(1 (X (1

Jan 30' 1862 P. Sinensis

8 Plants raised from all seed of Short-styled by own-form pollen, crossed last Spring. These plants are decidedly more dwarf (though 2 pretty fine) than the 3 other lost. (The long-seedlings from long-styled by own pollen, not dwarfer than the heteromorphic. seedlings.)

Of these 8 2 are now in have one flower just open, whereas in all other 3 larger lots, only one white flower shows bursting petals: hence rather more precocious from dwarfing. Does not Gartner say that Hybrids are more precocious— if so good I think so. —

[in margin:] (The precocity is very little)

(All Used)

Jan 31. Examined first opened pollen short-styled fl. of Homomorphic Union no. 4— (see to roughness of stigma) (1.4) one anther just naturally opened — plenty of pollen. each grain like grain of disc — swelled in water (& I believe shortened??—) but in all I could see within granular matter & a nucleus!!! & length of each distended grain 11 1/2 / 7000 & breadth only under 8/7000. — I do not remember such proportions.─

[1v]

Sticks mean Short-styled

Black thread or worsted means Homomorphic union

White thread or worsted means a Heteromorphic union

Thread goes with Sticks

Worsted with no sticks

I crossed 4 flowers of no. 4 with perfect pollen from the Heteromorphic seedlings for comparison & marked with white worsted or thread, mixed with red worsted or thread. red & black worsted.— I suppose these were crossed Homomorphically, but no record made

(2

Feb 5. seedling short-styled from a Heteromorphic union (viz long-styled by short-styled pollen) Nuclei visible — The grains distended are oval.

seedling short-styled from a Homomorphic Union (viz no 4.) — (Plenty of pollen, as much as in the above Heteromorphic seedling & in apparently exactly the same state & appearance of goodness. ―

Feb. 12 1862 I fertilised the seedlings from the Homomorphic union of 1861. No 4. Zinc label (ie from short-styled by own-form pollen) with pollen from seedlings from same Homomorphic union; as I expected these to be sterile.— (over)(PP)

I crossed 3 with black thread or worsted & 2 with white string thread or worsted

Feb. 21 — 3/6 — Homomorphically — 4/6 Heteromorphically

For comparison I crossed but Homomorphically & Heteromorphically seedlings from the Heteromorphic union of 1861, as these would of course be fully fertile.— 4 with black & 4 with white threads

(over) zzz

[2v]

The long & short stamens are affixed in line of midrib of petals—

There seem about 10 ribs or carpels on ovarium?—)

P.P. Feb 27 crossed 2 more homomorphically & 2 more of no. 4 heteromorphically: so that now 8 of no. 4 are crossed both ways, making 16 crossed

zzz

Feb. 23 I crossed 12 more flowers (i.e. 20 flowers altogether) of the heteromorphic seedlings (no 1 & no. 2.) crossing 10 homomorphically (ie black thread or worsted (according to sticks) & 10 heteromorphically with white thread or worsted. — (This experiment will serve to test last years experiments)

(3

Chinese Primroses

Feb. 21. 21st —1862

Homomorphic seedling. (no. 3) (from long-styled plants with own formed pollen.—)

I crossed

All these plants    in number are long-styled!! Their pollen seems good.

I crossed 4 homomorphically with own own form pollen marked black thread worsted

Feb 24 I crossed. Six more (making 10 altogether)

And for comparison I crossed 4 of no. 3) heteromorphically with perfect pollen from the Heteromorphic seedlings. No 1 & no. 2 marked Black with white & red worsted. — To test with stigmas of no 3 are perfect

Feb. 24 And for comparison I crossed 4 (flower of no. 3) homomorphically with perfect pollen from the heteromorphic seedlings no 1 & 2. — Marked with Black worsted & reddish string. — To test whether stigmas of no. 3 are perfect

[3v]

(No. 2 are Heteromorphic seedlings from long-styled by pollen of short-styled)

(No. I are Heteromorphic seedling from short-styled by pollen of long-styled plants)

(No 3 & no 4, though the latter at first clearly dwarfed, & even in slight degree rather precocious in flowering, were not precocious, but rather late in setting their seed.)ß May 29. 62

to test whether pollen of no 3 be good  →

(4

Feb. 24th. Crossed 4 long-styled flowers of Heteromorphic seedling (1 & 2) with pollen of no 4.  crossed them Homomorphically, Heteromorphically & marked with black white thread wor thread mixed with black white worsted. As these were long styled & were not open castrated, can be in some degree trusted in testing power of pollen of no. 4

Crossed 4 5 flowers of Heteromorphic seedlings short-styled (no 1 & 2) with pollen from no 3 —crossed then Heteromorphically Heteromorphically Homomorphically & marked with white thread ringed with white worsted & red string

As these short-styled flowers had to be opened to be fertilised, some of own pollen probably got on, & so power of pollen of no. 3 can hardly be inferred with safety from this experiment.— (Useless indeed perfect pods are produced.)─

[38]

Feb. 28th

Of no. 1. (Heteromorphic seedings ie. from short-styled flowers by pollen of long-styled) there are 10 plants, of which 5 are short-styled & 5 long-styled, yet in flower

Of no. 2. (Heteromorphic seedling i.e. from long-styled fl. by pollen of short-styled) there were 9 plants, of which 6 are long-styled & 3 short-styled + 2 = 5 short-styled

(2 more have to flower) →

April 23d. one more has flowered & this is short-styled.

July 26. do do do ― is short-styled

Proportion of form in Heteromorphic seedling

(6

Feb 27 1862 In the 2 large pots of Homomorphic seedlings no. 3 from long-styled by own-form pollen: there were nine plants, all long-styled; besides others not yet in flower In the separate pots there were plants all long-styled.—

(zz) One of these plants had stamens as in long-styled & stigma as in do, but the pistil was short, & stigma stood just above level of anther instead of far below; so that this may must be counted & makes 10 long-styled as mid-styled (see Supplement p. 6.)

Nine plants of no 3 in separate pots are all long-styled — (one has not yet to flowered this will die) All used except about mid-styled

March 14th another plant flowered in large pot, truly long-styled, which makes 10 (not counting the approximately mid-styled). (Add the plant which I thought was "mid-styled" formerly.)

Of no. 4 (ie short-styled by own-form pollen) there are six 7 plants, of which five are short-styled (2 in pots have not yet flowered,) & one long-styled, (which is not bigger than the others)

July 8th another has flowered & is short-styled.

[6v]

Feb. 28. I have no hesitation in saying that 6 Plants of no. 4 now in full flower, are dwarfer on average than others "& had a slight tendency to precocity in flowering"

Two ho plants have not flowered & this occurred with very few of many other seedlings raised.

(No. 4 seedling from short-styled by own pollen)

(5

Feb. 27. 1862 Crossed flowers of no. 4 heteromorphically 2 with perfect pollen of no 1 or 2 & marked with red Braid worsted, white worsted & white thread

Feb. 27. Castrated 2 long-styled flowers of no 1 or 2 & fertilised with pollen of no. 4, to see if pollen of latter good marked with white Worsted thread Black thread & red worsted Braid. —