[46]

Cœlebogyne ilicifolia (Euphorb?) — Roots immersed

Fresh roots with no blue cells — innumerable oil-globules no starch — some hyaline globules — After immersion for 21° in sol. of 4 to 1000 — many separate cells in parenchyma & close to vascular bundles of the thinnest & moderately thick rootlets,  coloured blue, more or less dark, & sometimes greenish. — In the blue cells granular matter & sometimes oil globules— S. Ether dissolved the oil, but did not affect the colour of the blue cells.— I shd think the colouring blue matter was in cell-sap. — I am doubtful whether the A. has caused any agg: of granular matter in the epidermic cells, but I believe apparently many more hyaline colourless globules in the cells immediately beneath epidermis than in the fresh roots. (Dec. 27' The fresh roots & slices above described were irrigated with 7 to 1000 for 20° & now there are in both plenty of the blue cells in all the specimen & I think more brown granular matter. The blue tint was ↘

[46v]

not caused by about 1° of irrigation. I am almost certain that the very minute grains in the blue cells are themselves blue. —