(1

Eastbourne

Drosera rotundifolia

Oct 3d. at 10° 20' put 2 leaves in Urine & one in water

at 12° Both those in Urine had colour in all hairs wonderfully broken up — that in water no change. — Those in Urine with hairs somewhat inflected & long marginals a good deal curled curled, but not properly inflected as in growing growing leaf over fly. Leaves decidedly flaccid, far more than that in water

At 2°. 20' (ie 4 hours) hardly any effect on leaf in water— vast action in that in Urine

At. 4°. 35' (ie 6°. 15' hours) On sub-marginal in water spec. with broken end, & three inflected but leaf not flaccid; long- & round-headed marginal with end not broken.—

(Water)

[1v]

(These leaves were very pale coloured young leaves)

(2

Oct 4th 7' before 10° A.m. Carb. of Amm. 3 gr to 1 oz

10°. 4' (ie in 11') lots of spheres formed — process of formation running runs down hairs saw this in every innumerable cases. I saw one larger sphere attract & suck in a small sphere — In 11 minutes action had proceeded down thrice length of Head (not a long-headed, but marginal round-shaped hair)

At first in homogenous fluid little spheres appear, only just under 1/4 object glass & those rapidly increase in size; & the action proceeds down the Hair—

in 2 1/2 hours action had proceeded fr 1/2 to 1/3 down each hair. At 4° (ie 4 hours) every hair down to roots had broken matter, probably took place much earlier

In 35 minutes no action in sugar of same strength as the C. of Ammonia (viz 3 gr to 1 oz) (except on that side on which there had been accident & some spilled.)

11'. 45' These 2 specimens (ie that in C. of Am. & that in the weak syrup) in same relative states.

11°. 50' (ie after 2 hours) put C. of Am. solution on the leaf which had been exposed to sugar— spec.

at 12° conspicuous change!!! Clear case.

3)

Oct 4th Dark red leaves of D.

Barely 20' minutes to before 11°; in 3' close to the head shrinking plain from Carb. of Ammonia 3 gr. to 1 oz & incipient division into small spheres; in 5' process had proceeded more than length of Head. The hair watched was a marginal round-headed. This was dark red specimen— The change was different from last which was very light-red; in here this having is more red fluid in the cells, matter & instead of innumerable small spheres first appearing, & then a shrinking & then division into a few large masses or several smaller large oblong & spherical masses were formed & contents of cells shrink; & almost contemporaneous large masses divided minute spheres crowded together — there is change of colour from the C. of Ammonia, which changes red in brownish green; this change chiefly in upper part of curled Hair. It is quite evident long- & short-headed glands are absorbent as well as secretor like roots. The process is arrested at each cell-wall, & then when breaking up commences whole contents of each cell is broken up almost [illeg] — It is only upper part of hair close to gland which changes colour

In 24' change had proceeded more than 1/2 way down hair. In middle of hair only small spheres as in last specn. Change takes place slower in lower half of Hair. Long & round-headed equally affected in some same manner.

(4

D. rotundifolia

Oct 4. 11˚ 28' 3 gr of sugar to 1 oz of distilled water dark red leaf put in solution of

11˚ 44; no change

12. 3 no change

12 33' no change or if any most slight

1. 20 no decided change if any. —

1. 55' hardly any change.

2. 18 do

2. 45 do

3. 44 no change

4˚ no change (ie 4 1/2 hours)

At 4˚ 3' 30' took away weak syrup & added the weak C. of Am. 3 gr to 1 oz, carbonate of Ammonia; in 5' the length of 1/2 head with spheres; in two hairs all broken up into spheres: in 10 minutes length of long-headed Head broken up.

Head of Long-head becomes green. —

In spec tried this moving change has worked down to very base

[4v]

Long-head & round-headed Heads have the groups of spiral cells —within the Heads. Roots pink at tip, but no structure like in Hairs —

Long-headed secrete only on upper surface when gland exposed.

(5

Oct. 5th Nitric Acid. 1 min to 1 oz.

9˚ 47' dark red Hair exposed for nearly 1/2 hour; I am not sure any effect.—

10˚ 30' another spec. in nitric acid 10˚. 55'. 25' no marked change.

11˚ 29' no change, at least marked, but base of all heads had colour somewhat broken

at 11˚ 29' put in C. of ammonia 3 gr to 1 oz. 11˚ 40' whole cushion of long-head turned dull purple; spheres increasing at its foot & in all cells at back of cushion.

11˚. 58' no marked effect 12˚. 47' [-] 11 29 [=] 1˚ 18 no effect

(C. of soda Soda 2 gr to 1 oz 10. 35 (pale leaf)

10. 55 no marked change —

11˚. 35' no marked change

12˚ 47' no marked change 11˚. 40' put in C. of ammonia in 4' length of head of round-headed broken up into cells. Heads all green.)

12. 50 put fresh C. of ammonia. — Heads turned green I see.

This new dose in 5' minutes produced strongest effect. So nitric acid greatly retards action of ammonia, b it seems to neutralise it. How does N. of ammonia act. —?

(6

Oct 5. Put leaf in thin Gum (far thicker than the 3 oz of sugar to oz) at 1˚. 8'

no change or in colour by 2˚. 50' (=)

at 2. 53 [-] 1. 8 [-] 1. 45 put into solution of C. of amm.

3 gr to 1 oz.— in 5'. length of head all broken into spheres. —

Oct 5 put leaf in White of Egg which had been kept 2 days at 3˚: at 4. 25. (ie 1˚. 25' say 1 1/2 hour) no change or in colour

at 4˚ 27' put in C. of am. 3 gr to 1 oz in 5' conspicuous spheres from length of head of round-headed

I believe cells are left full of clear fluid it is the rapid aggregation of matter dissolved in fluid of cell. — it is curious to watch how the cells spheres swell & absorb each other.

I may be sure that cells are full of fluid from the cases of circulation of particles

(7

Eastbourne

Oct 6. — Leaf in Milk at 9˚ 27' in one hour the red masses were just decidedly a little more broken up, & a little more separate than before; only just perceptibly.

Oct 6. good red Leaf in fresh Urine Urine at 9˚. 30' in one hour: at 2˚. 15' — one hair hardly at all affected; perhaps trace of effect— the other slightly broken up. — At 5˚ (ie 7 1/2 hours) no decided effect; then added C. of am. (3 gr to 1 oz) & it took 15 minutes for decided effect in causing spheres to form; could this fresh, morning Urine have had much Uric acid? This same Urine kept for 53 hours, produced no effect in 1/2 hour. Nor was there any effect in 1˚. 30'.

Wine

Oct 6' Sherry wine. Leaf put in spoon-full at 11˚ 14; at 11˚ 28' I imagined a decided effect, but at 12˚ 50' (ie 1˚. 35') there was no sign of breaking up, but from endosmose, the signs were red was all uniform & unbroken in all the hairs Ø

The leaf was rendered nearly flaccid

[7v]

Oct 7th took new leaf which had naturally caught fly & had broken red & put in glass of sherry sherry — some of Hairs opened naturally & all long but could be turned back quite easily — Colour all became quite uniform.— left in for 9˚. 10' to 11˚. 18' ie 2 hours.

(8

Oct. 6th Carbonate of Ammonia (Eastbourne) Segregation

Whole Leaf put into weak solution.

(1˚) 1 gr to 1 oz distilled water

put in 10˚ 28'

― 32' (4') decided change, (ie always means formation of spheres)

― 37 (9') change extends length of round-head; & colour changed — observed in all Hairs

(Used for rate) C whole L

(2d) 1 gr to 4 oz water (N.B as I took 1/4 of Tea-spoon in each experiment, the solutions are really weaker)

put in 10˚ 41 say 20-30 m

10— 45' (4') change 3 spheres arose

10 47 (6') change in all hairs for length of round head

C whole L

(3) 1 gr to 7 oz

put in 10˚. 50'

― 55' little effect

11. 1 (11') decided change, in structure & colour in all hairs for nearly length of Head— N.B the spheres formed are very minute

C whole L

[8v]

It is possible I may have blundered in adding to water, but I think not.—

[calculations not transcribed]

Put the remainder of leaf in glass of solution at 9˚ 43' — (too much torn to tell anything)

Put another fine red leaf in same glass of solution at 9˚ 50' 10˚ 18' all heads blackened & a few spheres formed everywhere; but I did not previously examine spec. ß

(9

Oct 6th Carb. of Ammonia Eastbourne

Segregation

1 gr. to 10 oz = (1 to 437.5 0 by weight)

put in 11˚. 7'

11˚. 12' (3') colour changed perhaps trace of breaking up into spheres.

C

Oct 7th Made solution of C. of Ammonia 1 gr to 12. oz. took pale red leaf; cut off 1 hair & put 9˚. 40' under slip, with 1/2 minim of pure water, (water) (a) Back 10˚ 26' no change ie in 46'

Cut off two hairs & put with 1/2 m. of the solution & put under slip. at 9˚. 42 —.

at 10˚ at 10˚ spheres increased slightly in number & size

at 10˚ 7' (25') severl spheres 10 or 12. there were originally 3.

10˚ 13' spheres decidedly larger

10˚. 27' (ie 45') spheres have certainly increased in size & now 10 or 12.

Heads not blackened or greened. (No use trying weaker)

[9v]

(a) It is most singular that weight of glass did not cause movement segregation can water check the process? Is cause wing of glass

(10

C. single Hair Segregation

Oct 7 same experiment as last with new leaf C. of ammonia 1 gr. to 12 oz, 1.2 minim taken

at 10˚. 38' put into the solution under slip of glass

10. 58 no decided effect

11. 26 no decided effect, except I think in Head, in which red masses now spherical

11˚. 35' spheres have now appeared under Head. (in 57') ((a) Back)

11. 48 (in 70') a dozen spheres & red-head— spheres larger; uppermost part of stalk part green.

12˚. 30' — (in nearly 2 hours) Breaking up has now run down to pink about length of head.

C Whole leaf

at 10˚. 38' put whole leaf into wine glass. with the solution.

11˚ 18' Heads blackened.

11 35. I have no doubt effect produced just below Heads. greened & rather spherical.

35 [-] 18 [=] 17

Water C. Single Hair

at 10˚. 34' put a hair into 1/2 minim of pure water under slip— no effect. exc

11. 53 except perhaps a few spheres in water but previously laterally to tip but No spheres in red heads. 12. 30

(Appearance of this utterly different from that of Ammonia now ie 12˚. 30' — (Head not so dark a purple.)—

[10v]

(a) I am not absolutely certain whether this not due to incipient drying up.

It is impossible to doubt great change; whereas certainly no such change took place in 1˚. 16' in the 3 Hairs in plain water. ß

at 10˚. 22' (when I left off examination) red breaking up, spheres increasing & absorbing each other. —

(11

Segregation Water

Oct 8' cut off 3 Hairs (of fresh arrived spec) fine red & put under plain water at 9˚— 9 at 9˚. 47 no change

at 10. 16 no change (ie 1˚. 16')

Eastbourne

put single Hair C in sol. of Carb of ammonia 1 gr. to 10 oz; i.e the gland received 1/9600 of gr of C. of Am. put in at

at 9˚. 13' when put in trace on one or two minute obscure spheres under head

at 9. 43 (ie 30') fine spheres were conspicuous & one part of red was broken up in egg-shaped mass & the red of the head certainly more divided or reticular

10. 13 (ie 1˚)

9. 47

at 10. 13 (ie 1˚) there were 8 large spheres & several minute ones some way up stem: the red oval had become spherical & other part of red more broken up. Can even see spheres with 2/3 object glass & weak eye-piece. Head tesselated appearance.

C. Whole L

Put whole leaf fine red in tin Box in sol.. of 1 gr to 12 oz, put in at 9˚. 20'. The quantity of fluid afterwards measured was 5 minims say 6 to allow for evaporation. — at 10˚ in every spec. some spheres under Heads — Heads, I think, look browner. —

10˚ 21 (ie 1˚) 4 to 6 spheres can now be seen Ø

[11v]

with 2/3 object & weak eye piece in every hair. At 1˚. 30' I do not think spheres increased in number. At. 2˚. 30, perhaps spheres decreased in number.—

The red in several Hairs in lower part is now broken up, but I did not notice this sufficiently.

80 hairs round borders, & at least 20, (certainly more) on disc. say 100 & all apparently affected. —

60 36

480 minims in oz

[calculations not transcribed]

According to this calculation each head could have got only 1/96,000 of a grain of carb. of ammonia. —

(12

Oct. 9th — Cold day Segregation

Excellent fresh pale leaf carefully examined in water with 2/3 O.G. & weak eye p (Whole leaf) C. pink in stems uniform no spheres at base of Head— Heads maroon & not broken. — Put in Sol. of Carb of ammonia. 1 gr to 12 oz

at 9˚. 30 (error)

at 10. 35. Emma & self thought Heads redder & more transparent— internal structure more visible— base of head greener. In one or two hairs alone a few spheres (With stronger eye-piece I can see cells in many) 11˚ spheres plainer 11˚ 35' spheres not plain

12˚. 30' no change 2˚ no more effect — 2˚. 5' put with fresh solution of 1 gr to 12 oz see Back

at 9˚ 37' carefully examined another exactly similar leaf in same state & put in plain water (error)

10˚. 45' I can see quite as many cells in this on specimen; & I think Heads quite as clear!!! & green is plain at base!!

11. 15 spheres plain, quite as plain as in amm: spec

12. 30 in same state as ammonia spec 2˚ same state

at 2˚ 5' (ie. after 4 1/2 hours) put this leaf into C. of amm. 1 gr to 12 oz

see Back

[12v]

at 2˚ 5' I doubted whether I really had put any sol. of C. of ammonia to this spec. so made fresh solution & put; at 2˚ 55' all the Heads are black & spheres more apparent; 3. 30 all the Heads jet black; even the long-headed, which were paler very pale dirty purple are now nearly black— Many of Hairs now have conspicuous spheres, 1˚. 35'

4˚. 5' Heads absolutely opake— Transparent borders of long-heads crowded with cells. Spheres larger & more numerous in all Hairs

Pink no where broken,—

5˚. 5 quite large spheres in almost all round heads, extending down for 1/2 length of head to almost Heads' length

In long-headed, elongated masses or ovals instead of spheres encroaching on what was pink

at 2˚. 5' (error) I also put the Hairs which had been in Water into the now weak carbonate (the same ie 1 gr to 12 oz) to the specimen which has been in water & this also 3˚ Heads conspicuously blackened.

3. 55. These Heads of both kinds now quite black; in some Hairs spheres far more conspicuous.—

4˚. 10'. long-heads bordered with crowded cells—

2˚. 5' Some Hairs (most in nearly same state) near stalk with numerous spheres. — Heads quite opake & black.

5˚. 15 The spheres in most of hairs less developed than in other spec. but in some quite as much & this leaf seem lately to have caught fly; pink was on this side spheres not so much developed.

[13]

Segregation

I think C. of ammonia mostly act as a ferment & so transmit transmitted the process of change. 6° 10' The spheres are pink.—

I see in long-heads the red is broken up— The process has travelled some way down hairs; in some twice length of Head.

7° 30' Breaking up of red (as seen with stronger eye-piece) running very far down some of Hairs.

10° 10' P.m (I observe hairs on disc similarly affected as those near margin)— the breaking up of red has run down far in some Hairs Error

6° 15 Hairs which have changed are changed as much as above specimen: 7° 30' in nearly same state as above spec. — 10° 15 same state red breaking up down footstalk.— Error

It is impossible to doubt next day I made same blunder & perceived it with Acetate of ammonia that my suspicion was right that I put no C. of ammonia the first time: I might easily have forgotten, for I know I added water first to glass—

This only doubt in whether where leaf kept

[13v]

in pure water for above 4 or 5 hours, whether the heads blacken; if not the case in these two specimens clear.

Error

Wednesday 10th 9˚ In many Hairs segregation of pink stuff had run down 1/4 or 1/3 length of Hairs — Same with other specimen.—

10th 12˚ Keeps as black as ever in Heads breaking up has proceed far down long-headed Hairs

Each Head 11/2000 of an inch in diameter

The Heads on disc felt as large as those on margin — nearly all equal.— As Heads all oval; taking spheres will allow for allotment of footstalk — Heads about 15 or 16 / 2000 long

(Add to [illeg]) ä

[calculations not transcribed]

(14

Oct 10th 10˚ 21'— Fine fresh leaf first examined in plain water with 2/3 & strong eye-piece & then put into Acetate of Ammonia about 3 gr to 1 oz. (a very few spheres at base very minute — back of long heads with no plain spheres— colour of Heads maroon red bright)

at 10˚. 21'

10. 31. (ie 10') many Heads blackened; I believe clear signs of breaking up some way down Hairs— Long-Heads pale purple

10˚ 36' (ie 15') breaking up clear has proceeded down length of Head — no cells yet back of long-heads— these not so dark except one or two

10˚. 55' (24') the breaking up of red matter has now proceeded far down stalks — not so many spheres formed as with carbonate of Amm. more contraction in red matter some way down stems — Heads I suspect not so much blackened.

The action seems to commence at base of spheres—

I see very little spheres oscillating about, as I have often seen before

11˚. 15' spheres forming back well formed on back of some long heads (in 54')

11. 35 all long heads now have spheres back 1˚ 11'

colour not so dark of Heads— The breaking up very distinct but does not proceed — I think any further

[14v]

Oct 10th 3˚ 21' new Red Leaf in Acetate of Ammon Ammonia about 3 gr to 1 oz. — No cells blackened long-head— only two of breaking of red matter in some round-heads.

in 19' an undoubted great effect a long way down the hairs in breaking up of red matter & formation of many spheres— Heads darkened. Trace of cells forming at back of long-Heads. —

4˚ — Abundance of perfect spheres in common Hairs & some spheres in back of some long-heads. (39')

5˚ Tremendously broken up — spheres in back of all long-heads. —

1˚ 40'

5˚. 45' I think spheres have ceased to increase perhaps in long round-heads have decreased — Yet with high power spheres splendid

9˚ 40' the spheres. extent far down almost all Hairs

11th 9˚. 15 Hairs nearly all mostly colourless; with minute spheres down in most to base

(15

Segregation Water

10th. 12˚ leaf in pure Water at terminal end some Heads dark. In very few Hairs faintest trace of breaking up & very few most minute spheres. — no spheres back of long-heads

2/3 & strong eye piece— Every Hair examined. — no change 2˚. 15' in any respect.

5˚. 45'. no change in either Heads

9˚ 40; (ie 9˚ 40') absolutely no change

11' 9˚. 20' Heads — Astonishing number of minute crystals on glass. (9 minims.) See p 16 for these spec. & am

8 minims in fine Box

10th. 12˚. 48' — old red leaf. Heads nearly black.— in long-headed Hairs much broken near Heads—

In round-headed trace of do, but not transparent.

Solution of 3 gr to 1 oz of Nitrate of ammonia

2/3 strong Eye-piece.—

1˚. 40' (52') In round-Headed, the red masses more separated ends more rounded with daylight seem between; & some large spheres formed— on left side back of long-headed without spheres. 2˚. 10' (ie 30') Plenty of true spheres formed p. 22 in almost all round-headed— In most of long-headed either spheres formed or forming in back part

3˚ immense spheres now  2˚ 12 just like in an ammonia specimen— Lots of spheres behind Long-headed-Hairs

(16

Segregation whole leaf

Oct 11th. — Leaf which had been in water unaltered since 12. (ie nearly 21˚ 15' hours) was put into fresh solution of Carb. of Amm. 1 gr to 12 oz of water Strong Eye-Piece

at 9˚ 49' C.

at 10. 49 to 55'. Heads all black — in many Hairs splendid red spheres & ovals— Heaps of cells at back of all long-Heads.

11˚. 49' to 59' every single Hair with some spheres; in some few nearly down length of Head— generally only half head— many cells in back of every long-head. all Heads, including long-Heads pitchy Black. (B) (B)

(Finished)

See leaf A for Oct 11 — Fresh large red leaf examined in plain water Strong Eye-Piece

C. Whole L. C. (a) & then put into Sol. of Carb. of ammonia of 1 gr to 16 oz of water: no breaking up no cells behind long-Heads — colour of some dark red— in some so dark only trace of red cd be seen.

at. 10˚ 5'

11˚ Except in one long-Head no change— (Long-heads now dirty pale purple)

11˚ 35' traces of breaking up in some 6-9 round. Hairs no cells behind long heads

12˚. 20' in most of long-Heads some breaking up below Head.

1˚. 45' not much change. (in 3˚ 40' not much change)

3˚ 5 Hours. I can now with care see very minute spheres at base of almost all long-heads. — Heads, I think, darker. — (over)

[16v]

(B) The fluid carefully measured was 6 minims say 7 for loss.— 146 Heads carefully counted. — see supplement page for grains

Oct 12' 9˚ 15. This leaf after 23 hours had all Heads, especially long-Heads blackened: in one or two Long Heads few cells behind Heads

In the Long-Heads, the parts beneath Heads minutely [illeg].

In the long & moderately long round-heads di pink matter segregated in all— & in lost of short I could see some trace of effect — From repeated trials with same leaf I fell sure that never above 5 minims of solution was in: for I flattened compressor down on this leaf 189 Hairs of which 81 long & all effected plainly; 108 short, but these examined with high power all showed signs of change in few spheres beneath Head & I believe darker coloured

(a) This spec. had very few long-heads; & I saw one in exactly intermediate state [sketch] between round-Headed & long-Headed.—

5˚. 15' P.m. continued — spheres not larger anywhere. I have no hesitation in asserting that pink more broken up do for full length of Heads down longer hairs, that than it was when first put in.

9˚. P.m. There can be no doubt about breaking up some way down of red matter in many leaves — no cells behind long-heads — no great change of colour in Heads X 1. to 16. oz.

16 Bis

[calculations not transcribed]

16 Bisv

[calculations not transcribed]