(1

Nov 12th

Sarracenia purpurea

Slice of fresh leaf in water, some few almost colourless spheres in epidermis cells & no orange ones near spiral vessels or elsewhere — Slices were irrigated for 3 or 4 hr into C. of Am (7 to 1000) & I believe some spheres dimly appeared.

(Piece of pitch in C. of A. for 24˚ 4 to 1000.— many colourless or pale green sphere in epidermic cells & some in all parts of parenchyma, of which some were bright orange.— many such orange spheres & oddly-shaped agglomerated masses ne close to spiral vessels— In many parts Ch. grain distinct (some swollen?) in other parts much fine crushed granular green matter: — Agg in guard-cells or glands — In a 2d spec. treated in same way no doubt about the orange aggregated masses. The orange masses in parenchyma often contain granular matter in centres. The sphere & grain are confluent in all degrees— Some orange masses in the slices, were irrigated with C. of A. (7 to 1000) & all watched for 2˚. 15', but did not change form in any plain matter.

It was clear this irrigation had greatly increased the orange spheres & agg. masses, for they were now present in nearly all the epidermis, adjoining & middle parenchymatous cells. In all these cells, there was green granular matter.

(2

Sarracenia

(Piece of pitcher left in infusion of almost raw meat for 24˚, produced far less effect than the C. of Ammonia— Ch. grains very perfect — no orange masses anywhere — some few transparent spheres in epidermis oil cells, as in the fresh leaf examined—)

Nov. 13— Same pieces of Pitcher in 4 to 1000, (but with some of 7 to 1000 added yesterday evening, after 48˚, now innumerable orange spheres & aggregated spheres — some dew pale spheres — in epidermis & most other cells. — Cells generally filled with pale uniform green matter, which under certain light can be seen to consist or to contain ghosts of enormously swollen Ch. grains. Some appearances lead me to suspect that the orange spheres are swollen Ch. grains, aggregated & which have absorbed some orange matter; but safest conclusion is that orange sphere are aggregated cell-sap. (analogous to agg. matter in Euphorbia peplus)— Some not swollen Ch. grain, perhaps contracted.—

Orange spheres abound on side where spines. — I believe Ch. grains in epidermis on inner side — Acetic. A pales somewhat the orange masses, but does not break them up. It is very remarkable that after about 1˚all the [much] green matter in cells has been turned by the acetic A. bright yellow, of same tint as the spheres & aggregated masses— This shows some clear connection between the ch grain & the spheres.—

(3

Sarracenia pur

Novr 17th

Transverse Slices of fresh pitcher near base, but above where insects collected — Inner epidermis everywhere with Ch. grains. Exterior epidermis some grains — The adjoining parenchyma cells full of grain, with no exception — In middle parenchyma many grains , but some cut cells or intercellular spaces empty —

In exterior epidermic cells in parts purple fluid — Spines exteriorly. I saw what I thought were colourless sphere in epidermis, but I believe that it was rounded cell-walls below upper wal layer.— or dry part.─ Numerous stomata exteriorly; interiorly projecting brown mass, having nearly structure of stomata.. Acetic A makes ch. rather yellowish, but no great change in 1/2 hr. — No orange spheres near vessels or elsewhere, but I saw some colourless sphere where vessels cut rather longitudinally, & I think that these disappeared after acetic A. — Nov. 15th After the Acetic A. for all night single Ch. grain colourless, in masses look yellow— Most of the grains quite distinct cd be seen in in l inner epidermis — curious spines on this latter inner side.

(4

Nov 18'

Sarracenia

A piece of pitcher kept for about 49˚ in Sol. of 7 to 1000 C. of A., showed beyond doubt that the Ch grains had more or less become completely confluent, but they had nowhere formed spherical balls & nowhere except close to vascular tissue were there orange sphere & curiously shaped masses.— These latter were not affected by immersion for an 1˚ about in pure alcohol—

Infinitely many perfect & distinct Ch. grains still present. In some few of outer epidermis cells (after cleaned by immersion in alcohol, all the Ch. grain had become confluent, but yet did not form perfect spheres, & they were still green.— The layer of cells with Ch. grain & therefore green is thicker on outer than on inner wall of pitcher —

(Nov. 19th the same pitcher now kept for 3 days & 3˚ 1/2: now in several of the inner epidermis cells orange masses formed by complete confluence of several swollen & discoloured Ch. grains may be seen, with no true Ch. grains left.; but no true spheres— The aggregation is shown only by outline, consisting of several curved surfaces.— Orange spheres — near vessels — some balled or glandular Ch. grains in parenchyma cells ↘

[4v]

chiefly glandular matter in outer epidermic cells — There are innumerable true or ordinary Ch. grains still present.

(Nov 19' At bottom of a pitcher I found a mass of decayed insects, & made sections of adjoining walls, Ch. gr. are swollen — no aggregation no orange masses near vascular bundles.)

(5

Nov. 20

Sarracenia

Young pitcher with much purple in 7 to 1000 for 46˚ many crimson spheres in epidermis. I could not see whether all ch. grains absent except in one 3 cases in which seemed to be so.— In parenchyma some large green spheres, which I have no doubt were the modified balled grains, but some grains still in some cells— where orange spheres ought to have been i.e. close to vascular bundles, only masses of brownish granules, so I suppose the orange spheres had disintegrated.

In superficial slice on outside it seemed as many Ch. grain where there was purple fluid as elsewhere, but here no spheres — either broken down or never existed.

(Nov. 20—Fresh Part of old first pitcher observed, after 50˚ in sol. of 7 to 1000 more aggregated balls of orange colour — in epidermis & parenchyma — in some case apparently not one Ch. grain left, but generally some few left or granular green matter.— The presence of similar orange spheres near vascular bundles shows that orange spheres not primarily due to Chlorophyll, but this will not prevent the balled & confluent masses, certainly formed from Ch. grain, united with the matter which forms the orange spheres. — Saw good cases in which slice a little obliquely pressed in which there were in epidermic cells pale yellow with green & yellow perfect spheres, with no ch. grain in same cells

Spec. found

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Nov 21

Sarracenia

Pitcher placed in sol. of 4 to 1000 on 19' 8˚. am. & on 20' 8 A.m solution changed into 7 to 1000 ─ result splendid aggregation as in former instances. — In outer inner epidermic cells endless sphere, translucent (& many called masses) & in some of these cells no ch. grains. In some parts the spheres were of a pale dirty green, of exactly the same tint (seen through a weaker power) as the pale ch. grains. In many cells 2 spheres— Spheres occur in the parenchyma & in the outer epidermis. In the case of both the orange & pale spheres in epidermis, there were many cells with no ch. grains —

Iodide of Pot + Iodine irrigated one of slices. The pale spheres now become orange & the cuticle also orange. In many cases there were blue grains in some cells or coating the orange spheres & aggregated masses—

Much starch near vascular bundles— After Iodine it was still more clear that in many of green epidermic cells, (almost all contain when fresh Ch. grains) there were now no Ch. grains, as these had blended with the cell-sap to form↘

[6v]

the spheres or irregular aggregated masses.— such grains of Chlr: could have contained no starch, the bright orange spheres with bright blue grains of starch in some cell looked very pretty

[7]

Small young leaf— much granular or crushed green granular gr— many perfect — many swollen greatly — some small colourless spheres: highly [illeg] in many of cells looking like oil globules & dissolved in alcohol — Chlorophy in both epidermic cells — not [illeg] most, I think exteriorly

Old leaf — do — no orange spheres, but cells with chlorophy close to vascular bundle.

Jan 19th

Sarracenia.

Piece of youngish leaf not examined before & piece of previously examined old leaf, immersed in distilled water for 3 days — 4 brw — no yellow spheres nor vascular bundle or grt elsewhere — Many Ch. gr. either smal [illeg] or rendered granular— many perfect — many swollen — colourl in some of section several highly [illeg] colourless globules in cells of various kinds— apparently oil, quickly dissolved in absolute alcohol.— Ch. grain in cells close to vascular bundles— some small grains in both epidermis layer, but chiefly, I think in exterior epidermis.