(2

The next day 18th was very gloomy & wet, the chloroformed Plant Oxalis kept it leaves open all day, whereas one unchloroformed plant kept them partly closed opening them in early morning, & now at night 11º a. P.m has not gone to sleep, whereas the other unchloroformed plant is fast asleep! Shows chloroform has produced some effect & was absorbed. — Shows chloroform has produced some effect & was absorbed.─

On other hand the Clover which was less chlorod. yesterday went well to sleep tonight, as if nothing had happened to it — it was less chloroformed than the oxalis

Oct 19th This night the oxalis has recovered & gone to sleep well at same time as other plant.— It is very odd the sleep being checked for one night.— 20th This oxalis opened in morning all right, I then put both plant in open air, bright, but cold & very windy; they closed their leaves partially →

[2v]

but when at 12º brought into House partially opened them. Was it cold in contrast From House or movement by wind?

Oct 20th at 4º 9' put old & large plant with leaves not well expanded under Chloroform & left it till 5º 9'; at 5. 25' & 6º 25' though covered by shawl hardly more asleep than when put under chloroform & less asleep than the other non-chloroformed plant; but at 7º well asleep; this was 2 hours after chloroform & proves that the vapour delays the plant going asleep. From experiment observation at (p. 1.) ought to have been asleep at 5º 15' or sooner.

It seems that sleep certainly delayed for short time by chloroform. — This plant went to sleep next night; so the first case some odd chance.

(3

Oct 18th Fresh Clover put under Bell-glass with Chlo. at 4º 39' at 5º. 39' a few leaves I think had gone to sleep; but it was previously intensely going to sleep. At 6º. 25' took off Bell-glass— not more asleep; nor at 8º 11º P. m: but poor plant & I am not very sure Certainly seem less asleep than other plant; but tall leaves show sleep best.—

Oct 19th the above dose of for 1º. 45' was too much, the plant seems seriously injured this morning —

Oct 19th I got 2 fresh plants of white clover at 4º 8' gave one 1/2 spoon of Chloro. & left till 4º 38' & took off glass; at 5º 15, I think some leave slightly asleep but not so plain as in other plant; but at 6º 10' all the several tall leaves (which alone go well to sleep) were fast

[3v]

Tri-filiform apparently does not go to sleep nor Sarforia —

(4

asleep, as well as in other plant. Now this was only at most, 1 1/2 hours after the chloroform, from analogy I shd doubt whether effect could have gone off. — Apparently slightly delays sleep. — Oct 20th awake all night next night this morning; & kept properly wake & at night went to sleep as usual, so did, as in first case of Oxalis, affects the sleep the 2d. night — (& 21' & opened next morning) Specimen now thrown away. —

23d Exposed fresh Clover for 1 1/2 hour to Ether viz for 4º to 5º. 30'; when I think it had gone slightly to sleep, as much as other plant

At 6º perhaps fresh plant rather more asleep, at 8º fresh plant more slightly asleep. At 9º. 30' the etherised plant well asleep.— Perhaps checks sleep.—

Oct 24th Put clover at 4º. 50' for 2º under Ether & certainly went as well to sleep as plant by side; kept on ether for 1/2 hour more to see if it would hurt plant. — /over

[From 3v:] Hence Ether does not stop sleep—

[4v]

Nov 1. at 5º P.m gave one plant 30 minims of Chloroform for 1º — at 6º & at 7º less asleep I think am almost certain than other plant: at 9º partially quite asleep, but still I feel sure less thn like the other plant. —

Nov. 2d. Next morning this plant after 1 hour of Chloroform was wide awake again & not injured.─