(1

1866 June 18th 4° P.m. sowed seeds of Lythrum (No' 9), a Hom long-sty. plant from long-styled, fert. by other Hom. various seedlings; some seeds germinated at 7° A.m. Jun 30th.

do. date: seeds sowed of Hom long-styled plant, from a short-sty by own pollen, & some few germinated at 7° A.m, Jun 30th

do date, other seeds of from same parentage plant, but fert. by legitimate pollen from long stamens of leg. short-styled plant, one seed germinated at 7° P.m. 30th.

Lythrum Plant No' 11. Hom. mid-styled from mid-styled. —

Two lots of seeds; one lots fert by other Hom. plants & other fert by legitimate pollen of longer stamens of legitimate long-styled both germinated July 1' 4° P.m planted on 18th of June 3°. 30'— one seed in each lot. —

So no difference in these seeds of Lythrum

1A)

Red-equal-styled Cowslip— seeds self-fert. & seeds from both forms of common cowslip, Plant No' 27 both sowed on evening of June 19th. germinated both at same time on July 2d. in morning; if any advantage self-fertilised had it. —

(20th. 7° 30' Ten of crossed & three of uncrossed have now germinated)

N. B I find on reference that this plant was unusually little fertile with own pollen — so not good case— Both are in fact Het. Unions, but odd that pollen from distinct plant gave no advantage to seed—

(2

July 4' 7° P.m. Seeds of peloric Antirrhinum "Wonder" by pollen of same plant & other seeds from do by cross with common Snap-dragon

July 10' 7°. a.m. many of crossed seeds germinated.

― 12th 3° P.m. a few seeds of the self-fertilised seeds ✔ germinated — after i. e interval of 56 hours, & it shd be noted there were far more numerous seeds of latter, or self-fertilised.

Myosotis alpestris (see Portfolio about germination)

seeds planted in same glass on July 4th 7. P.m.

N. B. many more Twice as many of the seeds from under net pl laid on sand, than of others; so that chance wd favour the former.

July 13th. 7°.15' Am 2 or 3 of the crossed seeds have just germinated. —

13th 9° 30' P. m not one of uncrossed seeds yet germinated)

(14th 7° 25' A.m. — 5 seeds crossed now germinated, not one of uncrossed (3° P.m. none)) (16 17th 7° 30' A.m. six of crossed seed have germinated & not one of others, though so numerous.)

(No doubt seed under net, though looking as good, may be less healthy as plants were, but this source of doubt will be eliminated in other cases. In some exper. same single tried, in others several, so average got from mixed seed, from several capsules, wh. likewise eliminates ripeness.)

18th. 7° 30 A.m. Seven of crossed seeds now germinated. /over

[2v]

Myosotis

(July 18th 8° P.m. one of uncrossed seeds has just begun to germinate — so interval 5 & & ✔ 13 h.)

(July 19' 7° 30' A.m. another uncrossed has germinated.)

(20th 7° 30' Ten of crossed & three of uncrossed have now germinated i. e. in one week after first generation.)

(Jul 21st. 3° P.m. 13 crossed & 5 uncrossed germinated.)

Aug 24' The young plants left in glass & uncovered pot (for I did not think about growth) & perhaps not equally well treated) but the crossed plants are now more than twice as tall as those raised under net—

(2A

Myosotis alpestris 1867. Some plants were covered by net, & some left free to bees; the latter produced very many more seed. — perhaps merely owing to free application of pollen.—

(May 10th 1868 the self-plants, now about 2 inches to tips of leaves, are rather finest)

(May 9th 1869. Since above date the plants are quite equal & now in full flower, no difference between self & crossed in any pots —

(3

July 26th. 3°. 30' P. m. seed of Cowslip-Poly, long-styled, flowers fertilised by pollen from same flower, not very sterile — The parent plant was child of a long-styled Hom. plant, & this was also child of first Hom. union; so that 2 Hom. generations & their seeds & a will produce plants which will be great-grandchildren, all Hom. generations (& last. gen. by own-flower pollen) from 1st. Hom. Union.

July 31' 7° 30' A.m. after interval of only 4 days + 16 hr 2 seeds had cracked envelopes & in 3 or 4 hours 2 more had done so

These plants germinated more freely or in greater numbers than those below. —

[in margin:] The parents of the seed grown in greenhouse.

For comparison sown in same glass, seeds of Cowslip-Poly, which had been spontaneously fert by both forms growing close together (see Exp. Book p. 79); but those plants were children of long- & short styled Hom. plants heteromorphically fertilised.

More than twice as many of these seeds sown. —

✔ Aug 2d. 10° P.m. interval 2 days 14 hr. one seed germinated (16 seeds of other lot have now germinated & yet not nearly half so many planted). (over)

[3v]

These facts on Homomorphism (I think something of kind supposed with P. Sinensis see) at least show that crossing had some powerful influence on germination. —

(Two similar lots of these Cowslip-Poly seed (of p. 3) sown in same manner in two pots in ordinary manner, & treated exactly alike in Hot bed — & on Aug' 11th in evening one plant of the spontaneously crossed plants come up & Aug 12th 2 or 3 one of the self-fert. came up. — Now Aug 13th 4 or 5 of crossed plants are up & only 1 of self-fert.; shows sowing in soil not to be trusted for germination.

Aug. 18th Now about 60 of crossed plants are up & have leaves & only one of the Homomorphic self-fertilised seed. — Yet in experiment of p. 3. all seeds of same actual lot: after germinating a multitude must have died in the pots. — very curious case. — Aug 20' The one plant has died & another come up. I find several have germinated & then died— I think only too dry

(4

on July 15th 4°. 30' P.m. seeds were sown on sand in glass of common cowslip which had been homomorphically fert. by a long-styled Homomorphic Cowslip-Poly. & on Aug' 1st 7°. 30' 3 or 4 seeds had cracked. (Interval of 16 days & 15h. — Now these were seeds of 1865 & in last experiment where Hom. seeds germinated in 4 days 16 h. they were freshly gathered.)

For comparison seed of long-styled cowslip Heteromorph fert by short-styled of same plants sown but few of either lot. — Sept. 17th 21st not one of this lot has germ — so whole case valueless.

[4v]

About germination of seed, showing that crossed generally germinated first but no rule

(4A

Long-styled Cowslip-Polyanthus These are P. Sinensis experiment

June 1868 — Four of the 20 long-styled gr-gr-grandchildren (mentioned at p. 423 of my paper see Exp. Book p. 79 for planting — Illegit. Progeny) Cowslip-polyanthus were kept in greenhouse, & 30 fl. were self-fertilised & yielded 17 pods, containing on average 32.0 seed with max. of 50 & min. of 20 — seed mostly fine & good — This is surprising fertility for illegitimate union, now many times repeated — I think, (compared with my paper p. 426,) clearly this shows fertility is increasing, as with red-equal-styled cowslip. — (p 4B work & reference)

(15 fl. were legitimately crossed by short-styled cowslip-Pol but yielded growing in greenhouse see (I think owing to accident of several umbels perishing) only 5 pods, containing average of 33.25 seed with max. of 56 & min. of 21. — Hence only a very little more fertile than the illegitimate union, but too few pods to judge. — Growth will be very interesting. —

Four pots sowed on opposite sides. —& plants thinned out to be equal on opposite sides. —

(4B

Cowslip-Polyanthus. C. C. C. C.

The plants mentioned at foot of last page in the several Pots, now 2 1/2 inches to tips of leaves, show no marked difference on either side, but in Pot III. self have the advantage.)

(March 7' 1869. The plants in all 4 Pots are just beginning to flower (Seven or 8 plants are long-styled but two are short-styled!!!)

Pot I crossed flowered first

II. Self. flowered first

III. do. do.

IV Crossed flowered first} so half & half

I believe I have mistaken, owing to labels, the crossed & self- side; anyhow more in all 4 Pots the crossed look in an unmistakable manner far more vigorous, the leaves being at least 1/3 broader gr gr gr grandchildren & much darker green. —)

(March 14th 1869. I have now measured the breadth of finest leaves in all 4 Pots.

April 23d. 1869

[table not transcribed]

April 23 The crossed plants look altogether larger, more vigorous & greener. —