38

[Sept 11th 1880]

Trypsin

At 4.30 P.m put leaves in sol. of Trypsin without Thymol — (for plain sol. of Thymol killed leaves (see Thyme leaves) in few hours, made veins reddish & upper part above immersed part blackened.) At 8° a.m

12th Hazel leaves discoloured — at 10° 30 (ie after 18h. immersion F. examined & found starch dissolved in cells round stomata in the Lime leaves — At 2° Pm Hazel leaves much discoloured — vein reddish — do cells darkened along veins above immersed part of Lime leaves [illeg] in little square petioles.

(All that I shall be able to show in that suction of worm acts like a poison & dissolves stomach — probably acts like a poison by killing protoplasm, as it dissolves fibres — all about colouring of veins & darkening of upper part, we see, goes for nothing

39

Sept 13th

8° a.m Young cherry leaves though the not sensitive of all the Thymol not more affected by Trypsin if but [paper] than the young & old Ivy-leaves —

The fact of Trypsin acting on albumen matter & discoloring leaves (without any Thymol) is important as the killing of the protoplasm in the cells of leaves drawn into worm-hole & consequent great discoloration of leaves is explained.