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Euphorbia myrsinites

Nov 5th.

Roots of a young plant with only 4 leaves besides Cotyledons immersed in sol of 4 & other roots in 7 to 1000 of C. ammonia. There were lateral rootlets, no agg. in exterior cells, or brown masses above root-cap. — but I saw agg. matter above round ؟ spiral vessels. Lat. Roots

A seedling with only 2 true leaves, after immersion for 2° 45 in sol. of 4 to 1000, no exterior agg. in the primary roots or in 2 lateral rootlets. The end of the primary root was now frayed with needles & examined & now I saw with absolute clearness several tubes with brownish agg contents closely surrounding the spiral vessels. One of these tubes ended in a blunt point. In one I saw what appeared like a transverse partition; & in another which must

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Euphorbia myrsinites

have been, for there were no transverse partition of neighbouring cells to be see through. Transverse [cone] cell point the proximal one was gorged with aggregated matter & other with only a few fine grains, & the separation was most distinct, by a slightly rounded partition [sketch]

The tubes or very long cells were narrower than adjoining ordinary cells & walls walls very thin.

Nov. 6th The lateral rootlets mentioned in last page left in the ammonia for 23° & reexamined this morning — saw long tubes full of granular matter in middle of rootlets & I think some transverse partition.

(A radicle of germinating seed for 23° in sol. of 4. to 1000 long tubes full of granular matter, but radicles very opake— Innumerable oil granules everywhere, which ran together when irrigated with S. Ether — Can it be grain in tubes?

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Euphorbia myrsinites

Nov. 8' — A root of seedlings cut cut off from seed was placed in caustic potash on the 7th at 11° & examined today (8th) at 9° — It was cut longitudinally & showed several tubes (no transverse partition) near spiral vessels full of agg. brownish granules.

I cd see none in exterior cells.— Does C. Potash cause the agg, or is it already in the radicles & not dissolved by the Potash —

Radicles very clear — specimen now preserved in glycerine & water.

(I have now looked at fresh root, split longitudinally & left for about 3° in glycerine & water, & there are many endoderm tubes full of aggregated matter, & endless amount of more or less hyaline aggregated granules in all the parenchymatous cells, outside the spiral vessels & endoderm tubes.

(I also saw granular matter in tubes after acetic a. which does not make roots clearer.

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Euphorbia myrsinites

Nov 7th — Plant bearing about 16 leaves in C. of A- 7 to 1000 from 8° 10'. 11° 40' — Lateral rootlets no agg in the exterior cells, but endoderm tubes with agg. matter.— (a) (Back)

Radicles of germinating seed of do with endless hyaline spheres in all parts, appearing like oval, & shown by pressure to be fluid, often containing granules,─ These granules are not dissolved. by S. Ether for about 30'.

I sa In specimen of radicle which had been subjected to C. of ammonia, acetic & twice to S. Ether, I saw at one end (which)?) several short elongated tubes, elongated pointed at both ends, containing granular matter — Do these generate the exterior cells with granular matter? Lines of starch, in not grain visible running up the radicle. — The hyaline grain not coloured by the iodide. I have been looking again, what I have considered as separate tubes may be only symmetrical contraction of the aggregated contents in tubes; but in one case

[4v]

Rexamined thin roots of this plant after 48° in C. of A. 4 to 1000— No alternation of granular cells near surface— but numerous tubes with granular matter in centre.— When granular matter white hyaline — generally no division of tubes into cells—

When brown & old— many most distinct divisions so as to form elongated cells.— many elongated & apparently almost free undivided cells, not near tip or base of root — apparently growing at both ends & full of granular matter. — Be it remarked this is not a radicle of a seedling, but lateral branch of moderately old plant.—

Nov. 16th rexamined this specimen.— preserved in glycerine.─ There are some brown cells scattered near surface, but not forming regular alternate rows, except in one place. & there are cells with brown granular matter in endoderm & of course the milk-tubes. — I added cold Caustic Potash at 10° 30' A.m. 15' & now 8° 30' 16' all the brown granular matter in cells has disappeared & contents of milk-tubes in this one specimen have disappeared except in a thick part of root.

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Euphorbia myrsinites

a tube seemed to turn out of course & point to penetrate to one side. — I can now see browner aggregated matter in many true cells, but not so near the outside, nor so continuous, as in E. peplus, — I think this the sole distinction.

Nov. 9th— A fresh seedling (nothing done to) sliced longitudinally & examined in glycerine — many tubes near spiral vessel & some but little within epidermis, full of hyaline, almost colourless, coarse irregularly shaped, more or less rounded grains.— The tubes are thus rendered somewhat stiff, so this at torn ends, they projected.—

At 11°. washed & irrigated with C. of A. (7 to 1000) — At 2°. P.m only change was that in many of the tubes the granules had run together & formed globules looking like oil, but I have no doubt— from former experiments not oil. — Nov 10' reexamined with same result — There seem certainly to be tubes, not cells, with granular matter in same relative position where the cells are in E. peplus.—

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Euphorbia myrsinites

Nov 11th

Root Radicle of seedling after 24° in sol. of C. of A. (7 to 1000) & then long kept for several days in glycerine & water, it is certain that there many long milk-tubes with agg. matter round the spiral vessels & near the exterior. A radicle kept for many days in bottle of S. Ether still exhibited many of the highly refractive globules like oil-globules.

(Nov. 14th radicle of seedlings after 20° in C. of A. 4 to 1000 was heated several days ago in C. Potash & left ever since in it — torn open the many milk tube running down to extreme tip, with hyaline agg. contents a beautiful object— Contents clearly not dissolved by caustic Potash — Iodide of P. was added this stained agg contents — yellowish.— some starch at extreme tip of root-cap.) (After long immersion in acetic acid not preceded by C. of amm, I could not see contents of milk-tubes nearly so plainly.) But cut longitudinally & placed in glycerine & water on the 8th, examined today (never in ammonia) & in some of milk tubes grains as well as aggregated as in the ammonia specimen??