38

[Sept 11th 1880]

Trypsin

At 4.30 P.m put leaves in sol. of Trypsin without Thymol — (for plain sol. of Thymol killed leaves (see Thyme leaves) in few hours, made veins reddish & upper part above immersed part blackened.) At 8º a.m

12th Hazel leaves discoloured — at 10º 30 (ie after 18h. immersion F. examined & found starch dissolved in cells round stomata in the Lime leaves — At 2º Pm Hazel leaves much discoloured — vein reddish — do cells darkened along veins above immersed part of Lime leaves [illeg] in little square petioles.

(All that I shall be able to show in that suction of worm acts like a poison & dissolves stomach — probably acts like a poison by killing protoplasm, as it dissolves fibres — all about colouring of veins & darkening of upper part, we see, goes for nothing

39

Sept 13th

8º a.m Young cherry leaves though the not sensitive of all the Thymol not more affected by Trypsin if but [paper] than the young & old Ivy-leaves —

The fact of Trypsin acting on albumen matter & discoloring leaves (without any Thymol) is important as the killing of the protoplasm in the cells of leaves drawn into worm-hole & consequent great discoloration of leaves is explained.