[94]

Sep. 20 1868.

Borreria nov. sp. near B valerianoides (This genus not near to Hedyotis)

Rubiaceous plant from Fritz Müller.

Long-style – pistil 3 times as long as short st. with stigma a little larger & the 2 lobes more divergent. The anthers stand low down in tube of corolla & are hidden.

Within At the mouth of the tube above the anthers numerous beaded threads almost fill up the mouth of the corolla & project above it.

In short-st. the stamens project above the mouth of the c.; & the stigma has hidden in the tube. The beaded threads occupy in this form a very different position for they are seated low down in the tube beneath the attached filaments of the stamens

[94v]

Stamen similar in both forms.─

Much nectar

Corolla of 2 forms alike

Some of anthers of Long-styled contabescent & in other a good many of pollen-grains shrivelled.

(From widely different & changed position of branch of papillæ in the 2 forms we base their importance)

Sept 27 68. I have again examined pollen & no mistaken though pistil so different in length very little diff in size of grains ─ When Dry that of short styled 9-10/7000 & a shade larger & broader than that of long-styled ─ When rendered spherical by water that of short-styled 12/7000 in diameter & that of long-styled hardly more than 11/7000 in diameter.

[95]

Hence beaded threads in each case form stand near stigma & we thus learn their function, viz to receive any loose pollen from an insect, & afterwards place it on another, for in the long-styled form the threads stand above anthers & cannot catch spontaneously falling pollen. It is very odd seeing great difference of length of pistil, that if no mistaken─ the square pollen-grains of Dry pollen

[data not transcribed]

[95v]

The beaded [2 words illeg] collect pollen from all parts of body of insect & then replace them as body opposite the stigma.

(I raised 10 Plants ─ 5 proved Long-sty & 5 short-styled.)

(Jan. 11 69. I smeared 3 heads of long-styled flowers with pollen from short-styled, & these have produced dozens of capsules, even with 2 seeds. I did not attempt from smallness & crowding of flowers to fertilise them separately. I also fertilised separately 2 Heads with pollen from another long-styled plant, but they did not set one; nor did whole plant spontaneously. On a 2d long-styled plant, here & there, a single 5 or 6 early capsules were produced with seed, but as this plant stood in a short tube near be a short-styled plant many early in season have received a few grains of pollen.─ (I had better stick to the one good case.)

(Feb. 11. The long-styled Plant has now set some seed & which have been produced so late that cannot be due to cross, whist Kept in other Hothouse ─ is not quite self-sterile.