A

1881 Geranium Aggregation

Saxifraga sarmentosa (?) leaf put into sol. of C. of Ammonia 2 to 1000

Aug 29' 8˚ 20; examined [illeg]

August 30th, 9˚ a.m — no aggregation except in glands — Chlorophyll grains often collected in groups on one side of cell.— (over)

Geranium, similarly exposed, Aug 29th examined at 11˚ a.m, here & there grains, more or less distinctly confluent

Nicotiana do. hardly a trace of aggregation.

(Aug 31st Same leaves left in the solution: from 29th 8˚ 30 2 to 1000 50˚ examined 10˚ 45 Geranium leaf, thin sections, splendid aggregation, green balls of various sizes, in the pallisade & other cells & even in epidermis. Frank has seen & agrees like case of Drosera, exactly

Saxifraga sarmentosa 11˚ a.m — no aggregation, but the Chloro. grains are heaped together, generally at bottom of cells, in close contact & sometimes indenting each other — Purple cells now colourless —Sept 1' no change

Nicotiana, no trace or ordinary aggregation, but in parench cells the grains →

(Sept I. pulpy grain matter & grains; all in parts brownish & dead.)

[Av]

Saxifraga umbrosa (London Pride) tuft of leaves put into sol. of 4 to 1000 at 8˚ a.m. in 14th, examined on 16th 10˚ 30' a.m. — Some leaves blackened —. made sections of a still green leaf — no aggregation of Chloro. grains; in some few cells all blackened. —

(B

have united into a green horse-shoe layer filling large part of wall of cells — This layer could be here & there seem obscurely still to consist of the former grains.

Sept. 3d. Leaf of zonal Geranium in sol. of C. of Ammonia 4 to 1000, cut & left immersed for 24˚ 10' — splendid aggregation best seen in pallisade cells — also in globular heads of Hairs— no movement in masses — yet form of sphere indicate that the larger ones due to influence of smaller spheres; & the l where they are large few in same cell, too dark-coloured to have been formed by mere swelling of single cells.

Saxifraga leaf — after immersion for nearly 25 hr. in sol. 4 to 1000— Chlorophyll grain in heaps (as on former occasion) at both one end of cells, & I think grains in some case with diffused margins, as if becoming confluent.

[F25v]

Sept. 4th — Sa 10˚ 30' a.m. Same leaf of Saxifraga, after so much continued immersion — in nearly same state, but horse-shoe aggregation of Chlorophyll much darker, almost black, & in some cells the green grain plainly confluent to a certain degree.

Geranium 11˚ a.m same leaf after continued immersion, now immense green spheres in the epidermis cells above the pallisade & also some in lower epidermis — Also much aggregation in the parenchyma everywhere.

[illeg] movement of masses.