[F10]

Cyclamen Persicum in 7' to 1000 from 3˚ 11' to 10˚ 13th = 43 hr.

At base of some cells solid mass of dark green chlorophyll — In other places grain on walls can be seen to be confluent — All gradation between heap of grains & probably confluent 1/2 moon at base of cell, often cling with alcohol — 1/2 moons at first intense green —

Jan 14th 11˚ a.m left immersed in same sol. — most of cells now filled with pulpy green matter still partially retaining outline of much swollen Ch gr. — in other part homogenous green pulp.— Now where the 1/2 moon, all have swelled & disappeared — Now cells mere vascular bundles — spherical spiral & oddly shaped agg mass globules of pale blue; such as occur in roots!

[Darwin, C. R. 1882. The action of carbonate of ammonia on the roots of certain plants. [Read 16 March.] Journal of the Linnean Society of London (Botany) 19: 239-261, p. 255: "With some thick fleshy roots, after an immersion for 42 hours (and thick roots require a long immersion for the full effect to be produced) the green granules in the parenchyma-cells had become completely confluent, and now formed spheres of transparent green matter of considerable size.
The granules are not dissolved, nor is their colour discharged by sulphuric ether. Acetic acid instantly changes the green into a dull orange tint. The granules are not dissolved by alcohol. Their precipitation by the ammonia solution seems to depend on the life of the cell; for some transverse sections were examined and found colourless, as well as destitute of granules. They were then irrigated with a solution of 7 to 1000, and reexamined after 22 hours; and only a very few cells in two out of the five sections showed any trace of colour, which, oddly enough, was blue instead of green."]