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1881

Aggregation of Chlorophyll. C. of Ammonia.

Put end of Lilac-leaf in sol. of 2 to 1000 for 3˚ 15'; also after examining fresh spec. in slices added drop under cover-glass of 4 to 1000 & examined next morning, but I could see no aggregation of chlorophyll granules.

Fuchsia a leaf was left immersed in sol. of 2 to 1000 for 25hrs, but I could see no aggregation of Chlorophyll granules in comparison with slices of [fresh]-leaves.— Perhaps no absorption for the extreme cut petiole was blackened, & if there had been absorption, the whole leaf probably wd have been blackened.

Robinia pseudo-acacia. Aug 10th Leaf absorbed by petiole during about 16 h. same sol. of 4 to 1000, & base of leaf blackened. In this section, several of palisade cells full of blackish matter which consists of fine spherical granules— an interrupted row

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of similar black cells, still blacker, but apparently of same nature on lower side close over epidermis. In middle of leaf cells with fine green chlorophyll granules & some cells filled with homogeneous greenish matter, & such cells were present in section of leaves which had been cut.—

I have now examined leaf which had absorbed sol of only 2 to 1000 during 17 1/2 hours — no cells blackened, but many palisades with their longitudinal lateral walls internally darkened, apparently the protoplasm on walls thus blackened. —

Perhaps cells only killed. —

Aug 12' Specimen of do left for 24 h in the sol. of C. of Amm 2 to 1000, but no extra effect produced, except a little browner.—

The cells with granular matter, a little above the tip still granular.

Left a specimen for 2˚ 20' in alcohol & water (about 1/2 & 1/2.) root rendered very opake — no distinct aggregation, but rows of cells, generally alternated,