(49

Escholtzia Californica

Crossed 12 fl. Self-fert. 18 flowers.

The 12 crossed gave 12 pods, but one contained only bad seeds— the seeds of the 11 pods weighed 17.40 gr.

The 18 self-fert. gave 12 pods & seed weighed 13.61 gr.

Hence of crossed pods flowers 91 per cent set, good pods

― of self-pods flower only 66 per cent set good pods.

By proportion, the weight of seed produced by crossed pods is as 17.40 to 12.48 or as 100 to 71.1

Hence of 2 plants with equal number of flower, if one had all crossed & the other all self-fertilised, the yield of seed would be as 100 : 52.

The plant produced a good many pods spontaneously:

Fritz Müller found single plant in S. Brazil produced no seed. (Crossed seed perhaps germinated rather the first)

(May 10th 68 — Plants in all Pots — about 5 inches to tips of leaves no difference)

(May 24th — 8-9 inches high & still equal.)

(June 6. 68 Pot 2. self- flowered first.)

14 ― Pot I Crossed first—

(Some germ seeds were planted on each side of Pot of Cineraria Crossed 22 1/2 — 16 1/2

Self 13 — 9)

(1) The Plants in pot sterile in [illeg]

Cut down a [2 words illeg]

50

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(50

Escholtzia. July 5. 68 the growth of the tallest flower stems measured

[Table excised] Plants in Pots in greenhouse

1868. Some Plants in the Pots were experimented on, & so sterile

6 fl were crossed & gave 3 pods, with moderate lot of seeds—

12 flower were self-fertilised by Lettington only 2 flow pods were spontaneously & self-fertilised produced & these contained very few seed — the best of 2 with only 14 seed.

No pods were produced spontaneously.—

The above plants on same pods were kept, after dying down, & have now (May 2 1869) flowered a second time : in Pot. I, II, & III, the flowering stem of the selfs are tallest & they flowered first. In Pot IV the crossed are finest.

(When a plant flower has really fertilised itself very early, an application of pollen from another plant disturb & injure old pollen: I doubt.) from common action of insects in all cases.

(50A

Eschotzia. — 1869.

(May 16. The young Plants of present second generation, now 2-3 inches high to tips of leaves seem equal on both sides.)

(June 6. 1869, young Plants now about 8 inches to tips of leaves & about equal in all Pots on both sides.)

Aug. 2d. Measured to ends of Pods on flowers. plants fully grown.—

[table not transcribed]

In Pot I, II, III the crossed flowered first, in Pot IV both sides flowered at same time)

Aug 2d. (I counted the expanded flowers & pods

The crossed had flowers 10 pods 27 [total] 37

The self-plants had flower 11 pods 23 [total] 34

So no difference in pods produced.

50A

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(50D

Eschotzia Californica seedlings from Brazilian plants raised in this country, by crossing together the parent Brazilian plants & self fertilising them: I wish to see whether this strain, which is much more sterile than our English strain, differed more in growth than our English plants when raised from crossed & self-fertilised seed.—

(August 8th 1870)

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When added up it will be seen that there is hardly any advantage on side of crossed.

Three of the Pots were covered up & they produced no pods spontaneously; but as plants were not healthy, nothing can be judged those in Greenhouse produced very few.─

50D

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[50Dv]

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(50E

Eschotzia. Cal.: Plants on last page (which are grandchildren of Brazilian seed) were then spring 1870 self-plants again self-fert. so 2 generation of self-fertilisation & marked with black thread; & crossed plants again crossed inter se, white thread; & likewise crossed with pollen of English Eschotzia white wool. to see whether this greater change wd do any good.—

8 flowers with Black thread self-fert — produced 5 pods

8 ― White Thread [illeg] — produced 5 pods

8 ― white wool English — produced 4 pods

Though so few pods can hardly doubt the cross by English plant has increased fertility over that of the intercrossed.) These 3 kinds of seeds will soon (Ap. 21 71) be germinated on sand.

(April 28th the self-fert seed certainly geminated at a quicker rate & more freely within equal time than either of the crossed seed.)

(May 22d 1871. Plants 2-4 inches high to tips of leaves & they present no marked difference of any kind; except that those of crossed by English plant in row in garden, grew came up rather more vigorously

(50F

Escholtzia californica Plants raised from seed described in last page, p. 50E. ─ July 20 1871

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Plants in full flower growth almost, but not absolutely complete, measured to extreme tip of upmost leaf.─ Red cross mean flowers first ─ So not one of intercrossed flowered first ─ (yet see about germination)

(N.B. There is one less set intercrossed Plants)

Average of crossed by garden 34 7/8

─ Intercrossed 32 6/8

─ of self fert. 36 4/8

As in Mimulus intercrossed shorter than self!!

See next Page

x] 50F

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(50G

Escholtzia Cal. Aug 28 /71/. Same plants as last page, now fully-grown, measured to tips of leaves, in Pots in green House — drawn up

I cut off all these plants close to gr soil & weighed them, the pods havng been removed.

The 19 plants of Crossed by Garden plant weighed 18 1/4 oz

The 18 Intercrossed plants weighed 17 1/4 oz; (so 19 wd. weigh nearly the same. (viz 18 +)

The 19 self-fert plants weighed 21 1/2 oz —

So self-fert. plants much taller & heavier than the intercrossed & those crossed by Garden plant. It will be curious to see about fertility— If no advantage here no advantage of any kind— But I suspect, as in first cross so there will be here advantage in fertility, but not in growth.

Weigh without pods one less intercrossed Plant

See Hildebrands paper on sterility of Brazilian Escholtzia. in Bot. Zeitung (over)

[50Gv]

The 19 plants in the 7 Pots crossed by garden plants produced (including 12 in paper of which seeds have to be counted) up to Aug 28, when all small & great were gathered 240 Pods— The greenhouse not favourable to fertility.—

The 18 intercrossed Plants produced 130 pods (so that the 19 wd have produced about 137 137.22 pods calculate this accurately)

The 19 self plants produced 152 pods — so again the intercrossed show disadvantage.

(The refuse seed, more or less germinated, was sown in 3 adjoining rows in open ground in same soil; now Oct 3. 1871 the self plants are 2 or 3 inches taller than either of other 2 lots. — Of These are equal, or I think those crossed by garden plant perhaps a little finer.)

(June 1872, the plants raised from this refuse seed were left unprotected all winter, & oddly enough, two of the self Plants alone survived & are now in flower─

50G

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