(2

The next day 18th was very gloomy & wet, the chloroformed Plant Oxalis kept it leaves open all day, whereas one unchloroformed plant kept them partly closed opening them in early morning, & now at night 11° a. P.m has not gone to sleep, whereas the other unchloroformed plant is fast asleep! Shows chloroform has produced some effect & was absorbed. — Shows chloroform has produced some effect & was absorbed.─

On other hand the Clover which was less chlorod. yesterday went well to sleep tonight, as if nothing had happened to it — it was less chloroformed than the oxalis

Oct 19th This night the oxalis has recovered & gone to sleep well at same time as other plant.— It is very odd the sleep being checked for one night.— 20th This oxalis opened in morning all right, I then put both plant in open air, bright, but cold & very windy; they closed their leaves partially →

[2v]

but when at 12° brought into House partially opened them. Was it cold in contrast From House or movement by wind?

Oct 20th at 4° 9' put old & large plant with leaves not well expanded under Chloroform & left it till 5° 9'; at 5. 25' & 6° 25' though covered by shawl hardly more asleep than when put under chloroform & less asleep than the other non-chloroformed plant; but at 7° well asleep; this was 2 hours after chloroform & proves that the vapour delays the plant going asleep. From experiment observation at (p. 1.) ought to have been asleep at 5° 15' or sooner.

It seems that sleep certainly delayed for short time by chloroform. — This plant went to sleep next night; so the first case some odd chance.

(3

Oct 18th Fresh Clover put under Bell-glass with Chlo. at 4° 39' at 5°. 39' a few leaves I think had gone to sleep; but it was previously intensely going to sleep. At 6°. 25' took off Bell-glass— not more asleep; nor at 8° 11° P. m: but poor plant & I am not very sure Certainly seem less asleep than other plant; but tall leaves show sleep best.—

Oct 19th the above dose of for 1°. 45' was too much, the plant seems seriously injured this morning —

Oct 19th I got 2 fresh plants of white clover at 4° 8' gave one 1/2 spoon of Chloro. & left till 4° 38' & took off glass; at 5° 15, I think some leave slightly asleep but not so plain as in other plant; but at 6° 10' all the several tall leaves (which alone go well to sleep) were fast

[3v]

Tri-filiform apparently does not go to sleep nor Sarforia —

(4

asleep, as well as in other plant. Now this was only at most, 1 1/2 hours after the chloroform, from analogy I shd doubt whether effect could have gone off. — Apparently slightly delays sleep. — Oct 20th awake all night next night this morning; & kept properly wake & at night went to sleep as usual, so did, as in first case of Oxalis, affects the sleep the 2d. night — (& 21' & opened next morning) Specimen now thrown away. —

23d Exposed fresh Clover for 1 1/2 hour to Ether viz for 4° to 5°. 30'; when I think it had gone slightly to sleep, as much as other plant

At 6° perhaps fresh plant rather more asleep, at 8° fresh plant more slightly asleep. At 9°. 30' the etherised plant well asleep.— Perhaps checks sleep.—

Oct 24th Put clover at 4°. 50' for 2° under Ether & certainly went as well to sleep as plant by side; kept on ether for 1/2 hour more to see if it would hurt plant. — /over

[From 3v:] Hence Ether does not stop sleep—

[4v]

Nov 1. at 5° P.m gave one plant 30 minims of Chloroform for 1° — at 6° & at 7° less asleep I think am almost certain than other plant: at 9° partially quite asleep, but still I feel sure less thn like the other plant. —

Nov. 2d. Next morning this plant after 1 hour of Chloroform was wide awake again & not injured.─