[124]

Aug 4th

Sugar

8º 59'. 1 leaf ([illeg]) in 30 m of sol. of sugar 2' to 1 oz. — (9º 3' added 30 minim of C. of Amm 2 gr to 1 oz) 9º 5' gland blackened

(9º 10' strong inflection) (Thus even 2 gr of sugar to 1 oz does not prevent action of C. of Ammonia 2 gr. to 1 oz)

(C. of Ammonia)

[123v]

[Draft of Expression, published, p. 100.]

(O

Aug 4th — put on at 10º 39' minute drop of sol. of sugar (2 gr to 1 oz) on 7 glands— added particles of meat & glass at 10º 59' (ie after 20')

11º 3' 2 meat meats & 1 blue-glass have curved well in)

12º 3 other glands with meat & 1 with blue glass have not curved in; as leaf is so active, I think this retardation must be due to sugar.

2º 5' At last 2 latter glands with meat, & 1 with blue glass have curved in a little, but not the one last with meat: I remember that I put a good deal of the sol of sugar on these 4 latter glands, & the sol. certainly delays delays action.

(Also put on other glands on same leaf particles of meat & glass at (leaf very active for 4 or 5 other glands soon acted) with glass & meat.)

Minute drop of sugar

[126]

Sugar

4. 8º 19' 4 leaves in 2 dr of sol. of sugar (1 gr to 1 oz) (but kept in for 10')

8º 29' added 2 dr of Ph. of Amm (1 gr to 20 oz)

(11º 30' beginning to inflect)

(2º 10', consid. inflection in 2 leaves, & a little in 2 others, but hardly more in latter than water sometimes causes)

(4º 30', 2 leaves with laminæ inflected, consider inflect in other two leaves

I am not quite sure that I did not blundered in quantities

[127]

Aug 4

Sugar

Aug 4 2 leaves (plain cup) with 1 dr. of sol. of Sugar (2) (2 gr to oz) at 8º 45'.

8º 50' added 1 dr of Phos of Amm (1 gr to 20 oz)

((9º 37 put on a fresh leaf with long stalk; so that this leaf had not been previously exposed to the sugar sol & at 10º 30' was slightly inflected;) but not so much as might have been expected)

(11º 5; one of other the 2 leaves now moderately inflected.

(11º 30', the other a little inflect) (2º 10' both leaves now much inflected, one greatly & the laminæ of both) This certainly look as looks as if morphia caused more retardation than sugar, & we observe one gr of salt of Potash causes no delay)

(I am not quite sure that I did not blunder in quantities)

[128]

Aug 3d

8º 18' 3 leaves immersed in sol of Sulphate of Potassium (1 gr to 1 oz) not washed, taken out & soon dusted at 8º 55' to 8º 58' ie .39' — I merely merely drained off the potash & stood leaves upright — (9º 2' meat meat acted on some 2 or 3 tents.)

9º 15', some inflection from glass, & from meat in all 3 leaves, about equal from glass & meat) (9º 35', the inflection is not as yet much (10º 25' plenty of inflection in all 3 leaves from both meat & glass)

(11º inflection from glass & meat nearly equal — in 1 leaf enormous, & in the other 2 tent very strongly marked on both sides)

(P.

Aug 25th 11º 6' put in 4 leaves in 2 dr of sol. of sugar 2 gr to 1 oz. Leaves rather old)

at 26' ie aft 20' added 2 dr. of sol. of Ph of Amm 1 gr to 20 oz

For some time no action at 3º great inflection in one & some in others.

3º not so much action as below, but now considerable inflection for some have previously no action, not so great as in cases below)

do in pure water at 11º 10'

at 11º 26' added 2 dr of [illeg] Ph of Amm 1 gr. to 20 oz.

Also put in 2 leaves (with pins through stems)

11º 33' some action — (at 3º great inflection in 2 leaves; some on other 2)

Ph. of am 2 dr. (1 gr. to 20 oz) & 2 dr. of sugar. simultaneously 2 gr. to 1 oz at. 11º 28'. — 11º 23' some action — (3º great action in 1 leaf some in other

/over

[Pv]

Leaves not good enough to form final pigment, but I certainly think sugar delayed action of the Ph. of Amm.

Try dusting with glass on one side & with meat on the other for this ought not to delay action of pressure, after 20' in sol. of sugar.)

(Aug. 26th 9º 30; It is certain that sugar mixed with the Phosphate does not stop the most energetic action; & as 2 of the leaves which were first put into sugar & then into the Phosphate are now well inflected with the laminæ, & the 2 others fairly inflected, & as they did not become so soon, I believe that sugar does delay action of Ph. of Amm. — These 4 leaves are now about as well inflected as those which were first immersed in water for 20' & then given the Phosphate)

[130]

2 leaves 2 dr. of sols. of sugar & Phos. of Amm. together as above at 8º 27' — 8º 32' some inflection in one leaf — (8º 34' decided) 8º 45' both leaves moderately well contracted

Sept 8th p. 17

2 leaves in plain water for 20' & then Ph. of Ammonia for comparison at 8 28' — added Ph. of Amm at 8º 47'

8º 58' decided, but very slight inflection — 9º 10', & 10º. 10' still very slight inflection 11º 40' fairly well inflected. 1º strongly inflected & lamina of one leaf. (over)

[131]

Sept. 7th. 8º 25' — to 32'. for all following sols.

3 leaves in 4 dr. of sugar 2 gr to 1 oz water

(At 8º 47 50', to 55' (ie. after full 20') added 4 dr. of Phosphate of Ammonia & the same to all the others; having first washed them in water.

9º 20' a trace of inflection — (9º 30' do, chiefly long-Headed) (9º 40', sugar-sol more effect acted on, than in the Potash dish) (10º there certainly is more inflection than in the C of Potash specimens) (11º 20' no more inflection & there is extremely little) 12º 25' inflection more pronounced in 2 of leaves

(2º— one L with lamina & all tents inflected; 2d leaf with most tent inflected; 3d with a few inflected)

3 leaves in do of gum 9º. 2' some inflection in all 3 leaves

9º 5' decided action (9º 15' fairly inflected) (11º 20' there ga are rather more inflected than the water specs)

2º all tents well inflected)

(4º 45' closely inflected) like those in water)

3 leaves in do of Cab. of Potash

12º 25' a trace of inflection, chiefly in one leaf

3º one leaf with lamina & all tents inflected; 2d leaf with most tents inflected; 3d leaf with a few

(2º all 3 leaves with almost all tentacles fairly well inflected

(4º 45' now well inflected

17

Sept 8th

3 leaves in sol. of sugar. 2 gr. to 1 oz for 20'

put in at 8º 24' & after 20' given C. of Ammon 2 gr. to 1 oz. (old sol.) — added C. of Am at 8º 45' Glands all blackened in 2 or 3 minutes — at 8º 52' [-] 45 [=] 7 considerable inflection of tentacles 8º 55' great inflection.

C. of Ammonia

17

8º 25 Sept 8th

3 leaves in Alcohol (1 dr. of to 7 dr. of water) for 20' & then at 8º 25' given Phosphate of Amm 1 gr. to 20 oz

added Phosphate Amm at 8º 45'. At 11º 5' one leaf with perhaps a trace of inflection — kept in nearly same state 2º. 15', when more inflected & a 2d leaf had just begun to contract. At 4º the lamina of first leaf was inflected & many of the tentacles of the 2d leaf inflected. The third leaf not inflected at 5º. — (6º 45' the 3d leaf very slightly affected)

It is clear this weak alcohol delays inflection.

[133v]

Sept 7th 8º 45' put 3 leaves in distilled water & no inflection occurred

Sept 8th 8º 35 added plenty of Phosphate of Ammonia (1 gr. to 20 oz) & left for 48º & no inflection occurred, except on second day, slight in 1 leaf. — Thus immersion for 24º in pure water stops action of Phosphate of Ammonia