styled showed no difference in pollen of the 2 sets of anthers.