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(p 74)

Sept 11th    8O 40'    1. 2.  Small balls of Caseine & 3 cube of cheese 1/20th of inch square: (Casein, Gelatine, Starch, Fat albumen nil) (Digestion)

(Sept 19th 10O 45'; the specimen in water is sill a non transparent sludge)

Th same in pure water .

(Sept 12' s 8O 20' tentacles upright, only a little affected; cheese fairly well embraced

   13    4O 40'    very slightly clasped. not so much as many inorganic substances cause—    Cheese well clasped & lamina inflected .

Sept 14'    10O 40' (1) consider inflection, caseine coloured pink (2) do, but angles of prominences of casein as sharp as ever (3) cheese, grt quantity of acid secretion, I think somewhat dissolved.) the spec of cheese in water grtly softened & all water turbid & discoloured.) (Sept 15    8O 25', both leaves greatly inflected, even laminae, but I doubt whether much absorption : cheese with leaf do & extraordinary quantity of very acid secretion & casein also causes strongly acid secretion.) (16th 8O same state; segregation, perhaps from inflection glands not blackened. I doubt any absorption : cheese seem rounded but very little absorption) : the caseine in pure water is all in a pulp) (Sept 17' 8O glands on disc whitened — same state) (Sept 19th 10O leaf now clear dead: the 2 with casein expanding but much left undissolved) (20' do)

11th.    8O 48'    4 & 5.    Gelatine soaked from 11O yesterday small square. ( & i squ in watch glass with pure water, no' 2) .

(12th    8O 30'    hardy any effect) (Sept 13th, 8O 40 gelatine liquefied, but has caused very little inflection— (The gelatine is certain as angular as ever. do Sept 14' 10O 30')

(Sept 14'    10O 40'    the gelatine on disc in same stat as on 12 & 13 see back)

   15    8O 40'    gelatine on disc as before, one leaf semi-inflected, but I think is dyng

(16th 8O 15' one leaf dead; the other leaf fully expanded with liquid gelatine on disc)18th 10O 30; same state)

(Sept 19' 10O 45' the gelatine in water still angular)    liquefies in 2 days    expanded in 4 days

11th    8O 55' Gluten from whet flower    No' 6 & 7' prepared by washing & in watch glass. Nor 6. (Sept 19th 10O 45 the gluten in water, still not opake sludge, no most light blue floating sludge) (12O    hardy any inflection) {Sept 13th, some inflection of tentacles of disc alone; gluten perhaps a little liquefied, certainly more than that in water.) (Sept 14' 10O 45, the gluten seems more dissolved transparent than that in water which is as opake as ever, but does not seem diss absorbed, very only slight inflection of closely surrounding tentacles; glands in contact much intensely blackened. (Sept 15' 8O 40' do, but 1 leaf considerably inflected; still much gluten on discs not dissolved but a rath small quantity was put on) (Sept 16th, the one leaf, red leaf, much inflected, glands intensy black, not very acid, no apparent seg: gluten very little absorbed: the other leaf expanding reexpand, a pale leaf, with gluten all over disc, very little or none dissolved— (see Back)    this leaf not at all inflected.

11'    9..    No' 8 & 9  Starch. of Commerce & the same in water watch glass 9. (12th    8O 30, great inflection, the lamina of 1 leaf inflected) Sept 13th 8O 45. much inflection, even lamina somewhat inflected, but as I can far see not rendered at all transparent.)

(Sept 14th 10O 45, nor 8 & 9 tentacles reexpanding not the least solution ,or is starch rendered transparent)

(Sept 15' 8O 45 leaves fully expanded — starch not in the least affected, leaves injured all smeared.)

17th    9. 3'    wit No' 10 & 11.    Cubes of an uncooked fat do in Watch glass 10. (12th    8O 30    no effect) (13th    8O 45' no effect, excpt tents. on disc a little bent toward other cubes.) (14th    10O 45', tentacles on disc quite straight not the least effect not of course in water) Thrown away

/over

135v

9. 5    2 cube of Albumen rather larger for comparison.    Stick with 2 pins .

( 12th    8O 30'    great inflection) (13th splendidly inflection & laminae— one cube which I cam see has angles all removed.—) (14th 10O 30' do.) (16th 8O 30 leaves I think dyng too large clumps) (18th    10O 30, 1 leaf dead the other dyng) X

Sept 12th.    8O 35' 12 & 13 put on 2 square of gelatine on 2 good reddish leaves, & the gelatine had been soaking since the 10th. — If this does not X cause inflection — try a solution, as I formerly experimented on the isinglass of commerce.—

Sept 13th    8O 30'    hardly any inflection

   14    10O 30', short tents. on the disc alone inflected, & there is much viscid matter on disc ,as if none had been absorbed, — Sept 15' 8O 20' in nearly same state  but 1 leaf is somewhat abut or semi-inflected.) 16 8O 30' very little inflection liquid still on disc, very acid no apparent seg. glands on disc of one leaf darkened. (Sept 17' one of the disc has caught little fly/)

   (18th 10O 30', 1 leaf fully expanded with some liquid yet on disc; the other which had caught fly oscillated over it, showing that it was good)

Sept 13th. 8O 30' 4 leaves    (matches with red Heads)    put on large dose of sol. of pure Gelatine    2 gr. to 1 oz . ( 16th but I find that nearly all the gelatine was not greatly dissolved)

Sept 14'    10O 30'    one of these leaves above very slightly inflected

― 15    8O . 15'    all fully expanded; now added good drops of Isinglass (2 dr to 1 oz) Sep 16' 8O 30' mere trace of inflection & liquid isinglass all done as if it had been water.)

Sept 17' 8O 25 — 3 of leaves fully expanded; one has caught minute fly & is beginning to contract

(18' 10O 30,    do    do    do, all the gelatine apparently absorbed; the one with minute fly inflected over its two ends showing leaf was good)

X

Sept 14th, 11O no 10 & 11 put a good biggish lump of gluten now become brown on 2 fine, new red leaves to see if inflection

Sept 15   8O 30'    little both leaves closely inflected also laminae .—

   16    8O 40'    do    do    do    do

   17'    8O 30'    one of the leaves killed brown & withered in an extraordinary manner & this shows how potent gluten is, for no vegetation matter wd thus kill

   18'    10O 30', the 2d leaf with all glands excessively blackened still closely inflected

Large pieces

X

Gluten

136

Gluten ( 6 & 7 ) continued — Sept 16 gluten in water, much softened & fine blue from some fungus ? (Sept 17th    8O 15'    in the open leaf with stained gluten looks healthy glands on disc a shade darkened & other inflected leaf glands very black, secretion still acid    (18'    19. 20'    The 2d leaf now expanding, glands blackened, much gluten left on disc : secretion still acid) (19th    10O 15 — Both leaves expanded do do do much gluten left on disc

p 74 3  

Gluten X

137

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(gelatin casein gluten (3 (74)

Sept 15'    8O 55' 3 Stick with red heads & blackened. good large square of pure gelatine which had been soaking in water since the 10th.

Sept 16th 8O 50' — 1 leaf with lam. much inflected, 2 slightly so : 3d very little only round disc)

― 17 8O 30' same state; secretion acid — one of largest pieces not yet all softened)

   18th 10 35'    very considerable inflection  in all 3 leaves: in one I cam see that all the squr is not yet liquefied : glands, somewhat dark on some of leaves.)

   19th    10O 35    the gelatine in each of all the leaves fully dissolved & liquid; one leaf beginning to reexpand

(20th ,2 leaves rexpanding — secretion very acid

(21st 9. — all 3 leaves reexpanding — glands a little darkened, great quant fluid ye on disc — I do not doubt from length of period of inflection some absorbed

   X

Gluten

Sept 15'    8O 58'    (8 & 9), very small bit of semi-putrid gluten gone putrid on 2 red leaves (16' 8O 45' L both leaves much inflected & lamina of one; not very acid secretion, perhaps all absorbed by gluten.)

(Sept 18'    10 23'    still closely inflected)

― 20    8' 40    1 leaf is beginning to rexpand, with a good del of gluten left on disc— In all case instances probably I put more than can be dissolved)

(21' 9. do) (Sept 23 8O 30' both leaves half opened— some gluten left on disc — glands of disc darkened.)

   X

Casein

Sept 16'    9. 10'    one sticks with black paper Heads. made 2 nearly perfect cubes of casein & put them on with a little water.

( Sept 17' 8O 30'    very little inflection, added a little water) secretion very acid)

― 18    10O 40'    Cubes still perfect, coloured pinkish)

(Sept 19th    10O 30    one of the cubes rather rounded) secretion very acid

― 20    8O 45    still closely inflected — intensely acid) (21', 9. do)

   23d    8O 30    a good deal absorbed & cube are now sphere

   24th    9. — 1 leaf with much secretion very very acid  other leaf becoming mouldy, also acid

   25    8O 15'    1 leaf reexpanding the other still closed— the single cube of casein surrounded by very acid viscid secretion — some of angles rounded, but margins very little reduced in size — very little absorption —)

138

(Pepsine Gelatine masses)

(4

Sept 16'    10O 42O'    — 4 sticks with red paper Heads

  "Pepsine porci" of a pig    wetted with distilled water & put on discs

   (11O 45' particles on glands do not act so promptly as meat

(Sept 17th    8O 40', 3 leaves rath closely inflected, one leaf only a little inflected : secretion extremely acid)

Sept 18'    10O 45'    3 leaves closely inflected & laminae of 3, the 4th with tents only on one side— . secretion very acid; so it is with the tents. bent at right angles & not near touching the pepsine.— — (19th  10O 20' leaves still inflected with a good deal of pepsine still on discs; secretion very acid) (20th 1 leaf beginning to expand

Sept 21 9. one reexpanding,— one closely inflected; one appears dead & mouldy

― 23' 8O 40' — 2 leaves mouldy, dead — 3d leaf with trace of pepsine on disc. reexpandng  I do not doubt that this leaf has absorbed a good deal : & the mouldy leaves much pepsine left)

X

Sept 16    11O 5    sol of pure gelatine    2 gr to 1 oz nearly correct — sticks with Blue paper heads    4 leaves .

   (4O 10' some slight inflection, not so much as below.

Sept 17'    5O 50'    some slight inflection in 3 leaves considerable in 4th leaf; secretion somewhat acid) (18th    10O 5' some consider inflection on 1 leaf hardy any in 3 others, added meat, roast) 18' 2O 30' all well clasped over meat, shows how much more potent this is) (Sept 19th    8O    laminae & all tents. immensely inflected)

X

139

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Sept 19th    10O 40'    3 Sticks with Blue Paper Heads

put some powdered Casein on & moistened with water to see if any residue : the stick with Blue & red cross had instead of water Hydrochlor A (1 to 874) — The Casein is excessively hard.

(Sept 20th    8O 50'    casein softened, the leaf to which I gave acid is much more inflected than the  2 others ' these latter have only their marginal discal & sub-marginals inflected.)

   (Sept 21' 9. well inflected), especially 2 of them.

   ― 23d    8O 45 — one leaf still closely inflected — a 2d rath closely a third reexpnding —    (thrown away)

Urea & Back

Sept 21'    8O 18' — 4 leaves 1, 2, 3, 4 with Urea large drop (1 gr to 1 oz) on each leaf

   (9. 15' no action) (10O ,30') (12O 35' ,O((4O, 0}

(22d    8O, no action or possibly a trace on 2 of leaves) Sept 23d 8O 30 I yesterday put meat on 3 of the leaves & thy are now most closely inflected; a 4th on which I left nothing is now sub-inflected & this made ne think action wd perhaps have taken place very slowly.    This evidently far less efficient than meat & so on

Back

Sept 22d    8O 18'    4. leaves immersed in sol. of Urea (Dr Moore)    1 gr to 1 oz    2O dr to the 4 leaves

(11O    some traces of inflection in one of leaves)

(2O 30', one leaf considerably, the 3 others slightly.

   5O    5O 2 leaves considerably & 2 very slightly

Sept 23'    8O 15' — all 4' leaves considerably inflected more than can be accounted for by mere water — washed & put into pure water

Sep

Sept 24'    9.    ,3 leaves almost fully expanded . one still closely inflected, changed water

(Sept 25' 8O    all 4 leaves perfectly in reexpanded) : this shows floating immersion in urea does have some effect)

Perhaps a little much matter

139v

Urea— (Dr Moore.)

Sept 23    8° 30'    put good large drops on 4 other leaves of sol of Urea 1 gr to 1 oz    (No 1, 2, 3, 4)

Sept 24'    9° 10' — no trace of inflection in all 4 leaves

(tomorrow add another drop)

Sept 25'    8°    no trace of inflection; added a large drop to each leaf (tomorrow add drop of juice of raw meat)

Sept 26th. 8° A.m no trace of inflection added large drop of infusion of raw meat —(at 11° 30' certainly some begin of inflection)    (2° 10 considerable inflection)

Sept 27'    7° 31' A . m    all 4 leaves closely inflected; we see how infinitely more powerful juice of neat is than the excretion Urea— as might have been expected

140

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Sept. 18th    Digestion of Fibrine  Red Sticks

8O. 30'    Red sticks with atoms of Fibrine, 2 rather large U 2 very small (NB particle put on glands cause immediate inflection like meat)

Casein Back

   2O 20'    well inflected over meat fibrin ie all more central tents

(19th    8O fibrin not all disappeared)

(20th 8O  2 of the leaves beginning to open with a little residue left ! so the fibrin not well digested or at least not all digested )

  — (These experiments show nothing except that Fibrin causes inflection, but is not thoroughly digested.    a residue being left)    ( Possibly too much given, yet I gave little)

8O 40'    do — Sticks with Pins    with a little Hydrochloric    1 to 437 of water (9.    fibrin looked gelatinous & swelled) (2O 30' added a little more acid of 1 to 874)

(Sept 19'    8O    In one leaf all the fibrin had disappeared)

― 20    8O    Three of the leaves pretty well reexpanded 2 almost fully reexpanded but some secretion left.    It wd seem that the Acid has hastened the digestion

8O 55; 4 Black sticks.    do    Black Sticks    do    with Carb. of Soda 1 to 437 of water.

   2O 30' added a little more of C. of Soda to most of then

(5O 15 added more soda very small drops)

   Sept 18'    8O 10' — leaves so closely inflected & so much blackened, I can make nothing of state of fibrine — Indeed this becomes so transparent in weak Hydrochloric & weak Soda, that it is not good for my object. (— Sept 20' 2 of the leaves almost reexpanded & in nearly same state as those above, but I then I fond out that 3 very acid, so was the 4th leaf which was still closely inflected)

(over)

140v

Casein

Sept 19'    8O 20'    put some casein with a little water on very pale leaves on old Plate to see if acid secretion for Frankland

(20th    8O 30    very acid on the disc but hardy any inflection ((Used) The secretion on the uninflected marginal (Used) tentacles not acid)

(21'    8O, 2 of the leaves have the sub-marginals tents inflected.)

   22d    8O    hardy any inflection but considerable acid in the casein & about pin

Sept 21'    at 10O 45' put some casein from Dr Moore moistened on 2 red leaves, at 4O P.m very acid (22d    8O    very acid both well clasped & lamina of one inflected; this greater action die to casein not being so hard) (24th    9.    do    intensy acid)

(Sept 25    closely inflected    intensy acid)

   28'    Both beginning to expand — intensely acid

(29     do    do    do)

(30th    do Hardly reexpanded    do)

(Oct 1' 8O A m — Th leaves still seem injured & are not healthy — the quantity of casein given was too much but still extremely acid secretion on disc— i.e. after eleven days

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(7

Chondrine — Haemoglobin

Sept 27th    10O 25 — Stick 6, 7, 8, 9 very moist gelatinous Chondrine (from Dr Moore) squarish pieces i.e angular — on 6 & 7 larger — on 8 & 9 smaller bits

Sept 28th    8O 30'    no 6 & 7 closely inflected & laminae glands rath blackened — all gelatine dissolved — very acid — no 8 & 9 small pieces — moderately inflected

(N. B the Dutch Chondrine put in water on 27th 8O for 24O 3 days keeps amply but swells round) (after 4 days disintegrated into little bits & nearly dissolved)

(29th,    8O    6 & 7 almost expanded — liquid on disc acid; 8 & 9 do. do)

(30th    all 4 leaves fully reexpanded  with a little liquid on disc only very slightly adhesive)    XX

――――——

Sept 30' ( 3O days)    8O 10;    (2) stick match with red Head ) chips of shaved chondrine 

Oct 1    8O 30' on one of these leaves on which there a broken slip of Chondrin had been placed is now quite liquefied & which are dissolved perfectly transparent— The other & much large leaf piece with the Ch much swollen & very soft — but not yet liquefied.

(Oct 2d    8O 55    the other almost liquefied) (Oct 3d, quit liquefied & almost all the whole transparent; it was rath large piece)

   X

   Haemoglobine (pure) Dr Moore Is not affected by immersion for 4 days in pure water

   Haemoglobulin    10O 30'    10, 11, 12, 13 sticks

Sept 27. On 10 & 12 added drops of water — not on others

   (on 12, the fragments rath small)

X  

   Sept 28'    8O 30' — No 10 very little inflection — moderate on the other three not more than often occur with inorganics

(Sept 29'    8O, 3 of leaves pretty strong inflected; in 2 of leaves glands beginning to dry)

― 30    8O    gland — content blackened & tentacles apparently injured— 3 of leaves closely inflected, the old & large leaf not so.) Oct 1', 8O 30' 10 reexpanding; no 11 & 13 dead : 14 look healthy rather inflected but I think reexpanding) (Oct 2d 9. the large old leaf now dying : no 12 reexpanding) (Oct 4th., do) (a) (Back)

Sept 28'    8O 30'    4 leaves 1, 2, 3. 4    with half minim drop of sol of Chondrin in Boiling Water 2 gr to 1 oz.

(12O in 3h 30' very strongly marked inflection in all 4 leaves; lamina of one inflected) (4th do)

(29th    8O A.m    only one closely inflected, 2 subinflected    Most of Chondrin absorbed)

(30th    8O    Three of 4 leaves fully reexpanded, (except 1 partially rexpanded) with all the liquefied Chondrine absorbed— — It agrees with animals— (see water or gelatine), that this substance is absorbed so quickly )

   XX

141v

480 (x) 2 (=) 960 (÷) 2 (=) 480

 

(a) Oct 4' 8O 30' The one remaining leaf had the few tentacles in contact with the particles stuck fast & mouldy — some gland beneath particles blackened — I do not think any of the particles dissolved— but softened— I may say is not acted on or only ? slightly— blacken some of glands & apparently somewhat injurious — — White dead patches a little while had been exposed a little oxide of iron, as if some decomposition had gone on —

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(9

Mucin Syntonin— Globulin.—

Oct 1'    8O 50    4 leaves 1, 2, 3, 4. (3 of these leaves had been acted on by chondrin but I thought were good) with dot particles of Mucin & Back moistened with water — before moistening were quite hard & dry —

(Also bits in pure water    Oct 2d    8O 50' unaffected

   Oct 2'    8O 50 — all 4 leaves unaffected, though a rath few tens on disc sub-inflected — Try them with meat ( & try hereafter 2 good leaves)

   Oct 3d    8O    all 4 .leaves unexpanded— put on bits of 1/2 roasted meat

(No' 3 (11O 20' no 3 with meat (I think this was a new leaf, a good deal of inflection) No 3 & 4, some inflect)

   (5th a good deal of inflection on all 4 leaves but not quit close; so rather feeble leaves, yet had few more inflected quicker than Mucin

Mucin Oct 3d which had been soaking in pure water for 2 days & was very soft put on 3 fresh & red leaves but rath old — Sticks Matches with red Heads — (3d one small leaf some little inflection)

(No 3 (Oct 4th. 8O 30' all 3 leaves with discals & very few sub-marginals inflected) (Oct 5 8O, do one leaf has very few sub-marginals inflected; discals still inflected) Oct 6th 9. same state) (Oct 7th 8O 45' secretion still very acid (1) leaf with discals alone inflected & glands dark (2d leaf with discals do, but glands not darkened. 3 leaf with discals reexpanded & gland very bright pink; the latter fragment removed, & I could perceive no trace of difference in angles on surface (Back)

Oct 3d.    8O 55'    3 Blue pairs sticks    Syntonin — put on some on dis gland & marignals, thy act became incurved in 3h

   (11O 10'    some inflection)

   (5O P.m—    2 closely inflected & lamina of one & a little with the other; the third leaf with all discals & some sub-marginals inflected)

X

( 8h)

Globulin    Oct 3    9. 15'    3 old red leaves sticks with whit paper Heads good-sized crystals on 2 & still particles on the 3' leaf & particles on exterior tents ( moistened the glob with pure water ) (The particle acted 2 or 3 minute on the exterior glands. of 2 leaves showing that these leaves are in good state) (Oct 4 8O 30— Gl in pure water with angles as sharp as ever.)

(11O 30'— the leaf with small particles much inflection)

(2O 40' great inflection of all 3 leaves)

(5O, all 3 leaves greatly inflected) : the piece in pure water apparently inflected)

Oct 4th 8O 20'— (1) closely inflected & lamina (2) almost closely inflect    I can see globules on centre surrounded by viscid fluid (3) little particles I can see some of them quit angular.)

(Oct 5th 8O 10', (1) not closely (2) pretty closely (3) with a few marginals & discals inflected.) (Oct 6' 9. no 2 reexpanding    particles apparently angles as sharp point as ever : glands drying on the 2 leaves as if gong to reexpand) But

142v

Mucin

from fragments which had lain in water since Oct 1 for this period long softened did not in any affected

Sept 9th 8O The 2 other leaves fully reexpanded, & in one the few discal glands which were in contact are a little darkened— Possibly a trace of some animal matter. — The mucin itself not at all affected ! — Th mucin which had been in plain water since Oct I much more affected for this from being so softened, that it cannot be lifted out—    yet, not disturbed keeps its shape—

Globulin    Oct 7th    9. 10'— Th leaf with particles fully reexpanded—    The other 2 leaves, with submarginals still inflected, glands dry, & becoming mouldy, but then many of the plants are growing old & failing — glands pink apparently have not absorbed — The angles of the particles on the one leaf as sharp & pointed as ever thy were & like those in water since Oct 3d, but even softer than the water specs

143

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(11

Sept 9th    8O 45'    Chlorophyll (blackened died too rapidly)    1, 2, 3, 5— 4 leaves — particles damp placed on discs

(10O,  40' some inflection on all sides of discals & submarginals)

(11O. 45'    excited much acid secretion, but it d(illeg) quick)

(5O, a great deal of inflection, the outer tentacles seem too old

Oct 9th    8O    very fairly well inflected ,(except no 3 & this seem too old & leaf still much very acid secretion —

   see back (overleaf blank)

Oct 11'    8O    on 1 & 2 laminae well inflected & tentacles; Nor 3 laminae & tent moderately inflected  no 5 tents well inflected

Oct 12'    8O 15 (1) reexpanding (2) closely inflected & lamina    (3) considerably inflected (5) closely inflected very acid —

Oct 13    9. 10' (1) & 2) almost fully reexpanded — acid — 3 & 5 reexpanding but still partially closed— There is very little appearance in the remnant of much or any dissolution of the chlorophyll — very unlike bits of meat or albumen —

144

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 8

   Pepsine & Swallows nest

Pepsine (pure from Dr Moore)    Oct 3d    11O 14' — Sticks 7, 8, 9, 10 4 new Red Leaves very good — put on so little pepsine made into points with water that I think wd certainly all be absorbed & disappear if it is digestible —

(2O 40' some inflection of disc tents & sub-marginals)

(5O considerable inflection all round discals & sub-marginals of all 4 leaves)

Oct 4th    8O 20'    moderately inflected  2 beginning to reexpand — some glands & disc colourless  showing that some effect produced

(Oct 5'    8O 15'    in no 6, 7, & 8 almost completely reexpand, nor 10 still partially inflected : & in all 4 leaves very peculiar appearance, discal glands, either central or a little on one side, from which fluid from pepsin has long rested very pale pink where the other glands round abut are very dark dingy purple — It is clear there no more absorption & that too much pepsine is injurious : the pale & darkened glands oscillated about in oddish irregular matter  manner : cut off no 9 & scraped disc which was covered with much very acid secretion & the fluid contained some of what I suppose to be minute rounded grains of pepsine) (Oct 6th 9. reexpanded — glands in the same state

   X

see Back

144v

(Pepsin    Oct 6th continued) it is clear from all analogy, that the addition of pepsine to the secretion quite is injurious & that the very pale glands prove this — the darker glands perhaps indicate that these have absorbed little that thy are not injured. — It is certain from quantity on the discs of all 4 leaves, seeing how little we put on, that very little indeed has been digested & absorbed — So seem to act like gastric juice & the dead stomach.— (see Brunton, where [illeg] prevented eating ??)

I compared under high power minute piece of Pepsin from the [illeg] & from the same pepsine soaked for 24O & thy presented exactly the same appearance in comparison —

X