(1

Drosera Aggregation

A pale leaf placed in sol. of C. of ammonia 2 to 1000 & examined after about 24 hrs. — Whilst fresh I saw grain of r. chlorophyll in cells near base of all tentacles— after the ammonia near bases innumerable greenish oil-like spheres, but in several cells some chlorophyll granules— many of them had become spherical — In others the grains now formed [sketch] a continuous flexuous little ridge on walls. In the guard-cells of stomata many grains of chlorophyll & these had run into balls— Near bases in some of the cells merely green granular matter , instead of distinct grains — In disc of leaf near base in many part numerous green globular instead of Chlorophyll grains, Will chlorophyll again form grains?

(Aug 18' Leaves left for 20 hrs in sol. of 1 to 2000 water had glands blackened & much aggregation, but not extending to base, but no inflection!!!)

(2

Drosera Aggregation

Aug. 21. Frank made slice across disc of fresh leaf & cells of disc & of bases of tentacles, [illeg] with chlorophyll granules, in centre of which starch granules could be doubtfully seen.— Some of the cells contained green granular & transparent matter which I believe originally existed & not due to slicing??—

Other leaves which had been exposed to C. of amm for about 24 h 22 hr (4 to 1000??) were sliced by Frank & showed splendid aggregation. In cells of disc & at base an infinitude of green aggregated spheres & in most of the cells no chlorophyll granules.— This observed scores of time & thus shows that the protoplasm chlorophy aggregates. In many of the cells little ellipses which appear like starch granules, from which the surrounding protoplasm had been withdrawn — None of these ellipses in a free state could be seen in the sections of a fresh leaf. The cells with spheres or aggregated (over)

[2v]

matter chiefly in the upper & lower epidermis— The central cells in disc only occasionally with aggregated masses. — When iodine was added to the slide & had penetrated into the central cells the elliptical & some rounded grains became beautifully blue, & the green aggregated spheres yellow, which agrees with this being protoplasm. — It is clear that in immense starch grains are left quite bare.

It is certain that in Drosera the epidermis cells contain grains of chlorophyll.

(Aug 22d. A leaf had been left in C. of am. 4 to 1000, for 3 days, Killed & blackened & flaccid — aggregated spheres innumerable black, but quite distinct outlines— very little green matter left — no chlorophyll grains, but many of the ellipses — I added iodine & they instantly became fine blue i.e. those close to the surface, but those in cells in middle from section rather thick, did not change colour quickly. Effect of 7 to 1000 — [illeg]

(3

1881. Recovery Drosera Aggregation Recovery

Aug. 28' 8˚ a.m. — Plants growing on moss — large drop of 2 C. of Ammonia to 1000 on 6 leaves white Thread (Poor)

Large drops of 4 to 1000 on 6 leaves Black Threads

Aug 30' 9˚ a.m. examined by section one leaf black Thread — aggregation with colourless or pale green spheres down to very base of tentacles, & a very few here & there in disc., but others quite exceptional.

Sept 2d. 10˚ a.m drops added ever since— Those with the 2 to 1000 (5 days) quite red & hardly any aggregation.

Those with 4 to 1000 with black glands in the central parts of disc, & here much aggregation & green spheres.— May these not be green cell-sap?

(Sept 3d. 7˚ see (z) I have carefully examined several short central green tentacles middle of disc of pale red leaf to which nothing had been done & in all I can see by careful focussing flattened, very minute, grains of chlorophyll attached to cell-walls & other rounded & apparently free. If there are none of those after aggregation, the grain must aggregate. I think that there is also greenish cell-sap, but F. thinks very doubtful whether due to diffused chlorophyll I cannot say.— (over)

[3v]

(Whole plants were taken out of moss on Aug 25th at 8˚ a.m & submerged in sol. of 4 to 1000 & taken out on 26th after 24 hr — very few green spheres chlorophyll grain apparently swollen — I suspect plants killed — Planted in peat & now Aug 30th the plants leaves certainly appear all dead.)

Sept 4th I examined a leaf on which drops of 4 to 1000 had been placed on Aug 28th & had been washed clean on Sept 2d. & from which slices had been cut, but I then did not examine carefully enough for Ch. grains. — Now Sept 4th some aggregation & very pale aggregated masses were moving—in some of the cells greenish spherical free grains, with darkish centres, which I believe are chlorophyll & others, only a few elliptical lying on walls & sometimes in little heaps. I believe regeneration; but 2 short tentacles contained brownish granular matter, which I believe had been killed. —

(4

Sept 4th 48 [-] 6 1/2 [=] 41 1/2

Drosera Recovery

Drops of 4 to 1000 placed on 7 leaves say (2d Plate) on Sept 2d 4th. 2˚ 45' P.m.

Sept 4th 8˚ 8˚ 15' a.m say 3 as only 3 red leaves examined & most of glands reddish,

[insertion:] 2˚ 30'

ie not very black. I had washed leaves on the 3d; but seeing colour added more of the solution. I now after 41˚ 1/2˚ — left added another drop & left for further 24˚ cut off piece from one of darkest leaves & carefully examined several 3 of short central tentacles: in those with much aggregated matter no chlor: grains could anywhere be seen, & the aggregated masses were quickly changing shapes.

In the disc-cells the grain swollen which seem first stage to aggregation. In 2 longer red tentacles in which there was hardly any aggregation in basal part I could distinctly see the chloro. grain on all the walls, & this makes me sure that there were now none in the short central tentacles, but & as these seem to be normally always present, I can hardly doubt that they aggregate Q.E.D.— N.B. Perhaps in plants which

[4v]

largely live on insects the chlorophy-protoplasm adapted to cease working from decomposing Carbonic acid, & all in cells works at the peptones

Sept 5th 8˚ 45' a.m — syringed washed sponged these leaves clean after 66˚

Sept 5 examined a red leaf of above lot & in short central t. no chl. grains, & much aggregated matter but in rather longer red t. in which little aggregation grains could be seen.

Some of the aggregated masses becoming granular, I suppose injured — I also examined a very green leaf & have seen in 4 of the shortest tentacles no good aggregation & plenty of chloro-grains — In a 5th short one spheres in upper part & here grains could be seen, but whether in same cells I doubt — some granular matter — Avoid very green leaves.—

Sept 7th 11˚ a.m Small fine red leaf one of the above lot — 2 very short central ts. with no aggregation at near base; I can hardly doubt that there had been aggregation, & now with plenty chloro grains in their basal greenish cells, — In a third short t. some considerable aggregation near base & here I am doubtful about any some irregular grains, though higher up same t. no aggregation & plenty of regular grains. aggregated masses becoming transparent & breaking up it small spheres.

(5

1881 Recovery Drosera Aggregation

Sept 5th. 10˚ 25'. put drops of strong filtered sol: of raw meat on 7 leaves, all reddish— marked by Pins with red Heads. Fresh drops added at 4˚. 20' P.m

Sept 6th 8˚ 55' a.m 22˚ 1/2 hr carefully syringed— petioles resting on big pins. — cut off t. examined one leaf in long exterior tentacles aggregation has not reached base & chloro-grains quite distinct — in 5 short central t.s. aggregation to very base & no trace of a grain — In some the these the aggregated almost white, with only faintest trace of greenish yellow, & such masses were in constant movement—

Sept 11' ie after 5 days 9˚ a.m— of the 6 leaves left, 1 killed, 2 much injured with outer t killed, central ones alive. 1 considerably injured & 2 fresh & vigorous with glands secreting.—

Examined one of latter, glands bright red no aggregation in glands (1) of short central t w still with much aggregation & some fine granular matter & no Chlo. grains (2) with plenty of Ch. grains & a few aggregated spheres. (3 & 4) some aggregation & some Ch. grains (5) very little aggregation & plenty of Chlor. grains.

(over)

(6

Same plants continued.

Recovery

I also examined one of the much injured leaves, with outer t' killed.— The glands of short central ones opake & pedicels brownish, some aggregation, some brownish matter— in some of the ts. there are minute sphere or grain which are arranged along walls of cells, & are apparently Chl. grains, but whether they would ever recover I know not & become true green grains.

(7

Recovery

Drosera Aggregation

Sept 12th 8˚10' a.m. some not very strong drops on 6 7 leaves of infusion of raw meat — tin-foil—

Sept 13' 8˚ a.m. after 24˚ examined half of one of almost the most affected & reddish leaf: central t. not so short as usual: agg: down to base & no trace of Chlo: grains — In 1 longer t. a very few gr in some few cells near base, where no agg. — No agg. in disc.

Examined (8˚ 45') a 2d 1/2 leaf — 4 short central t. with fine agg. to very base & not a single Chloro. grain — This leaf was fine red & with ts. less inflected than those of any other leaf.—

(Washed all the leaves at 9˚ a.m 13th (so nearly 25 hr meat-juice)

(I gave alcohol to the first of above slides; after few minutes all agg. destroyed & only brownish granular matter left in all the ts. whereas the chlorophyll grains in disc only rendered colourless, & now seem to be swollen. — In one of the short ts. after being thus rendered transparent by alcohol, in 1 or 2 cells a few Chloro. grain could be seen. —

All the above remarks mainly apply to the Chloro: grains attached to walls of cells. F. has tried & finds that placing aggregated ts. in boiling water, only granular matter left.

(8

(Recovery)

Drosera

Sept 15th— state of 7 leaves on which infusion of neat placed on 12th & removed on 13th (1) (2) (3) — 3 fully expanded & vigorous, (1 (4) leaf of which 1/2 cut away almost fully expanded & vigorous—) (1 (5) 1/2 cut leaf, rather injured) (6) 1 much inflected & somewhat injured. (7) 1 apparently almost dead.)—

(Examined rather short central t' on half of largest dark red fully expand & vigorous leaf.

1 tentacle some aggregation near almost colourless base — chlorophyll grains, distinct, but seem larger, less regular, & less regularly arranged than normal

2 ts. agg. strong to base; no distinct grains, but in one cell some fab large gr indistinct spheres, looking granular in middle, & which I suppose was forming Chlo. grains,

3 t. only a vestige of agg. & an abundance of grains.

4t.— hardly any aggregation in most of the basal cells, & here plenty of ch. grains, but irregular in shapes & irregularly arranged on walls — In 2 cells close to base splendid aggregation & here no trace of a chlorophyll grain, & yet cells from contents being aggregated, some grains adhering in pairs remarkably transparent, so that gr. wd have been easily visible.

(9

Recovery

I added iodide of Pot. to this 4th t., & the purple spheres lumps in the cells — which consisted of moving & living protoplasm, instantly broke up into pale brownish granular matter (very important for Geranium spheres). In the cells with pale purple fluid — only slightly aggregated, numerous bright spheres were formed ie, there was aggregation, but in before long the whole of these purplish masses were converted into brownish-purple granular matter with still some minute spheres,— The tentacles in about 3/4 hr. became very opake.

It was remarkable that many of the irregular Chlorophyll grain in the cells, which, as I believe had only just been formed, ran together & became confluent along the walls.

Sept. 16th 8˚ am cut transversally 1/2 leaf of one of the 2 greenest— in 4 short central ts. with some, (& a good deal in one t) aggregation, & in all grains, in those with only a trace of agg, an abundance of grains.

It is clear that grains are reformed early before all aggregation has disappeared — it is the first change. —

[9v]

After 4 days another leaf — in all 4 t. grains

aft 6 days 7 to examined — in all grains, & little or no agg, excepting in 1 t. — in which much agg & no grains. —

Aft 8 days 4 5 t. grains in all— some little agg. still

after 11 days leaf from above 1/2 cut & somewhat injured by the infusion & in which 4 t several ts were examined & did not exhibit a [illeg] . — Now 20 t' expand all of which seemed perfectly healthy

(10

1881. Recovery

Geranium Drosera

Sept. 18th examined another leaf, of above lot on which weak infusion of meat has been placed.

3 short ts with hardly any aggregation & plenty of Ch. grains

1 some agg — grains of Ch.

2 considerable agg. near base & yet some few grains

1 much agg. near base & I could see no grains, but the basal part rather opake

This leaf was rather fine & moderately red

Sept 20th —another leaf of same lot examined. —fine moderately red leaf. (1) short central ts, some agg. several grains of Chloro: near base. (2) t 1 like (2).— (3) very little agg, abundance of Chloro. grains (4) some agg, a good many grains.— I tried effects of squashing these 4 tentacles; but the chloro; grain wer not thus rendered clear or indeed at all visible. —

Sept. 23d, examined leaf of which 1/2 was cut off (p. 7) & washed on the 13th (infusion put on on 12th) & in whole leaf, 4 (fine red) 4 central tentacles were examined & did not exhibit a single chlor. grain. Now after 10 days, 10 ts were examined from the other half, which seemed perfectly Ø

[10v]

healthy & all the glands were secreting. Of these 10 tentacles, plenty of chlorophy grains could be distinctly seen in 8 of them, generally at base & beneath gland; but in one of these there was still much agg. at near base & here no grains, or doubtfully & irregular grains. In remaining 2 tentacles there was still much aggregation, & no regular grain, but apparently some irregular ones along the walls.

In the other t. leaf of which 1.2 was cut off & examined on the 13th, —which was not nearly so red & equally inflected was much injured by the infusion; here there is 1 of central tentacles much aggregation & no trace of grain; but now (23d) only 2 of central t' have recovered & in both of these innumerable Ch. grains from gland to base, unusually conspicuous

(From facts now given no doubt that the Chlor. grains are reformed.—)

(11

1881

Drosera Doubtfully worth giving

Sept 19th

Irrigated 3 short central t' on greenish leaf with strong sol: of C. of ammonia 7 to 1000 at 8˚ 20' a m (too strong

8˚ 30 granular matter & pale agg. masses forming every where

8. 35 granular matter to base — Chloro. grains every where distinct

9˚ some grain at base quite indistinct, I think have become granular —9˚ 5' basal cells now filled with pulpy greenish matter—I believe Chloro grain have diminished in size

10˚- 15' so opake I can see nothing. So it was next morning. I pressed covered cover glass, & flattened ts. & could no where see any chloro. grains; but do not feel sure, though I could see spiral vessel distinctly & think that I shd have seen chloro: grain— The aggregation had not gone far enough for endless small spheres to aggregate into a few large ones.

(12

1881 ✓ finished Drosera

Sept. 23d. — A slice of Drosera leaf made by F. examined. 2 short central t. with plain Ch. grain along whole length to base — not on transverse cell-walls. (Also near gland in long marginal tentacle— Added weak infusion of raw meat at 8˚ 45' a m. —

(A whole 2d leaf was likewise placed on slide in drop of same infusion at 9˚ a.m, & near oval gland in middle, between 2 elongated glands (both of which had undergone much aggregation) there were many plain grains

Sept 24th 8˚-a. m. no true aggregation only muddy structures: it certainly appears that nothing off slip present aggregation with infusion of meat. — very many chlorophyll grains every where plain —

In second whole leaf of which one long tentacle was placed under cover with the infusion, this morning at 8˚ 30' great aggregation could see nothing close to gland, but not far off by pressing the spiral vessel could see no grains; but a little lower down tentacle a few could be seen. (see back)

[12v]

These tentacles with splendid aggregation & as I thought to with no Ch grain in some part, were irrigated with acetic acid & when thus rendered clean & colourless in all 3 thus treated an abundance of grains cd. be seen!!! I suppose whole case an illusion. The instantaneous effect of acetic acid with very curious, the larger pale aggregated masses instantly become filled with small spheres & soon the outlines (as if a membrane) was alone left & then this disappeared & afterwards the small enclosed spheres.—

Action exactly the same as with Geranium

(I tried a t. which had been for 25 hr 20' in C. of am (4 to 1000) & the black ball soon became purple, but did not soon disappear; nor had they disappeared next morning, though rather paler & not so transparent.

(12 bis

1881

Drosera

Sept 25) A leaf which had been in C. of am (4 to 1000) from 10˚ a.m 23rd to 5˚ P.m on 24th ie 31˚ was left in Acetic A till 8˚ a.m on 25 & then examined. It is certain that in cells not far from gland with pale purple perfect spheres there were plenty of Chlor. grains, & I saw grain in many places

Therefore perfect aggregation here not necessary within all Chlo. grains from walls

Another leaf which had been left in diffusion of meat for some time as in last case, after the A. acid presented a muddy granular appearance, but I could see in many places Chloro. grains. We thus see that the aggregated masses from C. of amm & from meat differ in nature.—

Another leaf had been left in S Ether & was then examined in alcohol but I cd. make out nothing too opake glands black. —

(13

1881

Drosera

Sept 24th 9˚ a.m placed longest round-headed opposite petiole to in C. of Am. (7' to 1000) under cover glass— focussed to spiral plenty of Ch. grains visible near gland; visible even with next object glass (Sept. 25th. 10˚ 15' a.m. aggregation dark purple spheres; plenty of Chloro. grain close to glands) (26th 8˚ 30 still plenty of grains in parts where spheres— many of the grains are now spherical & appear detached. Can the process of aggregation have been stopped by too strong solution, or by pressure of covering glass? agg in disc & purple balls in epidermis cells. At base of one long t. there were spheres, purple in the centre (z)

Sept 24th 9˚ 15' a.m. placed second largest round-headed t. opposite petiole, in sol. of 4 to 1000— many ch. grain visible near gland by focusing the spiral & even before spiral; but not so plain as in above specimen.—

Sept. 25' 10˚ 30' a.m. agg. oval purple masses; a few chloro. grains can be seen near glands, (Sept 26 8˚ 30' I am doubtful about presence of grain so left for 1/2 hr in acetic A.— saw some grains — some of grains confluent — many very minute in parts.

(no agg in disc, so aggregation not carried to an extreme.

(z)

& surrounded by green. This interesting, as analogous to what I observed of dark purple surrounded by pale purple or white. When acetic acid added to green surrounding part & the wholly green spheres all disappeared.

There were infinitely many Ch. grains still left in the bases of these ts. In some there were grains in the epidermis of ts. & after the acetic acid, infinitely minute points were left adhering to walls where grains had adhered; but I did not succeed in staining them with iodide. These I suspect are starch grain try them. Some purplish-green masses were changing forms & were alike

Some grain distinctly confluent: I believe that grains slowly swell & are sometimes separated from walls & that the protoplasm round the starch active granules are withdrawn & become confl aggregated into spheres; but that this requires extreme aggregation & favourable circumstances

(14

1881 Sept 25

Drosera

A leaf was put in sol. of C. of am. (4 to 1000) on 23d at 10˚ a.m. 48˚ sliced today Sept 25' 10˚ 30' - to 11˚ no grain whatever at base of some tentacles beautifully clear from great aggregation — In disc endless green spheres. (are they green when dissected out) & where these abounded, the true chlorophyll grains were rare or quite absent. — Can it be that long-continued & great aggregation is necessary to act on the grain? —

 Are the spheres green when dissected out??

This specimen was irrigated with alcohol for rather short time & placed in glycerine & examined 26th. It is certainly true about base of ts splendidly clear. In many epidermis cells purple spheres discuss causes p 15 & no grains, & this occur when there is only 1 or 2 or 3 spheres; in some other cells well defined outer-epidermis cell p 15 several grains of Chlor.

In section oblique through pallisade cells; a multitude of smaller grains, & on lower side, a gradation can be traced from perfect grains, (where section very thin) to smaller irregular grains, to small sphere & to larger spheres, where these latter occur no or only few grains [insertion:] Conclusion

Sept 28' Rexamined same slide. I traced from elliptical Ch. grain to small swollen spheres, which attract matter round them, as may be seen Ø

[14v]

from opake centres & become larger; & then the small spheres often run together (of which I have seen many gradations) & continue to increase by attracting more matter, so that different tints externally— The exterior zone of different tint, which depends on where granules or chlorophyll grains are attracted from the walls of cells. In most epidermis cells no gr. but in some perfect & ordinary, but now colourless grains

Conclusion

(15

1881

Drosera

Sept 27 I examined several green leaves which had been for 1 or 2 days C. of amm of various strength & found no green spheres.— In epidermis cells in this & many other specimens dark purple balls; whereas surface of leaves are green & colourless; but there is the potentiality of turning red in these epidermis cells on disc, as in very old leaves the surface became red..—

Frank has examined 3 leaves, dark-red— intermediate & p very pale, & in upper leaves epidermis cells plenty of chlorophyll-cell, & we know that they somehow spread to epidermis of exterior tentacles—variable & this rather odd

In a specimen kept in sol. of C. of Am 4 to 1000, from 23d 10' a.m. to 11˚ a.m 27th, after being cleaned by alcohol showed in epidermis cells many spheres without any or many (no) chlorophyll grain — Now preserved in Glycerine.— Rexamined on 28th— Many of the spheres in the epidermis cells, showed a fairly well defined dark centre— R [illeg] in some of the →

[F62v]

purplish spheres, which then are generally elongated.

In many parts of slide, especially in middle of slice innumerable Ch. grains & at base of long exterior tentacles — Spheres of different colours in many parts of the slices — generally several small spheres in guard-cells of stomata — In many epidermis cells certainly dark spheres without a single Chl. grain

(16

1881

Drosera

Sept 27th leaf reddish placed in Sol. 7 to 1000 at 4˚ 30 Pm on the 26th & examined at 11˚ on 27th — in epidermis cells many dark spheres with only here & there chlorop. grain, which I think are becoming confluent with sphere.

In base of 1 long to many green balls (F saw) not due to underling Chloro. grain, & these balls were clearly in process of aggregating it larger spheres.— In these cells no grain cd be seen, though visible visible in other cells with purple balls— Now placed in alcohol in saucer. for night, & then glycerine added (see p. 20)

Sept. 28' Reexamined some slides — in most epidermis cells upper & lower — one, but not rarely 2 or 3 almost black spheres, either separate or touching & sometimes partially confluent — in none of these cells any Ch. grain; but in a few parts granular matter, apparently disintegrated Chl. grains. In rudimentary t. & guard-cells spheres — In central cells, between upper & lower surfaces, many dark spheres & in such cells, no Ch. grains, but in other parts Ø

[16v]

a multitude of Ch. grains & this shows that the manipulation manipulation has not injured the Ch. grains.

In base of some of short ts certainly no Chlorophyll grains.— It Three good slices carefully examined — It is certain that Ch. grains become aggregated around other contents of cells

(17

1881

Drosera

Sept. 29th Leaf full-grown fine red, lately from common in sol 7 to 1000 at 12˚ 20' 27th, examined & sliced 3 leaves — very numerous elliptical & spherical Ch. grain, which become blue with iodide— also innumerable minute spheres, far smaller than the ordinary Ch. grains) which became orange with the iodide. In very many cells compact spherical aggregations of orange-brown matter, but no translucent ordinary aggregated spheres, except within tentacles— Case very odd is too late in season? has all protoplasm been withdrawn or used in forming the immense quantity of starch?— I never saw such a case before. I suppose Or was aggregation stopped by some unknown cause? could the solution have become too weak; I think not so, for leaves excessively blue & flaccid — they were probably killed before aggregation complete. It certainly appeared that the fine granulo-spherical matter replaced the Ch. grains.

No green translucent spheres in epidermis cells, only the brownish-orange spherical granular balls.—

[in margin:] Too long night??

(18

Drosera

(Young red leaf, left in sol. of 7 to 1000 from 7˚ 30' a.m on on 4th Oct to 9˚ a.m on 5th — hardly any spherical aggregation in disc with innumerable Chloro: grains & a few green spheres (a) Back in one part. Want of agg. here I suppose the leaf too young. — In one slice palissade & other cells filled with green pulpy in which matter, slightly aggregated, ie. slightly contracted from walls of cells, I therefore cleaned with alcohol, which rendered masses pale brownish, but not a Ch. grain cd be seen. The presence of such green matter due to breaking up & confluence of Ch. grain important

(Young green leaves in C. of Am 4 to 1000 for 48 hr.

In some section the Ch. gr. have coalesced into bars—plenty of green balls in epidermis & elsewhere— Wherever the green spheres are well developed few or no grains.

One slice of another young green leaf very instructive,

(a)

There were in epidermis cells of this leaf dark purple balls, but still some ch. grains; these not having as yet aggregated. —

(19

Drosera

here there was in one slice groups of innumerable shiny greenish spheres, far smaller than Ch grains, graduating into greenish spheres larger than Ch. grain; & where these occur no ordinary Ch. grains. I believe that Ch. grains swell & then dissolve into pulpy greenish matter which with cell-sap aggregate first into very minute spheres, & then into larger ones. The purple cell-sap in filaments & the sap in epidermis which turns purple however, often aggregates before the Ch. grain. (So in Spirogyra the cell-sap first becomes granular)

(20)

Drosera

Oct 7th

Looked again at best slice in green young leaf (1) in Glycerine; all that I said true — endless spheres in epidermis & other cells — some still greenish— not one Ch. grain. It could be seen in some cases that large spheres due to union of small ones ― Some of the smallest spheres are not sphere but irregularly shaped. — Larger spheres solid for some are cracked — Whether Ch. grains always break up into small spheres seems doubtful — many swell & soften & blend together without this intermediate process. — I believe from cases in which gr. can be seen forming bars that they probably do aggregate without the intermediate condition— Some spheres with defined darker centre; this definition shows interval of time & so white zone in round speres in tentacles.

In another green slice be many good green spheres, with still many ch. grain & apparently naked particles of starch.

(21

Dec. 22d 81.

Drosera. rotundifl.

Young, small, bright green forced leaves—left for 24˚ in 2 drops of 1 per cent. sol. of Osmic A. to 1/2 oz of water. (438 gr)—

No effect on Ch. grains at base of longer to or in disc— In epidermis cells of ts innumerable blackish spheres, very minute.—

(Roots too black to make out anything — reddish globules within root-hairs.)

Same kind of leaf left under cover-glass for 48˚ in same sol. Age & state or pressure has checked aggregation— but in some of t. only granular matter — in other grains still visible— many cases of a few grains still on walls, confluent — in other cases all grains, still green accumulated at bases of cells, forming, however, a very small masses — granular matter in all epidermis cells —

After 24˚ plenty of gr. distinct at base of longer tentacles where hardly any or none can now be seen. (over)

[21v]

A whole similar plant left in do sol, for 48˚ & leaves flaccid— (sol. too strong?) did not aggregate well — granular matter with ch. grains in places heaped at bases of cells. —.

Dec. 26. Another small green leaf of same kind left for 47˚ in sol of only 2 to 1000 Ammonia? — no ordinary spherical aggregation, but in epidermis cells of ts numerous irregular, minute, more or less confluent granules.

Before the A. innumerable Ch. gr. in all the cells & now only a few, 2 often united, & irregularly distributed — In many cells the Ch gr form a greenish mass at one end of cell. —