p 10

Ipomoea purpurea 10 flowers crossed by distinct plant gave 10 pods with 5.0 seed.

10 fl. fert. by pollen of same fl. gave 9 pods. of which one bad with only one seed. The 8 pods had on average 5.37 seed.

10 pods spont. fert. under net were not specially picked out as good & yet had average of 6.0 seed.

The seeds were weighted & by proportion 100 of the crossed seed equalled (by subtraction of seed in the balance) 93 seed fert. by own pollen & equalled 79 of the seed spont. self-fert; so that it is clear in both cases that the self-fert. seed are the heaviest. I do not think the vars can be in any degree sterile together.

(But Lett. put on quantity of pollen & the stigma wd get its own. Now I remember that Gartner says perhaps in Kenniss that too much pollen is injurious. & Mr Wooler says so. mem animals. Spallanzani? or Dumas?

Planted Sep. 3. 3° P.m. 15 seed of each lot.

On Sept 7th 12°. Six of the seeds of crossed with own flower pollen have swelled & become a lighter brown & one of this same lot swelled before the others & now at 3° P.m one has germ. & now at 10° P.m a 2d & 3d have germinated.)

(Sept 8th 7°. 30' 4th & 5th have germ.)

(Sept 10th 2° P.m a sixth Sixth & seventh has germ) 15th 7° 30 eighth)

(17th 2° P.m. ninth) (18th 10th & 11th, 12th) (21st, 13th & 14th.) /over

[96v]

Convolvulus

Sept 7th 12°

Of the 15 crossed with pollen from distinct plant now (Sept. 7, 12°) two have swollen.)

(Sept 8th 7°. 30' one germ.) (Sept 9th 2°. P.m. a second) 10th. 7° Am a third (11th — 3°. P.m a fourth germ) 13th. 7° 30' fifth & seventh sixth) (14th. 7° 30' seventh) 17th 2° P.m.) (18th 7°. 30 & 2° P.m. 8th & 10th.) (20th. eleventh.) (23d twelfth.) (25th thirteenth)

(see p. 10A for conclusion)

Sept 7th 12°

Of the third lot of 15 self & spont, fert, not one has swelled. Sept 9th. 8° A.m. Three now slightly swollen & not so black, & one germinated; 9° P.m. a second germ.)

Sept 10' 7° Am. a third. germ.) (Sept. 11th. 2° P.m a fourth germ.) 12th. 7° Am. & 2° Pm. a fifth & sixth germ.

(13th 2° P.m. a seventh 10° P.m. a eighth.) 16th two 10° P.m) (17th 7° 30. Am. 11th & 12th) (18th 12th & 13th) 19th fourteenth

Sept. 8th

Planted 2 seeds equally developed of two first lots.) (Sept 9th. 3° planted 2 others, equally developed one marked with zinc is a spont. fert. plant seed. (Sept 10th 7°. 30 Sept 10th 3°. P.m. Planted the third pair of seeds, equally germinated.

(Sept. 12th. 3°. 30'. Of the 2 first pair planted, the crossed have cast off old seed-capsules & expanded their cotyledons decidedly before the 2 seedlings which had been crossed by own flower's pollen. 14th on the third pair the crossed seedling had only very slight advantage over uncrossed.)

(In flower whilst young the stigma projects beyond anthers, but stamens grow rapidly, & though they dehisce externally, yet as they pass stigma leave some pollen on it.) N.B. this the self & spont. fert. flowers will be fertilised later than the crossed flowers. —

(Ipomoea purpurea)