(66

Nolana prostrata (old notes in Dichogam or Dimorph. [illeg] Germination of seed Portf Portfolio)

I believe 30 flowers crossed, 27 pods produced each with 5 seed (In greenhouse) (1867)

32 flowers were crossed with own-pollen (26 having been crossed from same flowers & 6 from flower on same plant, but the latter plant not the least advantage) These 32 flowers produced only 6 pods each with 5 seed.— so very sterile with own pollen. In 3 cases 2 seeds had become confluent & formed one gigantic seed, counted as 2 seeds. —

26 crossed seeds weigh 4.05 26 self seeds [do] 3.33} Hence crossed seeds in weight, one in equal numbers are as 100 : 82. —

(May 24' /68/ Plants 6-7 inches high equal.)

(June 7' 68 Pot. 3 — crossed flowered first.)

June 12— Pot. 2. self- flower first

14— Pot I self — first.)

(July 8 /68/ Plants now setting seed.

	Crossed	Self
Pot I.	8 1/2 4/8 6 1/2 4/8	4 1/4 7 1/2
II.	10 1/2 /48 18	14 1/2 4/8 18
III	20 1/4 2/8	22 3/4

 

the 2d Plant in this pot was bitten off by insect when half grown.

63 6/8 46

[78]

[calculations not transcribed] Very unequal

[78v]

[Fragment of draft of Descent?]

Yet very early migration

depended on the in

in short obvious th

over other animals.

one tribe over another

[79]

July 6— 62/ Nolana prostrata vide Ch. Fermond?

Long-styled stigma apparently ?? larger & more covered with little dark papillæ—

Pollen about 14/7000 or 15/7000 in diameter of both kinds

(Some flowers have pistil decidedly clear above stamens — others have stigma exactly equal in length to whole group — & many have one stamen longer than others & the stigma on a level with this one & above the others — And there are some which graduate; case like Oxalis acetosella.—

Long-styled wire — Short-styled white stick

3 4 white long-styled by pollen of equal styled

4 black by pollen of long-styled from another plant

1 white}

1 black} July 9th July 10' 2 white 2 black (altogether 7 of each cross)

[79v]

July 13 saw Hive-bees collecting pollen on Nolana — saw many eagerly at work.—

2. wires means strongly long-styled

2 sticks. ― short-styled

stick & wire doubtful.

[80]

I see that there are often 2 longer stamens = to stigma & 3 shorter. I think commonest type on same branch apparently is one longer stem equalling stigma— but latter is level with 2 long ones; on the stigma is on level with the 2 or 3 lower ones; for all 5 stamens are sometimes unequal. — Generally same type prevails on same plant; but not always.

Had I known this latter fact, I would not have tried any crosses. —

July 28. I have reexamined plants— Certainly some have all long pistils, some all equal to stamen l but on some few plant some variability & scarcely possible to class the plants. —

[81]

Nov. 27 /62 Nolana prostrata

9 Plants uncovered, seed weighed 79 gr. ∴ 12 plants would have produced 105 gr. ✔

12 Plants under net in Greenhouse

white thread 3 seeds

Black thread 4

all the other pods had opened & got mixed with remaining seeds; so that 14 flowers were artificially fertilised in this lot. — Every single plant of the 12 produced some seed, so that none can be strictly dimorphic. —

These 12 Plants produced only 64 gr, & yet 14 flowers were artificially fertilised; so that Bees aid fertilisation considerably by laying on pollen on stigma.—