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Nitrate of A

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I then tried a good many experiments by the immersion immersing thirty-seven  x 94 = 61 + 2 = 63    cut-off leaves in same quantity of solutions of different strength.   & the result seem to me so remarkable that I shall enter into considerable detail [3 words illeg] all the experiments.

Having found that the minims of a solution of one grain to 12 ounces caused strong inflection, I then tried to experimented on six leaves in a solution of 1 gr to 18 oz of water, twenty-three  leaves in a solution of a gr to 24 oz.; & six leaves in a col. of 1 gr to 30 oz.) In speaking of the successive periods when inflection occurred, I always reckon from the time of first immersion.) (B)

(I first placed a leaf in 10 minims of a third solution 1 qr to 18 ounces, & after an interval of 48' a considerable number of tentacles were inflected: after an interval of 3° 13' (always reckoning all the tentacles were strongly inflected, & except some of the marginal, strongly inflected, with the glands

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(B)   In all cases the distilled water was the same for making the solutions & for immersing the leaves in simple water as a standard of comparison.

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all blackened, next morning after an interval of 21° the tentacles were in the same state condition. At the same time I placed a leaf in the same quantity 10 minims of the distilled water & in this as in all other cases treated the treated the leaves leaf in the same manner, as that placed in the solution.

After an interval of 3° 13'; twenty tentacles were found inflected but only to a partial extent & almost all of them were marginal tentacles with elongated glands, such tentacles as previously stated, are quite commonly affected by pure water, & have always been disregarded by me in judging of the action of any solution. After an interval of 21° several [illeg] of these tentacles had reexpanded.

(Give first)

(I then placed five leaves in 30 minims of the same solution viz. 1 grain to 18 ounces. After an interval of 2° 10'

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It is, I think, impossible to compared after the same successive intervals of time, the state of these six  5  leaves in these two experiments, at successive & not admit that those six [2 words illeg] exposed to the solution had been strongly acted on by the

As the five leaves were immersed in 30 minims of the solution,

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These assuming that they absorbed equal quantities, as is probably from the  [2 words illeg] blackening of all their glands; each leaf could have obtained only the 1/1460 of a grain of the [illeg]

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& each of which received the 1/1152 of a grains of [text excised]

I repeated this experiment & placed four three other leaves altogether in 30 minims of the same solution; but did not & watched them through a lens; in 5' I saw the tentacles beginning to move, & after 1° 15'. all the tentacles except nine, one the all these leaves except 9, were inflected; & after 8° 15 all except 5 were closely inflected. I counted the tentacles on these three leaves, & found the numbers to be 139 139, 173 & 175, which (subtracting to five) gives an average of 160 for each leaf. Hence

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Each gland could have received only the 1/185320 of a grain. I tried other analogues experiment not the same results, but with [2 words illeg] one [particular] cases. I also placed a single very fine leaf in ten minims of the solution: in 40' all the tentacles, except the marginals, were well inflected. After 6° all excepting seven marginal were inflected; & after 8° all excepting four four, which seemed injured, were inflected. I counted the tentacles & found the number 199, & subtracting the four, the each of the others could have received only the 1/224, 640 of a grain of the nitrate.)

(Then experiments on nine leaves, each receiving ten minims of the solution, & [illeg]   of which received the   1/1152 of a grain, as the most striking that I obtained with the solution of one grain to 24 ounces of water; but they are less striking than made with the solution of one grain to 18 ounces, for the leaves each leaf in the latter case received only the 1/1460 of a grain. The difference may, I believe, be accounted for by the great dilution strength or less dilution  to latter solution then the other, independently of the absolute quantity of the salt

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received by the leaves I conclude so from two experiments, in which I gave a leaf leaves the 1/1152 of grain, but ad doubled the water, & thought a good many tentacles were soon inflected, yet the result effect was not so conspicuous as in the previous foregoing cases.

As partial or negative results with from lesser [illeg] of the [illeg] or from weaker solutions are almost as significant from judging of a forming a judgement, as positive positive results, I made two trails with leaves immersed in seven minims of the solution of one grain to 24 ounces; & though a plain effect was produced with t in [illeg] cases were all the tentacles seen not inflected. I then placed five leaves in pure water for comparison & five in 30 minims of the same solution of one grain to 24 ounces of water. Hence each leaf had 6 minims & on an average of they had absorbed equally [2 words illeg] received whole probably was not the cells, so would each have received only the 1/1920 of a grain; & assuming that this average number of glands on each leaf was as in four cases 160, for on each leaf, each gland could have received, if all absorbed equally would have received   only the 1/307,200 of a grain. Now of these five leaves after immersion for 1° one had almost all its tentacles inflected, then others had many & the [illeg] only two or three affected. After an interval of 6° 15'

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the one remained in the same state, but the four others had begun to reexpand & had only 22, 23, 27 & 7 tentacles inflected,; & after 24° four others had all their tentacles fully expanded. These leaves, however were very much less affected that in the expanded former previously given   For comparison I immersed at the same time five leaves in distilled water : after 1° almost all the leaves had some few marginal tentacles inflected, & one had a very few round headed over submarginal tentacles at one spot inflected; but the contrast were great well-marked with those [illeg] immersed for the same time in the solution were well marked. After immersion for 6° 15' th one leaf had not a single tentacle inflected : after 24° all the tentacles were fully reexpanded.

(Finally I immersed five leaves altogether in 30 minims of a weaker solution than any hitherto employed, viz one grain of the nitrat to 30 ounces of water. If each leaf & le the leaves & tentacles had absorbed equally, each leaf would have received only the 1/2400 of a grain; & each gland only the 1/384,000 & after immersion for 4° one leaf, which I presume was to [illeg] a very active one & absorbed a larger an unfair quantity

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of the [illeg] had almost all its tentacles inflected; the second had all the extreme marginal & many sub-marginal inflected; the third had these marginal & several sub-marginals inflected; the fourth had only seven tentacles inflected; & the fifth had none, but all the glands on the disc of this latter leaf were blackened, [illeg] proving that there had been some absorption. After immersion from only 6° 30' many of the tentacles began to reexpand.

Five leaves were at the same time immersed in pure water: after 4°, two leaves had none of their tentacles inflected; a third had two marginal & three sub-marginals inflected; a fourth had two marginals inflected; & the fifth, had two marginal & several sub-marginal inflected. After immersion for 6° 30 all five leaves had altogether [illeg] only three tentacles inflected. Hence there can be no doubt that thus so [illeg] [illeg] a dose as 1/2400 of a quantity of the solution of one grain to 30 ounces of water, which if absorbed equally would have supplied only the 1/2400 of a grain to each leaf, produced some effect on all fives leaves but was not sufficiently strong  not enough to cause the inflection of all the tentacles on any one leaf.)

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Aggregation

(Used)

(I did not make my observations on the power of the nitrate to cause the segregation of in the contents of the cells beneath the glands, but enough to show that this salt, though more potent that the carbonate in causing the inflection of the tentacles, is inconsequently very much less potent in inducing segregation. I placed two leaves, each in ten minims of a solution of one grain of the nitrate to 12 ounces of water; & examined the one after 15°, & the other first after 6° & then after 24° & the no marked degree of segregation had occurred I then placed the latter leaf in a weak solution of the carbonate for 1° 45' & then now the cells in all the tentacles for half their lengths showed an astonishing degree of segregation; the purple fluid being conserted converted into spherical & elongated massed of protoplasm floating in a colourless liquid. I then