8

April 12 — 1874 experiments by placing leaves in warm water & not using C. of Ammonia

Temp.
150°	2 tin (1 lead) put in	8O 9 57		(1)
145 145°	Pin	8O. 9 58'		(2)
140°	(2 lead)	8O 9 59	(soon become a little had become little inflected on both sides from being touched	3
130°	2 tins put in by mistake at 130° & raised to 150°	8O 9 55	& left in take water to 150°	(4)
125	1 tin	a little inflected left hand	9. 2'		5
120	O mark	9. 4'			—(6)

   New leaf

Fresh. experiment

Temp. 130° water. waved for 1m.    certainly caused aggregation   
Looked at under compound [microscope] effect as soon as I could    effect oscilation arrange micrometer, in 2m or 3m

Temp 125° fell to 119 waved about for 2m, great inflection in. 2m to 3m or 4m when looked red protoplasm had shrunk a little from walls of all the cells, down to base of tentacles; & some elongated oval masses aggregate protoplasm shrank from cell walls all tents. finely inflected — aggregation down to base — cannot be exosmose —       This cd hardy be exosmose so quickly.—
   5.O p.m tentacles in nearly same state; so spherical masses are not formed 10O P.m.   I thought protoplasm somewhat dissolved
April— 14th 7O 8 45 In some of tentacles I am nearly sure entirely dissolved but not in others
   (add few spheres for warmth)    (Ap 15th nil 8O A m Greatly Redissolved)    3O Pm much smaller & 2 have disappeared

diagram

1O
7O 8 45'
??
no

8v

(6.)    10O 30' very little inflection & no agg.
Tem 120°.    waved for 1m. Single M. .— (11O no aggregation to speak of after 1h. 26m except a trace in a few inflected tentacles & here only here only a trace

(1)    10O 40'    glands all white no aggregation    perhaps finely granular.    150° Glands white    after 1h 43m

(2)    10O 41    glands all white (145° nil Fahr)
140°    no aggregation.    after 1h 42m 43


31    10O 44'    glands all white (140° Fahrn.)
   a good deal of inflection no aggregation
      after 1h. 44m    leaves left in water to cool    Hot House plant


(4)    glands all white    put in at 130 & raise to 150
   some inflection    no aggregation—

after 1h 46m.

(5) 10O 55'— to 11O — ie 2 Hours a great deal of inflection.    all tents very bright red
(pink)    certainly some aggregation —(12O strongly marked aggr. but not spherical)

Temp. 125° after '3h.' below After 1h. 48m.       after 3h.

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