138

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   Abstract of old & new Observations, Phosphate of Ammonia (see Back)

page    First for old I think, 1872. end of Sept & Oct.

No of Leaves

59    1 leaf 10 minims of 1 gr to 24 oz, so the leaf got 1/1152 of gr.

+ 1

   after 9' plenty of inflection; after 14' immense; after 2. 37' all but 7 inflected

   ——

   (two leaves with do in one vessel)

   after 16' a good deal of inflect; after 6. 25' all except 10 tents; kept 10 for 24°

+ 2

   Second leaf — very little affected till evening

- 3

60    1 leaf 10 minims of gr. to 30 oz; so got 1/1440 of gr.

   after 22' some inflection; 2. 15' very consider. inflect; after 3. 45' great inflection; increased    next after 23. reexpanding

+ 4

   1 leaf with do.

   after 42' all more or less inflected except 3; after 2. about 10 not closely & 5 not at inflected

+ 5

  1 leaf. with do

   after 36' only 14 not inflected; remained in same state

+ 6

60A    1. leaf 10 minim of 1 gr. to 36 oz so each leaf got 1/1728 of gr.

   after 45' almost all  inflected: no. after 1. 47', about 28 not inflected

+ 7

   after 38'    do.  after 1. 35' all more or less except about 7 t. l after 3. 20' all but 4

+ 8

   1 leaf 10 m. of 1 gr. to 40 oz.; so each leaf got 1.1920 of gr.

   after 1. 5' a vast number inflected; after 2. 5', from 25-30 to not inflected

+ 9

   after 55' all except 3 inflected, & remained for another 1.

+ 10

60B    1 leaf 10 m. of 2 gr. to 50 oz, so each leaf got 1/2400 of grain

   after 9' great inflection; after 54' all but 3 strongly inflected & lamina.

+ 11

   after 7' grt inflection; after 57' all but none or inflect & 3 only sub-inflected

+ 12

 

do    1 leaf 10 minim of 1 gr. to 60 oz, so each leaf got 1/2880 of gr.

*    after 17' immense inflection, all but 4 & 5 not quite close; after 1. 35', all but 3 & lamina

+ 13

 

do    1 leaf to 10 m. of 1 gr to 80 oz (2 leaves in 20 m.) so each leaf got 1/3480 of grain

   young & colorless fine red leaf, after 3' some inflection; after 1. 4' all except 2, but 15 not closely; after 1. 30' every tent *(2) closely except 1.

+ 14

   white leaf after 8' consider. inflection; after 50' 12' grt inflection; after 1. 20' all but 20—40 inflection; after 4º 15 all but 17.

+ 15

 

60C    1 leaf to 10 m. of 1 gr. to 100 oz (2 leaves in 20 m.) so each got 1/4800 of gr.

   after 10' consider. inflected; after 44', all except 20 t. inflection; after 1. 34' all except 11 t. & so remainder remained so for 21.

+ 16

   after 10'    do   ;    do    all except 29 t.; after 5. 46' all except 47 t. remained so from 24º

+ 17

60D    after 12' all inflected except 2 & 3 00 & so remained for some time

+ 18

 

60D    1 leaf 1 dr. to 200 oz, 10 minim, so each leaf got 1/9600 of gr.

   after 10' strong inflection; all except 11 marginals; after 24 all except 6 & 5 sub-inflect remained so for some time

+ 19

 

60D    1 leaf 10 m. of 1 gr to 400 oz. so leaf got 1/19200 of gr.

   after 9' some inflection; after 1. 9' all more or less inflected except 19 t.

+ 20

See source of error back

138v

These experiments with only 10 minim not secure because I had to push leaf about — see corresponding water experiments?

p. 60 E

I refer to these at back of Page C

*This leaf had 235 less 3 = 232, so each gland got 1/668,160 of a grain

*(2)    176    176 ―    1/675,840

Corrected by Emma & one done by George so trustworthy

139

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   new Abstract    Phosphate of Ammonia July 1873 Best Work

Page

   1/2 dr of 1 gr. to 100 oz, so each leaf gets 1/4800 1600 of gr. of the salt

1    (1) after 10' grt inflection; after 50' all perfectly inflected; after 8. do; after 23º, trace of reexpansion

+ 1

   (2) after 1. a few inflected, several sub-inflected; after 3. all inflected or sub; after 8. all, except 4 t. after 23. reexpanding

+ 2

   (3) ― 4 inflected      ; ― all except 9 & these sub-inflected; after 8. do; —    after 23°, sub-marginal still inflected, marginal fully expanded

+ 3

   (4) after 54' all inflect except 8 marginals; after 3. all except 4 & these sub-inflect; after 8. do. after 23. trace of reexpansion

+ 4

 

2 4 leaves in 1 dish. 2 leaves old    (1) after 36' almost all inflected; after 4° 35' same state; aft 7. 45'; all except 6; after 24° much reexpansion in all 4

+ 5

   (2) ―    do   ; ―    do   ; all except 5;

+ 6

   (3) ―    some   ; ― all round disc & most marginals subinflected; — as before

؟ 7

   (4) ―    some   ; ― about 30 well inflected & others sub;, as before

؟ 8

 

3    (1)      ; after 3. 10', all except 20 marginals; after 5. 10' all except 15l after 21. all except 3

+ 9

pale (2) after 28' great inflection; ― ― 18 ―; ― ― 16; ― 1/2 reexpanded

+ 10

old (3) ―    great inflect; ― ― 30, ―; ― ― 21; ― all reexpanded

+ 11

very pale (4) ―    mere trace; ― —more traces; ring on disc; marginal sub.; ― responded.

O - 12

 

4 4 in dish red (5) after 15' gr. inflect; after 3°. all but 10 t.; after 5°, all except 1 l after 21. ring

+ 13

red (6)    do   ; ― ― 16 t; ― ― 14; reexpanded

+ 14

red (7)    do.   ; ― ― 16 t; ―    18; reexpanded

+ 15

whitish (8) very little inflc; discals alone inflected; discals, trace in sub-marginals; partly reexpanded

- 16

After 24° the glands not blackened— 16 leaves here were here tried each with 1/4600 1600 of a grain

Of these 12 strongly affected & 11 of them considerably or strongly under 1°; & the 12th to became so after 3. —

Two leaves only moderately inflected, but these had more tent inflected & sub-inflected than water specimens after 4° 35' — two were hardly at all inflected, but both these were very pale & therefore weak leaves.

(As number (1) had all tent inflected after 50'; assuming it had 160 too few tents. each gland could have got only 1/768000 1/112,000 of a grain)

1600 [x] 160 [=] 112000

(Lime Water )

(Water 12 leaves    1 had after 40m 9 tent inflected & 3 other had few — 1 to 3 or 2 inflected..)

16 [+] 16 [+] 2 [+] 12 [+] 16 [=] 62 + 4 during 1874 = 66

140

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New Abstract Phosphate of Ammonia July /73/ Best work

Page    1/2 dr of 1 gr to 200 oz, so each leaf got 1/3200 of a grain

5    (1) after 5' 1. 55', 28 marginals inflected after 4° many inflected l after 8. 40' all except 9.    after 23. all inflected except 1.

(see Back for summary)    ؟ 1

(2) after 48', 58—60 1°, 47 t. inflected; after 6. much inflection, except outer marginals

؟ 2

   (3) O after 2°, about 21 inflected; after 6 only 8 to sub-inflected, & went on reexpading

؟ - 3

   (4) O after 2°, about 19 inflected; after 4° submarginals & discals forming a ring; continued same

؟ - 4

——

6 (4 in dish) (1) after 6' several inflected; after 1. 50' all except 3 or 4 inflected; 3. 45' same state & remained so; after 23°, beginning to expand

+ 5

   (2)    do   ; ― many inflected   ; 3. 45 discals & sub-mrg. forming ring; rexpanding

؟ 6

   (3)    do   ; ―    do   ; 3. 45 30 or 40 inflected; a ring    do

؟ 7

   (4) O       after 1. 50' about 12 inflected — a slight ring on disc

— 8

——

7    (1) after 2°, all but 19 marg. (?) inflected; after 6. all but 22 inflect.; after 9. all but 6; after 24º do, with lam.

+ 9

   (2) ― ― ― 8 ,; ―    7   ; ―    5 or 6; ― almost rexpanded

+ 10

   (3) ― ― but 50   ; ― about 59 inflected; ― reexpanding; ― only a trace

؟ 11

small (4) ―    but 4   ; ― beginning to reexpand; ― greatly reexpanded; ― do

+ 12

   (5) after 1. 20' all but 26 inflect; after 5. 40' beginning to reexpand; after 8°. 30; greatly reexpanded; after 23. ring

+ 13

   (6) after 1. 45' ― but 11; aft 4°. 40', all, but 12 only sub, lamina; after 8. 30', all exact 3 or 4; ― do

+ 14

   (7) ― ― but 7   ; after 5°. 10' all but 2;    do same state; rexpanding

+ 15

   (8) ― many inflect or sub.  ; aft 5°. 40' all but 23, & some of these sub.; all but 16 infl; rexpanding

+ 16

9 & 9A very hot day 8th    1/2 m/ of 1 dr to 300 oz — so each leaf got 1/4800 of a grain

   (1) (pale) after 1. 25' all but 8 inf; after 2°. 40' same state; after 4. 15' same; after 8° 30' rexpanding; 24. fully

+ 17

   (2)    ― ― only 24 inflected; ―    same   ; af ― same; ― all reexpanded

+ 18

   (3) ―    but 5 ;* ― same   ;    same; all outer rexpanded; sub-mrg still inf.

+ 19

   (4) ―    but 3 ;* ― same   ; ― now 6 rexpanded; ― many reexpanded; still ring

+ 20

 /see Back for calculation

   (5) after 9' a good deal of inflection; after 16. extreme marginal alone not inflected; after 1. 50', all except but 23 inflected; after 6. considerably reexpanded; after 23. almost fully rexpanded

+ 21

Summary of upper lot 1 gr. to 200 oz. 1/3200 of oz, 16 18 Best leaves tried; within 2. & sometime within 15' 134 of these leaves were strongly inflected; & 3 4 only slightly inflected & 1 so slight as to be doubtful. viz 21, 19, & 12 inflected, & 1 so slightly as to be doubtful 4 at best & it shd be observed that of 16 water spec. tried at the same time, after from 2. to 12. not one had nearly so many as 12 t. inflected.

Summary of 2d lot of 1 gr to 300 oz 1/4800 of grain — only. 5 flower tried

/Very hot day Of these 5, after 1. 25', all ex tentacles inflected, except in one case all but 24 & sensitive, one had after 1. 40; 7 inflected & another the similar number of 10 ,(but after, 4° to 7. reexpanded;— this goes for nothing, as several of the Phosphate spec, reexpanded in about same time interval, owing I suppose to heat.)

140v

* no 3    This leaf had 236 — less 5 tents = 231

   231 [x] 4800 [=] 1,108,800    so each tentacles got 1/1,108,800 if gr.

* no' (4)    is also calculated at back of red page 9. but none worth giving 1/1,027,200 of a gr    Yes do give it

140Vv

page

   Ph. of ammn 1 gr. to 500  oz,  but only 1/3200 of grain given to each leaf as before, so only difference more diluted.

13 old leaf (1) after 1. 17' a large number, about 80 inflected; after 5. 10' all but 38 inflected; after 8. 10, all but 32 inflected after 23. fully reexpanded

+ 17

palish (2) ― slight ring on disc; after 5. 10', many some marginals slightly inflected; after 8. 10 same; after 23. still a ring on disc

some effect    - 18

141

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   New Abstract Phosphate of Ammonia    July 1873

Page    1/2 dr. of 1 gr to 400 oz; so that each leaf got 1/6400 of gr.

      for Summary see back of p 10

10 all 4 very young leaves (1) after 35, only 2 or 3 inflected; after 1. 10' only 5; after 3. 35' ring on disc; after 8. 10', all sub-marginals grandly inflected; after 21°, all more or less inflected, except marginals

- 1

   (2) after 1. 10', a trace on disc; after 3. 35' outer discals; after 8. 15' ring; after 21. all except margin

- 2

* (3) after 10' consider. inflection; after 32' all but 6; aft 1. 5 all but 4; after 21 rexpanding

+ 3

   (4) after 9' some inflect.; after 35' decided alight; after 1. 10' some sub-mar also inflected; after 8. in many sub-marg, closely inflected; after 24° all except outer marginals

؟- 4

11    (5) after 26' decided trace of inflection; after 1. 4' trace of ring on disc & some sub-marg inflected; after 3. 26' good sub inflection of all sub-marginals; after 8°, all but 2 or 3 t. grandly inflected & lam, after 21 rexpanding

؟ 5

   (6) after 53'. 7 marginal inflected; after 3. 20' a ring; after 8°, all but 2 outer rows grandly inflected & lamina — after 22°, same state

؟ 6

   (7) after 55' mere trace; after 8. a ring on disc — after 22. same state

- 7

*(8) after 13' all but 7 t.; after 55' all but 3 more or less; aft 3. 15', inflecting increased; after 7. 45' lamina inflected; aft 21. same state

+ 8

 

Highest —( these 4 experiments tried with great care) weighed at Chatham.—

12 pale (1); after 10' much inflect; after 2. 30', many marginal & sub forming a ring circle; after 4°. 30' same; after 8. 30 rexpanding

+ 9

pale (2) ―    do, ― vast proprt. inflected   ; ― all except 35; ― greatly rexpanded

+ 10

palish (3) ―    8' much inflc.; ― about 19 well inflected   ; ― same state; ― do

؟ 11

palest young (4) ― 7 some inflect; ― 10 marg. inflc & ring; ― discals a ring; ― rexpanding

؟ 12

N. B I add that in between 21' & 25' after immersion all these Phosphate showed much inflection

Summary    12 leaves tried with 1/6400; many of these young & pale & poor leaves. Of the 12, 3 not acted on, only 2 with almost all closely inflected within 1°; the other seven were all acted on, rapidly to a certain extent, but several of them did not become well inflected till, 3°, 4° 30 even 8. had elapsed. Yet what a contrast with the 12 water spec. only one of these had 13 marginal inflected & those began to reexpanded after 8. 25'; some other had 2 or 3 only inflected/

(See additional remarks are p. 10 red figure)

142

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   New Abstract Phosphate of ammonia July 1873

Page

1/2 dr. of 1 gr. to 500 oz; so each leaf got 1/8000 of grain— This strength by weight is as 1 of the Phosphate to 218,750 of water. In the 8 latter experiment, in this page the Ph. was weighed in Chatham — took special pain in all measurement — & in selecting the finest leaves which had expanded in Greenhouse; & the weather had for 3 or 4 day been very hot 75°—81°. All this applies equally to water & Phosphate experiment.

14 Young & pale. (1)    O    O    O    O

- 1

   (2)    O    O    O    O after 22. sub-inflect of submarginl

- 2

   (3)    O    O    O    O after 22. ring on do

- 3

   (4)       after 7. a ring on disc       after 22. sub-margins sub-inflected

- 4

15 large oldish (5)       after 6. a ring; after 11. 30' sub-marg, considerably inflected after 22 sub-marg. inflection

- 5

   (6)       O   .    O    O    O

- 6

   (7)       O    O    O    O    O

- 7

   (8)       O    O after 11. 30, some sub-marg inflected — after 22. same state

- 8

   (I state that after 11. 40' the Phosphate, certainly differed from water specimens.

16 reddish (1) after 40'. a good deal of inflection; aft 2. 20' many marginals sub-inflected; after 4° 13' fully half the tentacles inflected — remained in same state — after 24°. still slightly inflected

+ 9

   (2) after 39' a large number inflected; 4° 22', nearly half the t. inflected (∴ about    ); after 23. still slight inflection

+ 10

reddish (3) after 39' ― do ― ―; aft 2. 18'', all but 25 inflected; after 4° 17' all but 16 inflected    after 23. outer discals & sub-marginal still inflected

+ 11

   (4) after 30' largish num ― do ―; after 2. 18', about 28 inflected beside discals; after 5. 20' about one-third of all tents inflected; after 8. much reexpanded; after 23. trace of ring

+ ؟ 12

(I remark, that in between 8' & 4' after giving the Ph. a trace of action on all leaves)

17    (5) O after 14' some inflection; after 20', 19 inflected, same, same; after 7. 45' reexpanding

؟ + 13

   (6) — after 13' & 20' consider, inflect; after 2. a considerably no' inflected,— same; after 7°. 45' reexpanding

؟ + 14

   (7) — after 12' a good deal of inflect.; after 2. a large no' inflected ; after 4° 2' all t. inflected except the 2 outer rows; after 7. 40' same state; after 23. reexpanding

+ 15

   (8) after 8' vast inflection; after 17' all but 15 inflected; after 2. all more or less inflected all but 8 tents * inflected or plainly only sub-inflected; after 4° 2' beginning to reexpand; after 7. 42', almost reexpanded

+ 16

Summary    Contrast the 8 first leaves, (yet slightly affected) poor leaves, for I had none good) & water not very hot, with the 8 under circumstances given above — I think I had better give detail in 2 parallel column. Of the 8, 12 — 11 all were affected in less than 25' 40; 5 3 were doubtfully affected; 5 plainly, but only one had almost all inflected & give proportion.—    Now of the 8 corresponding water spec. though most 1, 2, or 3 inflected for short time, one has 8, less than the doubtful ones in Phosphate & one alone had as many as 23 t. inflected — Yet we see from the poor leaves being hardly at all affected, & from only 1 out of the 8 phostes leaves on hot dry day affected, we have arrived at limit of action.

1874

(5) after 38', 28 inflected; after 3. 45' 33 inflected or sub-inflected — continued for 24° 3 days

(6) (11) after 6. 40' only a ring; after 9. 40' all but 25 t. inflected & lam: evidently inflected    after 24° 1 am. do & every tent except 1 inflected.; continued for 3 days

(7) 9 after 38' 42 inflected; after 3. 12', 66 inflected or sub-inflected; after 6. 40' all but 24 inflected or plainly sub-inflected, none very closely; after 9. 40' all but 17 do.    after 24º all but 4 inflected or sub-inflected & after 27. 40' lamina — continued

(8) 10 after 38', 21 inflected; after 3. 12', 46 inflected or sub.; after 6. 40' all but 17, none closely    after 24° every tent slightly sub-inflected; after 27. 40' lam. strongly inflected, continued.