43

(F

Aug. 2d    Stick with lines.    4 glands with minute drop of acetate of Morphia (2' gr. to 1 oz) at 10O 39'  at 11O 1' put on meat on one gland (11O 9' not moved, 11O 20' do) (11O 35' do) (12O do) (12O, 30' meat moved) (ie after 2O. 29')

& saliva on 3 glands (11O two 9' moved a little) (1 not moved) (12O. do)

11O 2' put on saliva on 2 other gl. to right of pedicel & meat on other side —(11O 9' one with met meat well curved in, but not those with saliva at 11O 20') (at 12O those with saliva have moved)

Blue stick    10O 45.    Morphia to 3 glands.

at 11O 5' put on meat on one & infusion of raw meat on 2 others

(11O 10', no movement ; 11O 22' do) (11O 35' do) (12O, do) (12O 30', do) (2O 30' do)

(1O 10' the glands with infusion of raw meat a little inflected, & not 2 others yes I think thy did) (12O, do) (2O, 30, do. I think these tentacles with meat have been old torpid)

soon afterward also on tent. infusion of raw m & raw meat on other glands near pedicel —11O 11 all inflected.

Red stick, morphia to 3 glands for 20', put on bit of roast meat on one & saliva on 2 others, but leaf proved torpid as only a few other glands were affected by saliva & infusion of raw meat. — in 1O 33' one with saliva moved— in 2O  18' both the salivas & the one with meat had moved a little.

44

(G

Aug 2d.    look to Opium experiment in old Bournemouth notes —

As infusion of Raw & roast meat acts quickly, I cannot doubt that the glands must absorb.— (mem Opium) The most probable theory is that acetate of Morphia is an antidote & whilst actually on gland prevent action of organic or inorganic substance , but after a time the minute drop is all absorbed & then either act.

No doubt atoms of meat will act rather less quickly than saliva or infusions.

45

(H

Aug 3d.    8O 10' put in 4 leaves in A of Morphia (2 gr. to 1 oz) — & kept in for 15' Drained away fluid sol. & dusted with blue glass & grated meat at. 8O 30 (8O 45, O) (8O 54, O)

(X) (9. 15', O) (10O. 25' a shade of inflection in one leaf from the meat.

meat) (10O 56', in one leaf great inflection from glass, not in others, in Morph (10O. 59' in neither leaf any inflect from glass — perhaps a shade of inflect from meat;

(XX) great contrast with the 2 washed leaves below) (11O. 3', O) (11O 25', the meat has now decidedly curved some little inflection & I can see, tent with blue glass inflected) (11O 50' do) (12O, 30', same state) (1O 45', several tent some few tents. with blue glass inflected on both leaves) (2O. 30' same state)

The other 2 leaves wh. were washed well , i.e. shaken gently in water, but much slime adhered to them) drained away, & then dusted with glass & meat at 8O 32' (8O, 45', O) (8O 54', O)

(3) (9. 10', O) (9. 35' on both leaves a little inflection from meat

(10O 25 plenty of inflection from meat, but not from glass) (10O 57' in both leaves gr. inflection from meat, & in one from glass) (10O 59', on neither leaf any inflection from glass, a shade from meat speck (11O. 20' the tentacles with blue glass (4) in other leaf now inflected) (11O 50' do) (12O 30' enormous inflection in both leaves from glass & meat.

46

(I

Aug 3d    put in 2 d 4 good leaves in 2 dram of A. of Morphia (2 gr to 1 oz) & left in for abut 5', & then added 2 dr of Ph of Ammonia 1 gr to 20 oz

10' 43' Ph of Ammonia (10O 50 ,O) (10', 55', 0, (astonishing contrast between this saucer & that below) (11O . 20', O) (11O 50', O) (12O 30', O) (1O 45', O) (2O 30', O) (Aug 4th , 8O 15 the 4 leaves in the same state, but there is a fair degree of aggregation , not spherical ,not altered in colour, & extending down half or 2/3 of length of all tentacles, showing that there has been absorption; but nothing like the size of inflected tentacles below

Aug 3d.    put 4 leaves in 2 dr of same Morphia water & then added 2 dr of the same Ph of Ammonia

10O 43' added Ph. of Amm i.e. at same time as above , (10O 46' much contraction in two leaves, some in third) (10O 48' almost every tent in 2 leaves a vast no in the 3d leaf) (10O 55'    the 3d leaf with almost all inflected; the 2 first leaves with all inflected) (N.B the  sol. of Ph, is 1 gr to 40 oz, & 4 dr used) (11O 20 lamina of 1 leaf much inflected) The 4th leaf never became inflected, was a pale leaf—

Aug 4th. 8O 15' this latter leaf in same state & no segregation whereas immense in the other leaves, ie in the tentacles which were inflected.

47

(K

Aug — 3d — (Abstract & Conclusion from p. H & I)    It is demonstrated from p. H. & I. that the more of the A. of Morphia remain on leaf the slower it acts, & in whatever manner excited.

When the leaves were evidently well washed thy acted well . Ph. of Am. much quicker than when the Ph. of A. Acetate of M was allowed merely to drain away, a good deal being no doubt still retained by the slime from glands.— After a time all that is left is apparently absorbed & then whatever can excite the glands, does excite them; meat more energetically than blue-glass. —

But p. I shows wonderfully well, that as long as morphia, touches glands, even Ph of Ammonia causes no movement, — Does not Morphia act on nerves of animals? The former experiment shows that if little morphia is given it is pretty soon absorbed & power lost; but as long as on gland it stops meat & blue glass from acting. No doubt when I washed leaves &

/over