[7b]

Down. Nov. 26 1860. Careful Resume of effect of Plain Water

(I was not the sure of action on Long Heads)

Inflection 1 leaf, in 1° 45' / 2 leaves in pure water, so I suppose not distilled; in 2° / in 4 spe. in 3° / in 4 spe. in 3° 15' /

: (As these I believe were not in distilled water, must not be trusted)

Changes of contents of cells in whole leaf placed in Water

1 in 3° 15 (but leaf not previously examined) a trace of sphere behind L.H. a very few spheres beneath round-Heads.

1 1° 42' no change in cells beneath round Heads- no spheres formed behind Long Heads

2 2° no change do

3 2°  do d

4 2°  40' do d

5 3° do p 26 one case omitted

6 - 3° 15

7 8 9} 3° 15' do do

10 11 12} 4. 30' do do

13 6° 15' do but submarginals with red somewhat broken

14 16° 30' do (Heads blackened.)

15 21° 15 do (Heads blackened.)

16 16° two of breaking up below round Heads.

17 17° two of do do in 41° much broken & few spheres behind L. Head

18 41°

Used

Hairs cut off & placed in pure water between slips of glass & examined before & after.

1 in 46' no change

2 3} in 1°.  16' no change

4 1° 56' no change

6 Hairs 3° no change.

10 altogether

[line illeg] p 21. p 22

[in margin:] May be trusted for under 6°. Try more specimen for 6° or 7° in water