[112]

March 4th 1882 Clarkia Elegans

9 pods of both kinds from pots in which single plants grown & 1 pod of both kind from crowded pots — seeds all carefully removed & cleaned.— Only pods with double white thread selected as these were the most carefully fertilized.

The seeds from the 10 pods black thread, (ie fertilised by by pollen of longer red anther of exterior whorl) are rather browner while those of pods with dark white thread, (fertilised by pollen of shorter interior whorl of anthers stamens on road to abortion) are rather darker coloured but only a shade, of really so. Herewith compared & thought so as I did. — I can by eye perceive no difference in fineness or number of seeds, if any difference black threads look finest seeds

N.B Both lots cross-fertilised with pollen from distinct individual.

Weight of seeds (Black thread ie by best pollen) — 361 m gr

― ― (Double White Thread. ie by pollen to be aborted) — 309 m. gr as 100 : 85.6

361 [-] 309 [=] 52 m. 5.2 m gr

[112v]

(Clarkia)

Of the Black White -thread seeds (ie the best poorest & most least numerous 30.9 m gr. gave 59 58 seeds s. or average number per pod of worst kind

as 309 : 361 :: 58 : x - number of seeds in pod of best kind

x = 67.7 — say 68, so on average the pods of pollen from longer stamens have nearly 10 seeds more per pod.

(Seeds placed on damp sand on March 20th — some few germinated on 21' & planted on opposite side (4 on each side) — on 22d more so planted. It is now pretty clear that the radicles more protruded from the black threads (ie longer stamens) than from the white thread seeds— 5 Pots then planted & remainder of seed whether or no germinated in rows on opposite sides.)

(23d This morning a marked difference in the state of seedlings — a dozen of those from longer stamens (black thread) with erect hypocotyls not one on opposite side with white thread)