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Oct 4th /73/ I have been trying for change of colour leaves in C. of Ammonia 2 gr. to 1 oz. The glands are blackened in 2m; but even after 1h. 30m, I could see some purple in aggregated masses within the glands. I cannot quite decide whether the amm. does actually darken the protoplasm at first. — I can see after 1h. 30m that endless special spheres of protoplasm have been formerly as richly purple as ever, & only slightly darker from greater thickness & compactness. — After 1h. 30' most of the long-headed glands were rendered quite pale & from a short distance down the tents (∴ it is very improbable that the inky blackness of glands at first is due to any real darkening of protoplasm) & in a pale leaf, this discoloration had extended some little way down some few of the round-headed tentacles. (N.B in 20m after immersion purple matter much broken & aggregated & some few purple spheres formed.) (So that aggregation certainly not due to absorption.) (N.B Close beneath glands a few cells are greenish & at base)

(over)

[90v]

In 1h. 30m thousands of purple spheres had been formed.

[in margin:] no because not acid [bits washed]

Changes of colour fr C. of Ammonia — Aggregation & Redissolution

Oct 5th 8˚ Am, as many & as fine purple spheres as ever close up to glands — glands themselves more blackened — (N B It is not Carbonate but some salt of Amm which is absorbed, so that change of colour goes for nothing!!!) (5th (Put 8˚ Am into plain water after washing try a weaker sol. in the same manner; at 11˚ 15' (ie after 3˚ 15) I & Hooker saw spheres travelling with greatest plainness) so that immersion for 24˚ in the sol. of 2 gr to 1 oz had not killed leaf— some dissolution of protoplasm had commenced; at 4˚ 45' a good many spheres had become elongated.

Oct 6th 8˚. there were still a multitude of purple spheres, but here & there a cell was filled with fine purple fluid, showing that a good deal of dissolution was had been effected. A pale leaf, however, which had been at same time similarly treated seemed killed; at least I saw no signs of dissolution of protoplasm. Redissolution