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Viola tricolor. Three plants, very similar, seedlings, large-flowered, purple & yellow. The pollen is wholly shed from anthers of young flowers & collects in a little pocket or semi-cylinder, formed by narrow incurved base of lower lip, where it is surrounded by dense mass of papillæ. It lies ready to be removed by proboscis of Bees, & to be forced into cup-like stigma.

10 flowers crossed, produced 4 capsules with, 17, 15, 33, & 17 seed.

10 fert. with pollen from same fl. produced only 2 pods with 19 & 3 seeds.

Nov. 23d. 67 Pot I, II & III. 6 or 7 seeds of self-fertilised sown on one side, & more crossed on other side.

Dec. 8' in Pot I self & some crossed have grown, at same time.

(April 3d 68 In one Pot viz II. alone one self- & two crossed came up: of the 2 crossed one equals the self, & the other about 1.3 taller than self.)

(April 23'. 68) Both crossed plants flowered first before the self. — appear equal in growth)

(July 5 68 The tallest crossed flower stem 12 1/2 inches high

do self [do] 8 [do] [do])

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During summer of 1868 Lett. crossed several flowers on new plants & 6 pods gave average of 17.5 seed with max. of 29 & min. of 7.

Several flowers art. self-fert gave 10 pods with average of 13.2. seed, with max. of 29 & min. of 5.

Hardly enough to be trusted. — Germinated on sand & grown on opposite sides of Pots.—

(Sept 20 /68/ The crossed seeds have germinated first & best; many of selfs became mouldy.)

(Dec 6 /68 Plants in several pots, now about 2 1/2 inches high to tips of leaves, & the crossed in all 4 Pots have decided advantage & look larger & finer plants.)

(March 14th 69. The advantage is decidedly kept.)

Pot. I crossed fl. first. Pot II. self-flowered first. Pot III — crossed flowered first. Pot. IV. crossed first. Pot V. crossed flowered first.

& 2 plants in a [2 words illeg]

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Viola tricolor 1869

April 27 — 1869 — measured to tip corner of little leaves at end of latters stem

June 2 1869 ─ most Plants nearly done flowering

[table not transcribed]

April 27 The plants have flowered yesterday in Pot I, II & III.

*The self plants in Pot III (Jan 2d 69) are yellower & less [illeg] than the crossed

June 2d 69 Owing to plant kept in greenhouse, with few Bees & confined in growth, only few pods produced; & these few confined to Pot III & IV & on crossed plants alone.─ /over

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V. tricolor. Sept. 27. 1869

The plants in Pots were bedded out of doors & the difference in the number of pods produced perhaps best shows wonderful difference in size & vigour; but some of the few pods produced contained by the self plants contained fully as many seeds as the pods on the crossed plants.─

Pots.	Crossed	Self
I	22	0
II	22	4
III.	76	2
IV	39	2
V	8	9
Total	167	17

Of the above Pot I & II were left in Pots, & 3 other Pots were turned out with balls of earth unbroken

April 24 1870 The Plants all in these 5 Pots, after being left all winter embedded in open ground — now present so great a difference, tha in size & vigour, that they cannot be compared. The crossed are all a mass of bloom & only the self in Pot II. (which are now very much smaller than the crossed see last page) have flowered at all. In Pot the self almost dead & dwindled to one minute stem.

(Ap 24. 1870 As Pt. II. on self- side was in last page, unusually fine, I have now carefully examined it: it is now only in bulk or diameter of whole tuft or crowd crown only from 1/8 to 1/10 of size of crossed Plants : the tallest flower-stem are 6 1/2 inches, whilst the crossed are 8 inches high.)

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Viola tricolor. Aug 1870.

[Table partly excised, not transcribed]

The seeds from which these plants have been raised were spontaneously produced in open air, from the tall crossed plants & from short self-plants, of last first experiment & no doubt were crossed. The difference no doubt must is due to inheritance.—

The 2 tallest plants on each side of Pots were alone here measured— up to tip of calyx of flowers; all having flowers—

In all 3 Pots the crossed flowered first. —

March 24 1871. The last winter 1870-1871 severe & the selfs all killed in all the pots, (the crossed not the least injured) except in Pot IV in which one self plant has a rosette of leaves about 1/2 size of pea still alive.)

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[calculations not transcribed]