(1

Nettles. Urtica

Roots put in Nov. 15th 4° 45' P.m— sol of about 6 to 1000, examined on 17th 2° 30' P.m — certainly some alternation of brown rows of cells & colourless ones.

Examine a root & then add C. of ammonia & see of the brown appears. — Tear open.—

(Nov. 18' Some roots kept in water for several days had rows of brown cells, (& in one cell granules) & I believe the brown matter aggregated into small elongated brown masses in some of the cells after 10' irrigation with C. of A. 7 to 1000— left all night. then irrigated.

Many apparently starch grains in roots — 19th 9° a.m 24th These roots now much browner & show clearly alternating rows of brown cells & colourless cells, within as I believe, the epidermis — The fine transparent rootlets show much less — no or very little aggregation. — Tore open — some granular matter— some dark brown agg. small masses. — (I do not feel sure that the certainly alternating stripes of brown are not due to the shrinking & browning of the primordial utricle.)

(Box — roots too thick & opake to be clearly visible.)

(2

Urtica

Nov. 23d

Some roots kept in water for 24° & then Nov. 20' 8° a.m placed in sol. 4 to 1000 & examined 27° on 23d 11° a.m.

Certainly some cells, apparently epidermic are quite filled with brown matter in which granules could not be seen, & which alternated with colourless cells in rows— (In all instances in which I cd trace these root-hairs arose from the colourless cells —) In one place all one side of root consisted of dark brown cells & here granular matter cd be seen, which had collected chiefly at one end of cell. — In the pale brown cells without visible granules, the alternation was certainly not due to shrinking of utricle.— In some placed little brown spheres.— I cd see no endoderm brown cells. — yet rootlets so transparent the vascular bundle distinct. —

(3

Urtica

Nov. 23d

Roots kept in water & mud for 24° & placed on Nov. 20th— 8° 20' a.m in C. of A. (4 to 1000) & examined to day 23d about 11° a.m. the same as in last page, but reexamined, fresh, specimen on same day; though later. A fine rootlet near tip, 2 rows of brown cells often adjoin one another; going up rootlet all the brown cells disappeared & still then reappeared; Utricle sometimes a little shrunk, but this not cause of the alternation of white & brown striped. — Wherever I could trace origin of root-hair it was from colourless cells. — Thicker rootlet root, have for a space all the cells were brown, but further along root, there was a space where brown & white rows of cells regularly alternated — Another fine root beautiful alternations— In a thicker rather thick root, I cd see granular matter in the brown cells & here I counted 6 longitudinal rows of brown cells alternating with 6 rows of colourless cells. There can be no doubt about Alternation & I believe all these cells form the epidermis.)