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Fumaria parviflora (?) in C. of amm. 7 to 1000 from Oct I 12˚ 30' to 8˚ 30' a.m on 3d.

No aggregation.— but contents of cells more granular apparently from equal immersion in 4 to 1000 for same time.—

Mirabilis Jalapa — Cut leaf placed in sol. of C. of am 4 to 1000 at at 4˚ 30' P.m Oct 2d, 16 1/2h sliced Oct 3d 9˚ a.m.— no agg. but Ch. grain form horse-shoe in some of cells.

The same cut placed at 4˚ 30' P.m Oct 2d in sol. 7 to 1000 & sliced Oct 3d 10˚ 30' a.m, contents of pallisade little affected, but in parenchyma below, the grain form horse-shoe or perfect rings (which when seen extending wd look like green balls) or horse-shoe shaped masses on one-side.

Grain continues confluent, sometimes distinct, but adhering so firmly that a curved portion projected from one cut cell. — In slices in which no grain could be

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Mirabilis

seen (alcohol not having been used) after Acetic A. had been added the grain almost or even quite disappeared for they were rendered so transparent & particles of starch cd be seen within them.

It is evident that A.A. acts powerfully on the grain after C. of am., & this agrees with disappearance of the spheres in Geranium.—

In lower epidermis cells minute brownish spheres; after acetic acid particles cd be seen in lower epidermis cells, which I think became blue with the iodine; but too minute to be sure. This makes me believe that there are chlorophyll grains in lower epidermis.—

Oct 4' F. made slices of fresh leaf, & I see numerous particles in the epidermis cells & some form little spheres.

I reexamined above leaf, immersed in C. of amm. 7 to 1000 from Oct 2d 4˚ 30' P.m now to Oct 4th 9˚ 30' a.m, & there was

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Mirabilis

no aggregation. the Ch. grain were often confluent or touching forming horse-shoe or complete rings. One slice was cleaned with alcohol, & now clear that gr. were completely confluent, forming horse-shoe. When Acetic A. was added, the horse-shoe became so transparent , that outline cd hardly be distinguished; there was merely a space more transparent than elsewhere in cell, & now innumerable particles, no doubt starch, were rendered plainly visible

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1881. Oct 9'

Nepenthes sileni garden Hybrid)

Slice of fresh leaf in glycerine for 24 hrs, outer & inner epidermis cells empty & transparent. — no agg. in the internal glands. In cells beneath epidermis plenty of green Ch. grain, even where surface was red. —

A slice of C. of am. (7 to 1000) from 8˚ 10 a.m on 7th to 10˚ a.m. on 8th, then examined & reexamined after 24 hr in glycerine. Interior epidermis cells filled with fine granular matter, but no aggregated spheres, except in the glands, which contained plenty of dark brown or very orange or very pale, true agg. spheres. In parenchyma Ch. grains generally somewhat disintegrated, but often perfect, no trace of aggregation. In exterior epidermis cells, everywhere splendid agg. spheres more or less confluent of various size of dark brown, orange, purple, pale or greenish colours — Cells full of such spheres.—

Another spec in C. of am from 8˚ a.m on 7th to 9˚ on 9th cells of all kinds in same state as above.

In fresh slice from green part of pitcher no ch. grain in exterior epidermis.—

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Oct 12

(Tropæolum)

Tropæolum majus — Slice of fresh leaf — innumerable ch. grains can be seen even when slice rather thick in all the cells, but plainest in cells below palissades.

(Part of same leaf placed in sol of 7 to 1000 on Oct 11th 8˚ a.m & examined after 24˚ no aggregation, but as far as I cd see palissades & other cells full of green granular matter & no true ag. visible A multitude of round starch grain coloured blue by iodide.

Oct 13th at 9˚ a.m reexamined slices — no agg. ch. grain all broken down, & evidently many minute particles of blue (after iodide) starch in the midrib of the green granular matter. This case of allied plant makes me doubt whether spheres in Pelargonium ever truly aggregated masses.

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Oxalis

Oxalis Bowii (so-called) in sol. of 7 to 1000 from (Oct 11' 8˚. a.m to 11˚ on 12th 27˚, every Chl. innumerable, perfectly distinct— perhaps has not absorbed any ammonia— can be seen by [grasping] even when slices rather thick.—

Oct 13' 9˚ a.m. — reexamined slices. Chlor. grain still in most places perfect— no trace of aggregation

Nicotiana tabacum in sol. of 7 to 1000 from Oct 12th 8˚ a.m to 9˚ a.m. 13th, no vestige of aggregation— Ch. grains all perfect. —

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Oct 15' Cut piece of foot of Leek in sol. of 7 to 1000 & of 4 to 1000 for 24h no aggregation.— Ch. grains became finely granular.

Beet cut piece of leaf in sol of 4 to 1000 for 25˚ no aggregation— Ch. grain very perfect. — In epidermis cells fine purplish-brown, granular matter somewhat aggregated.—

Marchantia & Fern (Polypodium?) in 7 to 1000 for 24 hr. no aggregation.

Cabbage in sol. of 7 to 1000 from 12˚ 30' on 15th to 9˚ a.m 16th.— no agg, but grains seemed to me in part confluent; when cleaned by alcohol, this seemed due to the contraction of the primordial utricle & the drawing together of the embedded ch. grains.— This makes ne doubtful about other cases. (do. after 47 h. in sol 7 to 1000 no aggregation whatever, nor any confluent grain — cleaned — in alcohol. —