(5

Aggregation — Euphorbia roots

with brown walls & little irregular masses of granular matter in centre.— We see then that there is difference in contents in corresponding rows of cells.

I have now compressed roots in alcohol & at exact spot where the chains of squares occur, the cells are filled with brown matter, but no doubt the roots with C. of ammonia present a very different appearance.)

(Aug 12 Sp Root exposed for 2° 35' to old sol. of Phosphate of ammonia (1 gr. to 1 oz) no effect.—

(13th) same roots after 24° absorption — rather opake, brown aggregation near tip not nearly so distinct as after C. of ammonia; — but higher up with generally alternate rows of granular matter coarser grains than those after C. of ammonia & sometimes not so regular, more like the effected of Alcohol, yet grain more spherical & regular, I cd see no movement.— In the rows with no granular matter, the primordial utricle drawn from the walls to greater or less distance.— No movement in these contracted utricles.

(6

Aug. 13th

Euphorbia roots left in sol. of Fuchsine 1 to 50, 100,000 for 18 hr. no aggregation, but after drops of C. of amm. (4 to 1000) added to cover glass well marked aggregation.— Here & there a cell was stained by the Fuchsine much darker red. —

A l Immersion for same time in sol of 1 to 10 50,000 of Fuchsine had caused aggregation in the alternate cells; so I suppose is poisonous.—

A roots stained by sol. of Fuchsine of 1 to 50,000, was left for 21 hr— in strong sol of C. ammonia 4 to 1000 & the aggregated matter now has a disintegrated appearance— the squarish lumps being transparent in middle.—

(7

Aggregation

1881

Aug 13th exposed Euphorbia peplus large tuft of roots for 36' to C. of ammonia (2 to 1000) & then washed & placed in water— The tips of 3 roots which were examined, exhibited much granular matter. On 15th after 47° 30' examined 3 tips, from under water in one much granular matter just above cap, but not compact & dark brown & much fine granular matter in cells higher up root. But in 2 other fine l thin & short lateral roots, only a trace here & there of granular matter & I think this matter is dissolving. Examined 3 more roots & these with plenty of granular matter:

Aug 17th 8° 30' a.m examined tips of 5 roots, which had been kept all through 4 days under damp peat & in 4 of these roots as much (or more) granular matter as at first! In one very thin short lateral roots only a trace of granular matter; this latter alone looks as of granular matter dissolving— one might conclude that roots had been killed, but then plant looks healthy; can it absorb water through dead roots?? 15 Aug. 28th looked at several tips of these same roots, kept (over)

[7v]

under damp peat, & aggregation as plain as ever after all these days, but extreme tips of all these roots appear rotten & dropped off, so I believe C. of ammonia simply kills the roots. —

(7A

Aggregation. Euphorbia

Aug. 19th Euphorbia myrsinites — seedlings seeds with root just developed about 1 inch long placed in sol. of C. of Ammonia for 2 hr & then for 23 hr no plain aggregation. — Other roots left for 21 h' in sol. of 4 to 1000 showed conspicuous aggregation but this confined not to cells, but to many vessels occupying same situation as the external cells of Eu. peplus. —

Aug. 20' roots of Eup. peplus left for 22 h' in sol. of C. of Am of only 1 to 4000 water, & in some of these distinct aggregation; in others slight & in some I could not distinguish clearly any aggregation— planted in Peat Aug 20' 8° 30' a.m.— (a) (Back)

Aug. 21. Eup. myrsinites tips of 3 radicles touched with caustic, but m much too much touched, so that whole tip

[7Av]

Aug 28th morning — 8 days examined several roots — seem brownish & flaccid— in 2 or 3 the tubes distinctly contained aggregated matter. — No redissolution evident.—

If I try again keep roots in water.

(7B

Aggregation— Euph. myrsinites

killed & rotten, — at least (examined after 15° 30') in 2 of these specimen distinct aggregation in the milk vessels— One fresh root out of damp air, like the others & of same age no aggregation, though vessel could be plainly seen. — One root has a kink & on both sides for short distance, there was aggregation, but it disappeared further off.—

(Nov 5. 2° P.m looked at some 3 rootlets which had been C. of ammonia since Oct 30' — in 1 no granular matter — in 2d good granular matter — in 3 little brown translucent spheres in wall if each epidermic cell— added Caustic Potash.—

7C

Euphorbia peplus. Aggregation.

Roots placed on Aug. 17' 9° a.m in 1 of Carb. of ammonia to 2000 water & left till 2° 15' P.m, when 4 tips were examined, & dark brown aggregated matter was seen near under root-caps & higher up the generally alternate vessels with granular matter.

Roots washed & laid under plate Re examined on Aug. 24th 7 day 11° 30' a.m. & roots appeared flaccid & brownish as if half or wholly killed. The brown aggregated matter under root-caps more transparent, & the spherical matter higher up roots now with few spheres, but only brown granular matter