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March 1st: (62) Mr. Turnbulls Ch. Primroses

There are 29 seedlings raised from a plant of Mr Ducks of these 20 are long-styled & 9 short "mid -styled;" but strange to say the latter have n none of them the proper long-stamens pistil? & therefore resemble the one plant of no. 3 (p. 6) — viz "mid-styled"

There are 17 seedling raised by Mr Horwood from 4 thrum-eyed plants, by themselves on shelf he feels sure, & all these are "mid-styled." as Horwood calls the latter thrum-eyed, the parents may have been mid-styled.

He did not fertilise them, so probably they are result of Homomorphic union & this countenances the Homomorphic

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P. Sinensis is apparently "trimorphic"

Mr Horwoods seedlings were blotched, giving an idea that they had been produced from some cross??

The are seed came from Dobsons? — [illeg] as, wh. do not fertilise, would naturally select this true Hermaphrodite form, from its producing so much seed.—

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seedlings having same inherited form.—

But are these mid-styled plants "moprhic" at all? I shall partly know by how they seed.—

Four mid-styled Horwoods seedlings had globular stigmas, like the short-styled. The stigma rises just to top of anthers & could easily get dusted by own pollen. The anthers are low, so the pistil ought to be long-styled & stigma ought to be elongated.

March 3d. I have crossed 6 flowers of these Horwoods seedlings with pollen of my heteromorphic long-styled seedlings, marked white worsted, & these ought to be most fertile; for the stigma of Horwoods seedling are globular like a short-styled stigma so this cross would be heteromorphic.

Enough did not set

I also crossed 6 flowers with pollen of

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of my heteromorphic short-styled pistil & marked with black worsted; & these ought to be less fertile, for on above view it would in fact be a homomorphic union. — As pistil of these Horwood seedling is almost surrounded by own anthers, hardly possible to make fair cross, but I delicately brushed over each stigma, after splitting & removing the corolla.

March 4th. "Ducks seedlings", from Mr Horwood — first for long-styled, the stigma does not project quite so much out of the flower (I have looked again & I think this hold good) as in my regular long-styled flowers; & we have seen that out of same lot of seeds, 290 were of this character & 9 were "mid-styled"; in those long-styled, stigma somewhat elongated but not rough like my long-styled. The stigma of of Ducks "midstyled" is globular & not rough: from

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8 spiral vessels in my short-styled, close to stigma.—

9th (or 10?) in some mid (?) & long-styled

(March 6. Sir J Lubbock 16 plants. (but many previously thrown away) all long-styled!)

(Parslow looked at Cattells plants only 6 left, 1 long-styled & 5 short-styled)

These agree in shape of stigma & roughness each with proper type. —)

"Cattell, John, 1786/7-1860. Nurseryman of Westerham, Kent. CD often ordered seeds and plants from C. Many records in CD's Account book (Down House MS). 1860 CD to Maxwell Masters, the nurseryman CD generally dealt with. CCD8:147." (Paul van Helvert & John van Wyhe, Darwin: A Companion, 2021)

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these facts I infer that all 38 29 are really long-styled, but that 9 have had pistil much reduced in length, so as only just to project above anthers; & the stigma has consequently become more globular & smoother: it will be curious to know what its function is.

One of these flowers, which had not fallen off had its stigma covered with pollen-grains, which had emitted their tubes.

Horwoods "mid-styled" seedlings — the pistil is decidedly longer than in my true short-styled; so that again & stigma globular & not rough; generally short-styled so this is probably long-styled with reduced pistil??

The stigma stands just above anthers, which are seated low down, exactly at the same height with short-stamen of long-styled flowers.—