RECORD: Darwin, C. R. [1881].11.25. Sarracenia - Roots. CUL-DAR62.87-89. Edited by John van Wyhe (Darwin Online, http://darwin-online.org.uk/)
REVISION HISTORY: Transcribed by Christine Chua and edited by John van Wyhe 5.2023. RN1
NOTE: See record in the Darwin Online manuscript catalogue, enter its Identifier here. Reproduced with permission of the Syndics of Cambridge University Library and William Huxley Darwin. The volume CUL-DAR62 contains for Darwin's papers 'The action of carbonate of ammonia on the roots of certain plants', 1882, F1800 and 'The action of carbonate of ammonia on chlorophyll-bodies', 1882, F1801.
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Sarracenia Roots
Some Three Two white rootlets placed in C. of A (7 to 1000) 1°. 10' all the rows) of exterior cells with brown granular matter, but much darker in some cells than in others. In some parts cells contained [illeg] the granules, large oval & occasionally sphere masses with some few spheres of transparent aggregated matter, like protoplasm, — & in the midst of some of the granules — Some, I could see no movement in these masses — The cells found at the outer similarly shaped spheres, but of a few [2 words illeg] Near vascular bundle cells with transparent pale brownish yellow somewhat contracted matter, generally in ovals, occasionally in spheres, — on these rootlets, I saw no root-hairs, except high up 2 or 3 short points p 8 After [illeg] 24
Nov 25. Examined 2 rootlets kept in water from yesterday & (p. 10) no aggregation— irrigated them with sol. of 7 to 1000 & in 20' clear, (but not so distinct as above) pale brown aggregated masses could be seen in the cells near the tip
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Sarracenia
Nov 25
There are more— root-hairs than I had supposed very irregularly distributed & generally more on the part of the root-lets which have remained pale-coloured, than where they have become very dark.— These root-hairs are not so transparent, as is common, common but sometimes contain some very fine granular matter & the shrunk protoplasmic lining yellowish.— As far as I cd see the Hair do not arise from empty cells— (Roots kept for 24° in sol if 7. to 1000 — tips of many quite black; in outer cells sometimes filled with orange granular matter, others with hyaline orange oval of spherical or dumb-bell masses— sometimes, near & inside vascular bundles many oval & spherical orange hyaline masses an aggregation of small partially confluent spheres of different tints of orange — Certainly much aggregation —
Also the roots kept in 4 to 1000 sol for 18°—
Some many of the aggregated hyaline masses are very oddly shaped, approaching to that of dumb-bells—
I made transverse section of the finer rootlets. & of
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Sarracenia
a moderately thick root — In thin rootlets, the 2 exterior rows of cells with orange spheres (it is clear that the originally brown masses turn orange) — In the large parenchyma cells many starch grains, which turned blue with iodine — round & in the most of the vascular bundle many oval & spherical orange masses.—
In epidermis of thicker roots the cells filled with granular orange matter, instead of the aggregated oval & spherical masses. —
Citation: John van Wyhe, ed. 2002-. The Complete Work of Charles Darwin Online. (http://darwin-online.org.uk/)
File last updated 23 July, 2023