38

July 31. 73    Cubes of Albumen 1/20th of an inch. — sheet of pure gelatine 1/20th broad an about 1/50th of inch long    (see Back)

A

(1) in (?) of pure water (6O 50' no change) Aug 1st 8O. 15' no chn

(2) in secretion ( describe how made) 10' 25'

   6O 50' gelatine rounded— (Aug 1' 8O 15. gel much rounded — Alb not affected but colored orange )

   Sol. not acid, I suppose albumen with alkali has neutralised it, added a minute drop of 2 per cent muriatic acid

(3)

(3) secretion    with C. of Soda in excess 10O 25' (do no acid reaction.)

   (edges brown of both brown) (6O 50' no change) (Aug 1 8O 15 do, but gel much dissolved)

   (Aug 1 8O 30' neutralised the soda & made sol just acid with 2 per cent muriatic acid

(4) secretion with a little weak muriatic acid of the Ph(illeg) I added by rod a small drop. 10 30'

   square of gelatine corroded into an oval—

   (6O 50' no change in albumen, gelatine greatly reduced) (Aug 1. 8O 20', albumen disintegrating round surface— gel much dissolved

(5) water with a little weak do. muriatic Acid    10O 30'

   2O 10' no square of gelatine — Did I forget to put it in — I have added another (6O 50' no change in albumen. 2d gelatine dissolved)

   Aug 1 8O 20 albumen not at all disintegrated

   Aug 2d    8O 10'    all the specimen in the same state

   3d —    no trace of action

   4th    do    do

      These experiments a complete failure

38v

Yesterday I put piece of glass on many leaves & today after about 22O I cut them off , but thy were very little inflected, & placed them in gr. of distilled water & after shaking them scraped off the slime.— I also then placed leaves in fine net & squeezed them. I got altogether about a gr. of very viscid discoloured fluid, which coloured Litmus paper. blue. & was used in equal quantities by eye & equal quantities of pure water for 2 of experiments in watch glasses, as on other side.

I then placed the watch glasses in Norway K with water raised to 86° Fah, & kept it there. About 110 leaves were treated as above.

(1)

Artificial Digestion

(By washing leaves too weak to act)

These experiments worth giving as showing that must not be weakened, & the negative results may by considered as Control experiments.

   Washing in water a complete failure

39

see Back

Kept at temp of room

(B

Aug 1. 8O 40'

(1) (12) pure water

(2) (illegible) . 9    secretion.    made slightly acid with minute drop of 2 per cent M. Acid (1O. 10' has become neutral added little more aid)

(3) 7.    secretion with C. of Soda simple C. of soda (shows no acid reaction)

(4) Secretion with minute quantity of Muriatic acid

(5)    water with a little weak muriatic acid

  Aug 2d    8O 10' specimen not affected

   Aug 3d    8    no trace of action

      4'    do.    do

The whole a failure

31

Abstracted

(24

July 22d    Carb. of Am.    1 gr to 200 oz ( 1/2 dr.)

(1) Water young pale leaf 8O. 31' ((10, 48' enormously inflected)) (transposition indicated with (...))

   4O 30' all extreme outer marginals closely inflected

Carb. young leaf 8O. 25' ((,10 50' tr ten or 12 marginals & shorter inner sub-marginals forming ring))

   (4O 30' only a ring on disc)

Aggregation see Back

(2) water young leaf 8O 32' (10O 50', outer discals a little inflected) (transposition indicated with '(10O ...' below)

   (4O 30' outer discals & inner sub-marginal forming a fine ring.)

Car. old leaf 8O 27' (10O 50' tr 2 or 3 marginals)

   (4O 30' trace of ring on disc) 

(I daresay intense Heat of day caused so much inflection in the Water specimens)

(3) water old leaf 8O 33' (10 52', s-marginal over pedicel inflect or sub-inflected)

   (4O 30' all reexpanded)

Carb. young leaf 8O. 28'    (10O. 53' 4 or 5 marginal inflect)

   (4O 30' ring on disc)

(4) water old 8O 35' (10O 53'   O) (4O 30', O)

Carb. 8O. 29' (10 '55'    2 marginal) (4O 30' ,O)

4O 31' Th water spec. more conspicuously inflected than the C. of Am spec

11O No effect from this dose of C. of Am

/over

39v

July 31.    I also took about 80 leaves with the glass & put them in 4 1/2 oz of distilled water & left them  there & agitated then for some time. This water did not coloured Litmus paper. I then decocted the fluid & strained the leaves in net & the fluid was still not acid.

I then placed the fluid in hot closet & evaporated it until about 1 dram  was left — Next morning I tried this & found it not acid!!