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Seedlings from Cowslip. — Polyanthus crossed in 1861.

March 7 1863

No. 6. (1 Pencil) a Long-styled Plant, a magnificent & vigorous Homomorphic seedling from long short short -styled flower: now 5 flowers crossed homomorphically with own-form pollen, Black thread — (see Back) & 4 flowers crossed heteromorphically with pollen of Polyanthus out of garden, with

White thread.— March 22d. 5 black & 2 white double threads to mark date. All old flowers still quite fresh like hybrid — refertilised all with same class of pollen.

Another plant of No 6 (2 Pencil) which is short-styled, homomorphic seedling from short-styled plant. (see Back)

1 black thread & 1 white thread as above March 23 3 Black & 2 white: double thread to mark date did not refertilise old flowers, which seemed fresh.

No. 5 (3. Pencil) a long-styled Homomorphic seedling from long-styled by own pollen. — 4 flower black thread own pollen (3)—: 2 white thread by pollen of (Heteromorphically) of garden Polyanthus.

May 14th The whole umbel rotted off. But then no pods had formed

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No 5. March 22d 1 Black & 1 white double thread to mark date (Pencil 2.) Pencil Mark (3) — flowers still fresh— refertilised other flowers, but some few had pistil stigma withered & could not be effectually refertilised. —

No 6. (pencil 2) March 27th 2 flowers heteromorphically with pollen of Cowslip white silk.— 3 flowers homomorphically with own pollen black thread.

March 28th Pencil 1. no. 6. fertilised heteromorphically with pollen of cowslip 5 flowers, marked with white silk. (N. B. all flowers thus fertilised on March 7 quite fresh!!)

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Primroses—

Long-styled 4 flower white thread crossed heteromorphically (12 pencil) with pollen of garden Polyanthus. 5 black thread homomorphically (1 I believe dropped off accidentally) with pollen of polyanthus. April 2d. 3 flowers white silk heteromorphically & 3 black silk Homomorphically with pollen of wild pure Cowslip

Short-styled 4 flowers white thread heteromorphically (no 13 & 14 pencil) with pollen of polyanthus 4 flowers black thread homomorphically with Polyanthus pollen.—

April 2d. 3 flowers heteromorphically white silk; 3 flowers homomorphically black silk with pollen of wild cowslip.

I think all these moderately secure from own-pollen; but certainly not quite so = each capsule ought to be sowed separately =

March 24th. I gathered all flowers, which were open from all 4 plants to see, if both either forms are self-fertil by Thrips.—

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March 22d. 63

I have 7 seedlings; 3 are long-styled & 4 short-styled — to show proportion of sexes

March 23d six short-styled flowers, Heteromorphic from Lon-styled on 3 plants fertilised heteromorphically with common Polyanthus pollen & marked with white thread

Two long-styled flowers on 2 plants fertilised with do pollen heteromorphically marked with white thread.

April 1st 4 flowers of long-styled by heteromorphic pollen of wild Cowslip. white silk

23d Five flowers of above Heteromorphic on 2 1short-styled plants fertilised heteromorphically by pollen of homomorphic long-styled seedlings—; no 6 this is to test whether the pollen of such Homomorphic seedling is good in itself: I looked carefully & am nearly certain no pollen was on stigmas. Black worsted.

Ap. 8 White wool. 5 fl. of the above Heteromorphic long-styled heteromorphically by pollen of short-styled heteromorphic seedling; & 4 flowers of a short-styled plants heteromorphically by pollen of long-styled seedling heteromorphic plant

N.B. 7, 8, 10 Pencil (& Hot-House Plant) are short-styled 9, 11 & Plant which was not covered by net Pencil are long-styled} Heteromorphic seedlings

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March 24 — 63. Common Primrose, potted last autumn & kept out of doors now in flower — is properly long-styled; (No. 15 Pencil) but the pistils are considerably shorter than they shd be, & in almost all the flowers there are 3 with stigmas but little monstrous — The styles towards base became slightly membranous, & then expand & unite & form walls case of ovarium; but they are united in the upper part, & consequently when pulled a little asunder, without any tearing, you can see right into the ovarium & touch the ovules. — The spherical projection within the ovarium crowded with ovules: instead of as is normally the case sending up a thin pointed style needle up middle of common style, is smooth on summit.

One spiral vessel goes up each pistil; but

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I think there are intermediate vessels in case of ovarium.— One had 4 pistils

March 25th 24 2°. 45 — 3 young flowers which had opened not cut off stigmas & part of style; closely examined stigmas not a grain of pollen above on them. — Red wool. —

March 26, 12°, put on heteromorphic pollen (& bound up flowers) & whole another into crown of ovarium, & put plant out of doors under net—

March 28 11° (ie 47°) no pollen-tubes emitted or possibly one or two short ones; but none near micropyle of ovule. —

April 1 no pollen-tube.

April 11th. 3° P.m. opened last pod — now plenty of grains had emitted tubes, which clung to ovules & I am almost certain penetrated them, when they had fallen on chalaza, & not one had entered the "orifice"

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March 27th 63. Homomorphic. Polyanthus-cowslip. no 6.

Long-styled seedling (Pencil No 4) — 2 fl., white silk, heteromorphically fertilised by pollen of wild cowslip—:4 fl, black thread, homomorphically fertilised by own pollen— — N.B. many of stamens on this plant, brown & shrivelled & without good pollen.—

N.B. Of the 3 Homomorphic seedlings from long-styled all 3 are long-styled. like Chives Of the 3 Homomorphic from short-styled, 2 are long-styled & 1 short-styled!

April 8th 7 Homomorphic seedling long-styled (no 5) from long-styled (Pencil No. 5)

Crossed heteromorphically with pollen of short-styled no. 6 (ie homomorphic seedling from short-styled) & marked with black silk thread 3 flowers

crossed heteromorphically with pollen of wild cowslip 2 flowers — white silk. Thread

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April. 14. Homomorphic seedling No 5 (pencil 6.) 5 fl. heteromorphically crossed with pollen of homomorphic seedling no 6 pencil 4? Black thread — 5 fl. crossed heteromorphically with pollen of cowslip white thread

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April 8th I find that the Heteromorphic seedlings which had flowers long & short-styled fertilised on March 22d are now all thoroughly withered & have been withered for some little time — Whereas the Homomorphic seedlings which were fertilised on March 7th are still quite fresh, though fertilised 15 days previously yet but they were fertilised homomorphically. — They may be called everlasting; now in full flower flower for more than a month. —