8

April 12 — 1874 experiments by placing leaves in warm water & not using C. of Ammonia

Temp.
150º	2 tin (1 lead) put in	8. 9 57		(1)
145 145º	Pin	8º. 9 58'		(2)
140º	(2 lead)	8. 9 59	(soon become a little had become little inflected on both sides from being touched	3
130º	2 tins put in by mistake at 130º & raised to 150º	8. 9 55	& left in take water to 150º	(4)
125	1 tin	a little inflected left hand	9. 2'		5
120	O mark	9. 4'			—(6)

   New leaf

Fresh. experiment

Temp. 130º water. waved for 1m.    certainly caused aggregation   
Looked at under compound [microscope] effect as soon as I could    effect oscilation arrange micrometer, in 2m or 3m

Temp 125º fell to 119 waved about for 2m, great inflection in. 2m to 3m or 4m when looked red protoplasm had shrunk a little from walls of all the cells, down to base of tentacles; & some elongated oval masses aggregate protoplasm shrank from cell walls all tents. finely inflected — aggregation down to base — cannot be exosmose —       This cd hardy be exosmose so quickly.—
   5.º p.m tentacles in nearly same state; so spherical masses are not formed 10. P.m.   I thought protoplasm somewhat dissolved
April— 14th 7. 8 45 In some of tentacles I am nearly sure entirely dissolved but not in others
   (add few spheres for warmth)    (Ap 15th 8. A m Greatly Redissolved)    3. Pm much smaller & 2 have disappeared

diagram

1.
7. 8 45'
??
no

8v

(6.)    10. 30' very little inflection & no agg.
Tem 120º.    waved for 1m. Single M. .— (11. no aggregation to speak of after 1h. 26m except a trace in a few inflected tentacles & here only here only a trace

(1)    10. 40'    glands all white no aggregation    perhaps finely granular.    150º Glands white    after 1h 43m

(2)    10. 41    glands all white (145º Fahr)
140º    no aggregation.    after 1h 42m 43


31    10. 44'    glands all white (140º Fahrn.)
   a good deal of inflection no aggregation
      after 1h. 44m    leaves left in water to cool    Hot House plant


(4)    glands all white    put in at 130 & raise to 150
   some inflection    no aggregation—

after 1h 46m.

(5) 10. 55'— to 11. — ie 2 Hours a great deal of inflection.    all tents very bright red
(pink)    certainly some aggregation —(12. strongly marked aggr. but not spherical)

Temp. 125º after '3h.' below After 1h. 48m.       after 3h.

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