(8

June 4th

Pinguicula

The secreting glands are seated on a unicellular colourless footstalk, the gland or head is divided int about 16 divisions, & is broad with ova circular as seen from above — oval in [sketch] — in fine light green fluid homogenious—

Besides these are endless much shorter glands on short footstalks divided into generally only 8 divisions & of a much paler green; these I believe were only absorbent, no no & will utilise the flowing secretion

Put in C. of ammonia 2 gr to 1 oz, the contents of the tall & of many of the shorter glands become brown & granular — & more opake & in pedicels the content shrink from walls. — (I believe all glands secrete) — I think shorter glands absorb C. of am. before the larger ones.

I put drop of same C. of am. to edges of covering glass & at intervals of some hours observed some glands— they first became very finely granular which slowly became coarser, with small masses of granular matter; so as to be slightly opake; but after 5h not brown: the pedicels also became slightly less transparent, & the contents sank shrank a little from walls of cells; at upper end some transparent & rather large globular masses. — Evidently is not acted on nearly so quickly or energetically as Drosera.

[in margin:] (see p. 8 Back

(9

Old Flies

June 5' 8° 30' Under the old fly almost (quite) dry — both short & long glands full of g brown cellular matter. By taking in sti slices I am now certain wonderful contrast between the glands of both lengths which have been in contact with the fly & the others which have not —; all in content have cells of glands with brownish granular matter: fly itself very tender & segments segments of abdomen most easily separated.

Jun 5th Cabbage seed wh. had been on leaf cut into. 2 & another seed left for equal time in water cut into two. The former of a paler green (i e cotyledon & embryo —) than that in water; but greater difference was is in coat of seed, which instead of rich charcoal brown, viewed as transparent object oval, was much paler & lighter coloured & much disintegrated flocculent matter adhered to insides, instead instead of being almost clear. There can be no doubt much affected by secretion. The cotyledon & embryo more disintegrated in leaf the Pinguicula specimen.— (secretion still fully acid Jun 5th 9° 30' & still very copious.)

I have now compared slip of leaf at the end, where secretion lay, glands all with brown contents where at other end transparent —

There is no question that glands have absorbed matter from the cabbage seed. —by e by natural endosmose & exosmose.─

[9v]

the unicellular pedicel of long contain contain nucleus & nucleolus

[sketch]

Appearance of protoplasm after weak C. of ammonia within pedicel; occas spheres only occasional appearance

(10

Jun 6' 8° 35', 3 atomest atoms of meat on leaf by 2 Pins (9. 25 [+] 15' [=] 40 already much secretion)

2°. 15' I can see no trace of bending of adjoining glands)

5° do— nor does it increase the secretion of surrounding glands (7th. 8° Am no inflection — atoms not much reduced.) (2° 30', 2 of the bits of near rendered transparent & colourless but not dissolved.) (7th 6° 45' 2 of the bits of meat quite dissolved & disappeared] 8th 8° 15' I now find only 1 atom of meat completely dissolved, the other 2 dry remnants)

Meat & Cartilage

(Leaf 2 Pins)

7th 10 8° 13' A m. 3d bit of cartilage from leg-bone of sheep, just basally of the 3 bits of meat

6° 45'. I cannot see much action on the cartilage, that secretion was excited)

(8th 8° 15', the atoms reduced & rather rounded — one much reduced — & mere trace left) (8th 4° 30' the bit greatly reduced & now & I believe before completely softened or liquefied.) 8' 6° 55' the merest traces visible)

(9th 8° 45' of one atom I can see with strong lens the nearest vestiges. (6° 45' bit all gone)

(10th 7° 30' A.m. one bit has completely disappeared, but then I moved particle to closely adjoining glands.) (10th 6° 7 30' P. m. all the cartilage gone & all the the secretion absorbed.) (ie in 3 & 1/2 days)

(11

June 9th 8° on old leaf (red-stick) on which atom of meat fibrin was placed on May 31' now show traces of resecretion — I saw same yesterday on glands over which I had placed atoms of starch— the glands protruded & were beginning to resecrete (see p 7D foot)

Starch put on Jun 2d Resecretion.

June 10' 4° 45' Pm 3 seed of Luzula or Carex soaked for 3.4 of Hour.

Seeds Secretion

11' 7° 30' aft 15° great secretion from all 3 seeds, so that it has run into channel in one case

June 12' 8° A.m secretion almost ceased — 19 40° p do 24 [+] 12 [+] 3 [=] 39

(N. B Channel very useful in case of a shower wd save insect or seed being washed off leaf

(12

June 12' 8° A.m. Pea pollen on 3 places on leaf with red stick (13th 7° 30' secretion)

Pollen

Jun 14' 9° pollen-grain in furrow — not break — no tube emitted— mottled with fungus — discoloured & pollen green — some grain with fibrin greatly reduced as if absorbed — no doubt matter at least colouring matter absorbed — pale pale dirty brown

Examined the other spec pollen in same discolored state & in all respect the same— I removed at same time numerus glands & these exhibited splendid aggregation — a dozen or two of the shorter glands good & with a few exceptions of some empty ones the cells of glands full abounded with globular or almost spherical masses of protoplasm— have lost their pale green colour — Certainly wd feed on wind-blown pollen —

The fresh pollen in water is yellowish & not shrunk from walls in water & full of coarse granules

(over)

(13

Pollen continued

In the natural pollen there are some colourless grains. These are only granular in Pinguicula spec. & the contents not generally fill, after immerse in water — all this I can say safely as far paler & discoloured & absorption by glands

(I see some shrinking in natural pollen)

Jun 14th 17 11° Fat Stick with Blue paper Head

3 bits — (15th 8° Am no secretion)

15th 8° no secretion

18th 4° 40' put fat in water

(14

Pinguicula vulgaris

July 7. 74

9°. 10' AM Gluten & Albumen in furrow

Put two small [sketch]s of boiled white of egg {abt 1/20th in shorter diagonal} one near the midrib the other close to the margin — Leaf marked with a bit of paint brush handle

(Put two bits gluten hardened in glycerine & washed on the opposite leaf in similar situations—)

(3° great secretion from all 4 bits of substances— albumen cloudy rounded

(8th 8° marvellous secretion from gluten) I think both most reduced in margin, where secretion accumulates & form a little pool, at least win case of gluten — The other leaf rather old)

(9th 8° Am The albumen & gluten lying in the furrow far more digested than those on disc which are almost drying up. no doubt from the accumulation of gastric juice. There is certainly use of involuted edge viz to make a temporary stomach & is related to each waiting insect there — so highly useful in two ways) the bit of albumen in furrow quite rounded & much reduced— the others angular — 10th 8° 30' Gluten in furrow greatly reduced)

(July 11' 8° the bit of albumen in furrow beginning to dry up — rather more digested than others — Gluten much dried— rather more digested in furrow)

(12th 8° albumen in furrow not yet dry) (13' 9° not quite dry)

(over)

[14v]

I see of chain of little bits of meat which were placed in furrow — 2 of them have been moved out some way on to lamina)??

(15

(Pinguicula)

June 20' 7° 45' Minute shreds of pure fibrin on leaf by square sticks & on leaf with Plantago seeds.

(11° 30' has caused secretion) after 3° 45'

(2° ie after 6. 15', 1 quite liquefied, ie smallest bit, not so largest)

(21' 8° Both completely liquefied but I can see a little flocculent opake matter still on midst of secretion —

(22' 8° aft 48° I can see vestige of fibrin at both places

― 4° 30' I can now see no vestiges of fibrin on the 2 leaves)

48 [+] 8. 30 [=] 56° 30'

20' 4° 30' I put bit of fibrin under in channel Rumex seed

X 21' 8° I cannot now see even a trace of opake matter

1 1/3.

fibrin cause less secretion than from meat or albumen drying up after 30h

(1 (16

June 22'

Pinguicula

4° 40; P.m. 2 bits of same leaf each in 1 dr. of sol. of carb. of ammonia of 1 gr. to 1 oz & of 2 gr to 1 oz. C. of Ammonia

June 23 (i.e. 17h

9. 50 AM 1 Grain Solution Glands brownish green with distinct aggregation. The protoplasm has a very slow movement

10. 2 Grain solution — Aggregation more distinct & movement more rapid— Long glands observed in both these — short glands also aggregated—

Aggregn is of a granular character mostly

(17

Pinguicula Gum

26' 8° 18— 2 atoms of powdered gum above & below saxifrage leaf

9° 35' I certainly think gum cause secretion

2° 15' do do

(18

Jun 15' 5° P.m Grasses fresh from meadow

Bromus ─ Poa ─ Festuca (?) & 4th kind

& Bit of mountain grass

16' 8° h & 2 of the grasses no secretion in also grass some secretion (the ripest seeds almost most secretion) Poa & Festuca?)

Bromus hardly any or none

(16' 4° 40' bit of grass drying up & coiled round)

(17' 8° A.m great difference in amt of secretion Poa [molles] (?) best in Festuca 2d Dogs tail poor. Bromus very poor)

17' 7°, one of the supposed grass grass seed which excited very little secretion in a flower-bud!!!) (18 I now find a second case of young flower of Bromus pollen which I mistook for seed & with pollen shed & which caused more secretion than any) (19th 7° 30 Bromus blown off by a puff)

Plantago 16' 8° secretion ─ 17th 8° very little (21 8° Blown off)

Rumex 16' 8° A much secretion ─ 17' 8° less secretion ─ 24' 8° still a little secretion in planted seeds

19th Another supposed grass seed ─ turn out flower-bud only 2 tried Bromus & Poa or Festuca

/over

(19

Jun 16' 8° 10 Near tip of leaf large [illeg] of cabbage — smaller of spinach —

9° 50' 10 40' spinach some secretion. 4° 40' certainly secretion

(4° 40' some little secretion from cabbage)

17th 8° far more secretion from spinach than cabbage

none has run down from cabbage, whilst it has run down from spinach (& I have put atomest atom (starch) of starch in secretion)

(17' 7° Pm spinach drying up) (18th 8° bit of starch not dissolved) (19th 8° no trace of dissolution & I am now certain cannot in the least act on starch)

17° 24 [+] 11 [=] 35

18th 8° A.m find the only result are that of the seeds the almost ripe seed of the Festuca or Poa excited much secretion— the Bromus only a little— a third grass seed very little— Rumex considerable — Plantago very little (Plantago dry on 24' or 23)

21' 8° the ripe Festuca — & Rumex seed & even Plantago still excite secretion

24' 8° The secretion under Festuca & Rumex almost dry (planted seeds)

18' 8° Neither bit of cabbage or spinach discoloured but anyway changed that I cd perceive.

(20

Pinguicula

from Amy

June 23rd

Cut out 3 bits of Pinguic leaf on which a miniature sycamore & 2 glandular leaves had been caught—

(1) Sycamore piece; the little leaf lay near the border of the Pinguic leaf the strip was cut transversely across the midrib— CD observed that the glands were clear beyond the midrib but aggregated where the leaf had lain— In two other portions of this same piece of leaf (sycamore piece) I saw aggregation: in one rather more one the glands were clear at one end clouded at the other

(2) & (3) were cut close round the leaves: all the glands were much aggregated — more so than the Pin glands which were beneath the miniature sycamore leaf

The aggregation which I saw was chiefly in the short glands & very plain, just like under insect & under seeds. —

(21

(Pinguicula)

June 27th 8° Am seeds of Erica tetralix very minute (I think excellent ones) old seed soaked for 15°

5° 30' no secretion

28th 8° no secretion — either too small or too old & dry

July 2d 8° planted these 6 Erica tetralix seed & on opposite side about 20 fresh seed not soaked

July 2d. 9° 3 leaves of tetralix (to see if secretion)

(4° 30' have excited some secretion) — (3° 8° excited only slight amount of secretion but does excite some)

i.e. in 7° 30'