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1881.

Spirogyra

Oct. 1. on adding under cover glass drops of sol. of 4 to 1000 contents of cells became granular which ultimately collate into masses & after 1/2 h. the green spiral band begins to contract into lumps on lateral walls, with transparent spheres, here & there scattered like nuclei — (Alcohol destroys green colour, (not the lower granular matter) leaving I think ghosts of the green masses, & scattered transparent minute spheres, the nuclei. —

(Spirogyra (cross a?)

Oct 4th placed some filaments under M. & irrigated at first with C. of A. 4 to 1000, afterwards with 7 to 1000 without first draining away water with blotting paper. — The Ch. Band soon appeared dotted, but this was owing to excessively minute brownish granules which appeared throughout in the clear fluid filling the cells. In short time 2 fine tails to the bands in 2 cells were slowly retracted & disappeared. The irregular projections on edges of bands were withdrawn, so that bands became more regular

11˚ 25 grains in clear fluid rapidly forming: bands more transparent & less green.

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Spirogyra

11˚ 30' Bands generally in contact with transverse position in all the cells

In 2 cells the green bands formed circles round nuclei — There is an appearance of hyaline spheres within the bands in several places see to this

12˚ 50' made sketch of Bands —

at 4˚ 5' P.m. In cell (3), which had been lined on 3 sides with Band, the lining on lateral wall became very thin near one end of cell, & with in by 4˚ 15' ie few minutes had disappeared; with the upper mass contracted into a single pyriform mass— not figured— The Band at base formed a dumb-bell shaped mass, which afterwards became cylindrical

In cell (4) by 4˚ P.m. there was a pyriform mass & small green spheres at opposite ends instead of mass as figured at 12˚ 50'; I saw plainly this pyriform mass change into an oval, which subsequently became somewhat pyriform.

(3

Oct 4th

Spirogyra

Irrigated about 11˚ 10' a.m) Spec pr irrigated as said above The same filament at 7˚ 10' P.m. cell 3 had 2 green ill-defined balls at upper end; & 2 irregular balls at lower end, connected by obscure green band. In Cell (4) the upper pyriform mass had become oval with circular hollow close to inner side where the granular matter could be seen. At 9˚ P.m no change of form.— In another filament one cell still had spiral band.

[in margin:] The green matter very pale.

(Oct 5' 8˚ a.m kept in the sol. all night—

In cell I. 5 green balls with ill-defined margins

In cell 2. 4 do balls, but one had 2 small satellite

In cell 3. 2 balls at base, both connected by very thin fibres with larger oblique mass in upper part of cell. This cell originally (ie at 12˚50') had no trace of a band down whole of lat side.— What curious successive changes!

In cell 4. one round ball at basal left hand corner, & & one elongated mass, down opposite side, with the circular hollow more elongated.

Acetic acid, irrigated, does not act on granular matter or green bands; but makes cells clearer. — A good deal of Chlorophyll left.

(4

Spirogyra

Oct. 5. A tuft was placed 11˚ 40' a.m Oct 4th 21˚ in a sol. of only 2' to 1000 & examined on 5' at 8˚ 30'

Some filaments with perfect spiral band. In others with 1 or 2 bright green spheres, & perfect gradation between ba spiral band & sphere were seen in one case, as the bend was contracted into a short corkscrew, with only a trace of a spire left. Some brown granular matter in all the cells, & generally the granular matter aggregated into balls, distinct from the green balls ― This important for Drosera.—

The balls of granular matter appear not to be solid but to encase a sphere of fluid.—

In other cases the granular matter seems to encase walls of cells, especially the lower wall & sometimes seem mixed with green matter.

A weak solution acts better than a strong one.

Is granular matter ever entirely mixed with green?

(5

Spirogyra

Oct 6th 11˚ 15— relooked at spec. which had been in the sol (2 to 1000) since 11˚ 40 on the 4th still ghosts of green band, as little spots, in most of cells — in some cells a spiral band, — in others spheres or oval lumps— much granular matter, somewhat aggregated & attached to walls of cells

Alcohol in about 1/2 hr removed green colour, did not act on glandular matter, though it cause a lining membrane with the granular matter to shrink from cell walls.

(Spec. put into sol. of 1 to 1000. Oct 6' 8˚. a.m, examined Oct 7th 10˚ 15', 26˚ exhibited plenty of spheres & oval; often united by finest green thread in one case 3 spheres thus united, & in position of spiral band. Where thread arises, mass— pear-shaped, & I saw one separate & the plane became almost spherical. There was one 1/2 moon-shaped bright green mass — Not very much granular matter

(6

Spirogyra

in these spec. in weak solution.— Granular matter altered to membrane which often lies within the green masses —corresponds to the kind of bag found in Drosera.— In naturally dead spec. green matter & granular both enclosed in membrane shrunken from walls which is exterior to green masses,— In naturally dead specimens. In some specimen granular matter in a spire sometimes in one or 2 spheres.

Hyaline rounded points within the green matter; starch?

S. Ether caused no particular effect in 1/2 hour on green matter or granular matter.

At 11˚ some filaments irrigated with sol of 7 to 1000 & instantly granules appeared in 3 young cells, which were as transparent as glass. By 1˚ 45' some green spheres were formed & one of spiral bands contracted.

At 1˚ 50' added acetic A. & then iodide (which I believe removed all the granular matter?!!!) Some of the green masses become a little blue in centres but not so the hyaline spheres.—

(7

Spirogyra

The spec. which were placed in sol. of 1 to 1000 on Oct 6th 8˚ am, on Oct 12th 10˚ 30' a.m. look quite green & fresh, whereas the spec kept in basin are all dying or dead, & so are those in the pond near Holwood.— The green matter consists of spheres or ovals or of normal spiral bands — It is clear that weak solution of C. of amm not injurious—

Still much brown granular matter, as much pulpy as ever.— (It is not acted on by Iodide of Potash in from 1/2 to 3/4 of hr.

Oct 14th examined fresh spec. of 3 or 4 other spire of conferva in sol of C. of ammonia of different streak — In one kind in which whole cell-walls dotted with Ch. grains— There was some aggregation & there the Ch. all became finely granular & afterwards no change—

In one sp. with very thin filament — apparently no change — In another much aggregation into spheres — The action of C. Am might aid in distinguishing species. ↘

[7v]

On spheres first described (Oct 14th) N. & Phosphate of ammonia 4 gr to 1000 & nitrate of amm 2 gr to 1000 did not produce so much effect, [illeg] cells with granular greenish matters & other cells with the chloro: grains very little affected. C of Soda 2 gr to oz (or 875?) produced no effect