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April 2d. 1874 8° Am. a bit of bone from boiled roast mutton chop in sharp rough splinters with little points.—

No. 2. — also 2 bits of smooth Hyoidal bones from fowl— not cooked (& kept dry for some months) — all 3 moistened with saliva.

(5th all 3 leaves (laminæ) splendidly closed over bones)

April 11. 8° 30' First for bit of mutton-chop Bone— Boiled — Tentacles beginning to reexpand — much secretion very acid.— This bone was so hard that it requires my whole force to cut off a fragment with sharp wire pincers— now softened & transparent — so that with 2 needles can be separated into splinters & flattened by pressure —The

The pointed fragments seem to me still to contain some bony matter in middle, surrounded with cartilage, which apparently has not been digested

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(Bones) 1874— Hyoidal Bones

Ap. 11' 9°. secretion on both leaves with these bones ar very acid. —

One leaf still embracing bone & lamina still inflected!!

(The other leaf almost fully expanded bone removed & examined under simple microscope— The colour is surrounded by very tough transparent cartilage in centre there is apparently a column of little altered bone, I judge from appearance & having cut off a bit at end, I found a small portion which was gritty under needle, whilst all the rest was tough & could be [torn] without any grating noise, Dr. Klein has been so kind as to examine & give me the following description of the state of the Bone (see Frank's M.S)

Ap. 12th. 9°. The 2d bit of Hyoidal bones now unclasped — so that clasped for 10 days!! I never saw so long— Is it animal matter in vessels & within bones corpuscles of is it the Phosphate?!

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April' 12th 1874 (Bone)

11°. A.m. put the bit of same Hyoidal bone, which had been clasped for 10 days on fresh leaf, with a little saliva — to see if the fibrous tissue can be dissolved.

Also at 11°. 15' the (2 now separated) bits of rib of sheep, which had been clasped for 9 days — In both these specimens all the Phosphate of Lime removed.

Ap. 13' 8' A.m. Laminæ & tent. of both leaves clasped over both bits of Bone.

Ap. 18 ie after 6 days The leaf with Hyoidal partly open. Ap. 19 8° A.m. quite open.— Ap. 19' after 7 days 8° Am. leaf with sheep's bone almost open. — so this latter closed for 7 days — not so long as before, & I suppose cd not get so much Phosphate. On both bones there were ragged sharp points at the ends & these are now in exactly same state as when put on. It is certain that D. can not digest the fibrous tissue of bone!

Can man or dogs? if not triturated.— The secretion on both leaves (Ap. 19) very acid, so by long inflection & acidity, certainly active

As far as I can judge minute structure the same as when Klein saw it. —