(51G.

Temp.

(6) Put in at 140 — no inflection or reflection; 3 or 4 glands in spots like porcelain — the other glands bright red; 11.3— put in cold water; 11.30. no inflect & put in C. of A.; 11.45 glands blackened & some seg. 2.30 the inner sub-marginals all well inflected, some seg. but spheres very small, & not extending far down the tents; under compound 4.30 the spheres are well defined, but the cells not transparent & appear filled w. brown pulpy matter; no current.

(51H

(7) Put in at 15° — to 15° 1/2; 10.58 put in cold water; tent. very red, glands like white porcelain but tinged w. red; outer marginals reflexed, but not strongly; inner marginals a little inflected; whole leaf flabby; 11.30 in C. of A.; 2. 30 all tents muddy brown, w. no seg.; 4.30 look with the compound & not a trace of seg; the sol. in which this leaf was put was much discoloured, w. did not occur in any other spec.

(8) For comparison put non-heated leaf in C. of A— 4 gr to 1 oz at 9°. 45'.; 10-40 glands intensely black much seg. beneath glands but no inflection, aggregation 4. 30 under compound spheres

[Insertion:] Show how distinct aggregation & movement are

splendid in transparent cells & I distinctly saw currents in the colouless fluid in which the spheres floated; I saw the currents only by the travelling of the smaller spheres— No inflection from strong C. of Ammonia

Aggregation

(a) (over)

[51Hv]

(a) July 22d 10° put 2 other leaves in the same strength of solution at 10° —July 22d 8° 10' excessively & discoloured whole leaf & gland but no regular inflection I remember preparing spec for George & putting 2 leaves in a strongish sol. I think only 2 gr to 1 oz & they never became inflected

(51 F.F.

(5,Bis)?)

Temp.

Segregation

Put in at 130°. 11.15 put in cold water; no inflect. glands rather bright red; (did not put in C. of A.); 11.33 under compound, there was trace of seg, which as often occurs in old leaves; I watched one elongated fine purple mass of protoplasm; & distinctly saw it divide into two, but the movement was very extremely slow & did not occur in any other protoplasmic masses; certainly no currents; I cut off in morning a small slip of this leaf, & in a few hours (but I do not believe this was caused by cutting off slip) the lamina was inflected, which I never before saw in a water spec.

A heat of 130° not causing inflect, & yet certainly not killing leaf is like too strong C. of A. not causing inflect, yet causing seg. as in case given above.